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CTAB (cetyltrimethylammonium bromide) / isooctane / n-pentanol performance anti-micelles extraction of bovine serum albumin (BSA), the paper mainly in three aspects. First determine the reverse micelles extraction BSA optimized experimental conditions; then reverse micelles, the anti-BSA extraction; final part examines the CTAB / isooctane / n-pentanol specific selectivity for BSA. Extraction experiments focused on the following two points. (1) examines the surfactant concentration, raw phase pH, ion concentration and type, the proportion of co-solvent, the extraction temperature, stirring rate, and oil and water than the extraction rate of bovine serum albumin, and found the better for BSA extracted experiment condition. The experiments show that the electrostatic interaction is the main driving force of the extraction process with the raw material phase ion concentration increased, the extraction rate is reduced, this is mainly because the ions are generated between the reverse micelle cores and BSA shielding effect. Appropriate proportion of n-amyl alcohol contribute to the improvement of the extraction rate of BSA. 0.02-0.04mol / L, pH 9.0-11.0, the concentration of the surfactant (CTAB) of the ion concentration of KCl / NaCl 0.05 - 0.2 mol / L, the proportion of n-amyl alcohol 0.2-0.4, at room temperature, stirring rate 200-300rpm, oil and water ratio of 0.67-1.5, the higher the extraction rate of BSA up to 98%; (2) for further experiments, a simple stripping experiments prove BSA remained after stripping better activity. BSA stripping experiment is based on the extraction experiments investigated the stripping conditions (stripping aqueous phase pH, ion concentration, ratio of oil stripping, stripping time) BSA stripping ratio. Obtained by experiments, the BSA concentration in the raw material liquid is lower, the higher the rate of the extraction and stripping rate. When stripping the aqueous phase pH is close to the isoelectric point of BSA, etc., the highest stripping rate, which is mainly due to the extraction process is controlled by the electrostatic force. Conditions to select the appropriate circumstances, the stripping rate up to 80%. And by experiment that the aqueous stripping phase ion at high concentrations (> 0.4mol / l), not only unable to improve the stripping rate, can also cause denaturation of BSA. End of the paper through separation experiments described in certain experimental conditions, CTAB / isooctane / n-amyl alcohol of BSA having a certain specific selectivity for α-amylase extraction experiments and BSA, a mixture of lysozyme, according The different isoelectric points for the extraction and separation of the target protein. Bovine serum is a complex mixture, in addition to containing a main component of bovine serum albumin globulin, hormones, amino acids, glucose and other ingredients, further comprising, homemade reverse micelle system mixed solution was extracted from this bovine cattle serum albumin, and reuse.
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