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Microbial Community Structure of the AMD and Their Dynamics under Different Conditions of Enrichment

Author: DaiZhiMin
Tutor: LiuXueDuan
School: Central South University
Course: Biological Engineering
Keywords: AMD Microbial community structure Restriction fragment length polymorphism analysis The phylogenetic tree analysis Bioleaching
CLC: Q93
Type: Master's thesis
Year: 2007
Downloads: 162
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Abstract


Acidic pit water (acid mine drainage, AMD), usually refers to the mine waste tailings leaching water, they showed a particularly acidic, containing high concentrations of metal (iron, aluminum, manganese, or other heavy metals) and non-metallic ions has become a huge economic and environmental burden. The autotrophic bacteria, such Aciditiobacillusferrooxidans, L.ferrooxidans A.thiooxidans and A.caldus such as acid mine water, there is a variety of prokaryotic mineralization. AMD in the presence of diverse flora together the combined effect of accelerating the oxidation of the ore, causing tremendous environmental pollution. Biological prevention strategies as an of today the AMD wastewater treatment effective method one study there is great significance of the AMD microbial species and its structure. Dongchuan copper mine is located in the eastern part of China's Yunnan province, has more than four hundred years of mining history, the altitude of 1800 meters above the perennial low temperatures. Dongchuan copper mine for a typical sedimentary copper mine, the rich resources of its implication, is China's most famous native deposited copper mine. Study of the Dongchuan copper AMD microbial community structure conducive to prevent acidic wastewater, environmental protection, and for industrial processing. This paper mainly studies the Dongchuan Copper Mine geochemical properties of the three plots, through the establishment of the 16s rRNA gene clone database to evaluate the community structure of acidic waste water, to discuss their geochemical properties, temperature, pH, and ionic concentration of the microbial community the impact of the structure. Restriction fragment length polymorphism and 16s RNA PCR amplification and sequence analysis of microbial community structure in Dongchuan, Yunnan Province, China chalcopyrite acidic leaching wastewater (AMD). Based on the establishment of the 16s RNA molecular clone library method often used to study microbial ecology AND 16s rRNA gene was amplified by PCR, cloning, and then to filter out the different kinds of restriction fragment length polymorphism. Three plots of selected 357 clones, restriction analysis clustered into 100 operational taxonomic units (OTU). An OTU represent the individual samples to appear digestion patterns of the same kind. 58 OTU sequencing analysis results than in the gene database analysis, the phylogenetic analysis showed that they clustered into six categories: γ-proteobacteria, Acidobacteria, Clostridia, Actinobacteria, Nitrospira, α-proteobacteria. Among them, γ-proteobacteria largest population in the three plots DCK-I, The DCN-I and DC-II, respectively, accounting for 36%, 57%, 62% proportion. Leaching microorganisms such as At.ferrooxidans; L.ferrooxidans but found very little, only found four of The OTUs in each of the two plots. In the the AMD environment immersed mine microorganisms are not dominant populations. In view of the AMD microbial community by the limitations of matter in the end, the research community under different nutritional conditions change, helps us to understand the formation of AMD from selective screening metabolically efficient strains of a particular substance, the use of different Acidic the bioleaching water, energy material culture mined by analysis of the changes in the microbial community structure before and after culture found in the microbial community structure of different substances as an energy source, cultures vary, for example in the use of ferrous for the the major microbial energy for Aciditiobacillusferrooxidans bacteria, other microorganisms such as L.ferrooxidan, Acidiphilium sp may also exist, but only a few exist. Use of elemental sulfur as energy substances Shique Acidithiobacillus ferrooxidans, and Leptospirillum ferrooxidans other but did not find Gaja iron plus sulfur powder for energy substances Shique Acidiphilium sp Acidithiobacillus ferrooxidans, ferrous sulfate, sulfur powder plus yeast extract culture Shique Leptospirillum ferrooxidans mainly microorganisms present, both the presence of small amounts of other substances. Using the original AMD wastewater using the 9K medium enrichment culture in the leaching process, we found that its leaching effect because of their different pH and pulp density changes from low to high changes in the pH of leaching effect change from high to low, while the pulp density from low to high changes, the effect of leaching from high to low change.

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CLC: > Biological Sciences > Microbiology
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