Dissertation > Excellent graduate degree dissertation topics show

Effects of Tra1 Gene Mutations on Cell Growth and Gene Expression

Author: LiMin
Tutor: HuangGuoJun
School: Nanchang University
Course: Biochemistry and Molecular Biology
Keywords: Tra1 protein SAGA complex NuA4 complex Ribosomal biosynthesis Pressure-induced gene
CLC: Q343
Type: Master's thesis
Year: 2007
Downloads: 62
Quote: 0
Read: Download Dissertation

Abstract


Growth of eukaryotic cells is closely related with the changes of nutrients and growth factors in the external environment the whole metabolic regulation of the cell is a highly complex and collaborative process conducted by the regulation of gene expression is the most basic level in the whole process . Ribosome as a synthetic protein factory RNA polymerase Ⅰ, Ⅱ, Ⅲ common coordination transcription , which ribosomal RNA gene transcriptional regulation mechanism has been the hot spot of the research of cell growth and cell cycle regulation . Tral protein yeast cell SAGA and NuA4 chromatin remodeling complex common component of protein , these two chromatin remodeling complex study of the role and function of the yeast cells is more thorough . Studies that the Tral protein may be through NuA4 composite participation ribosomal protein gene, the transcription of ribosomal RNA ; through SAGA composite participation pressure inducible gene expression . This topic is the use of the laboratory has been built the Tral 3355 mutation the Tral 3285 3375 double mutant yeast strains , observed their growth under different growth conditions ; through extraction of RNA by Northern blot analysis of different target genes ( His3 His4 RPS7A, Act1, Scr1) expression , found the Tra1 3355 mutation , Tra1 3285 3375 mutant yeast strain in the ribosomal RNA , HIS3, HIS4 , the transcription of the Act1 affected ; unexpectedly discovered that the accumulation of small RNA Tra1 mutant background . The accumulation of these results will be the next step Tra1 mutations on genome-wide expression microarray analysis provides important information . Also SAGA and NuA4 complex of several components of protein plus protein tag (HA3, Myc13) convenience by co-immunoprecipitation and Western detection Tra1 mutant protein SAGA , NuA4 complex integrity . The results of these experiments will help to better understand the role played Tra1 in the eukaryotic ribosome biogenesis and cell growth regulator .

Related Dissertations

  1. Investigation on the Roles of a SAGA Component, Tra1, in Transcriptional Initiation,Q75
  2. Study on the Role of Histone H2B Ubiquitinationin Melosis,Q343
  3. Inhibition of SH-SY5Y Cell Growth by TMEFF2 Gene,Q343
  4. The Change of HES-1、PS-1 and GSK-3β’s Expression in Mouse ESCs after Transfection of Notch1,Q343
  5. Research on the Role of Extracellular Proteinase Inhibitor Expi in Rat Liver Regeneration,Q343
  6. Effects of Gene of Kruppel-Like Factor 4 Klf4 on the Process of Rat Liver Regeneration,Q343
  7. Research on the Role of Sry-box2 Gene SOX 2 in Rats Liver Regeneration,Q343
  8. Effects of Gene of Lipocalin 2 lcn2 on the Process of Rat Liver Regeneration,Q343
  9. The Different Effects of MicroRNA on the Same Target Gene in Normal and Hypoxia Condition of CNE,Q343
  10. Phosphorylation of AP-2α by the CK2 Kinase Results in Repression of AP-2α-mediated Transcriptional Activity,Q343
  11. Caspase Recruitment Domain(CARD)of Swine BC l10 Impacts Its Expression and Cell Cycle,Q343
  12. The Function of Mitogen Activated Protein Kinase(MAPK) in Meiosis Reinitiation of Zebrafish Oocytes,Q343
  13. The Preliminary Study of the Interaction Between Phb2 and clic1,Q343
  14. Expression of Pax6、Pax6 variant in Lens Development and Regeneration from Tadpole of Bufo Raddi Strauch Detected by Western Blot,Q343
  15. Uv-induced DNA Damage Repair Facilitated by Pentose Phospate Pathway in Deinococcus Radiodurans,Q343
  16. A Preliminary Investigation on BRG1’s Role in DNA Damage and Repair,Q343
  17. The Site of Pre-rRNA Processing during the Cell Cycle in Allium Sativum,Q343
  18. PPM1G at the DNA damage checkpoint and spindle checkpoint in functional studies,Q343
  19. Research on the Kinetics of P53 Regulatory Network,Q343
  20. Fast Linkage Analysis Algorithm Research,Q343
  21. The Role of TSC1/2-TOR Signaling Pathway in Drosophila Germline Stem Cell Regulation,Q343

CLC: > Biological Sciences > Genetics > Genetics subdiscipline > Cytogenetic
© 2012 www.DissertationTopic.Net  Mobile