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Isolation and Identification of the Endophytic Fungi from Taxus Chinensis Var Mairei

Author: GuoXiaoJing
Tutor: SongXiaoPing
School: Northwest University of Science and Technology
Course: Clinical Veterinary Medicine
Keywords: Endophytic fungi Taxus chinensis var. Taxol Separate Appraisal
CLC: Q93-33
Type: Master's thesis
Year: 2007
Downloads: 324
Quote: 1
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Paclitaxel the exact many cancers efficacy , but its source has been difficult to resolve drug . Isolation and identification of endophytic fungi of Taxus chinensis the study , the following results were obtained : 1 Isolation and identification of endophytic fungi : 63 endophytic fungi isolated from Taxus chinensis var. , 24 endophytic fungi classification and identification , belonging to 19 species : true ascomycetes and two 2 ; hairy shell genera and Chaetomium globosum 1 , no plush shell is 1 for a newly discovered species ; imperfect fungi Gang 12 of 22 species , including Cladosporium spp three genus Cephalosporium , Penicillium, Trichothecium of the genus Aspergillus , Fusarium , single-stranded spore property of their respective two , Alternaria stem point the case of mildew , Botrytis cinerea , a proposed Penicillium property of their respective three did not produce spores , classified as non- spore taxa . Students within the total separation the imperfect fungi accounted for 91.67% of bacteria Gang , is absolute superiority strains . The culture fluid screening of endophytic fungi produce taxol 2 : No. 14 bacteria isolates ( to Botrytis cinerea ) , No. 16 bacteria (Mycelia sterlia) and 19 bacteria (Mycelia sterlia) extraction, thin layer chromatography , concentrated , Rf values ??were 0.404,0.404,0.401 , consistent with paclitaxel Rf value of the standard , as endophytic fungus of Taxus chinensis taxol production .

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CLC: > Biological Sciences > Microbiology > Microbiology and microbial experiments > Microbiology and microbiology experiments
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