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Research of Mechanism of Phenotypic Plasticity and Optimizing Basic Media on Plantlet Propagated in Vitro

Author: WeiPeng
Tutor: LiuTong
School: Shihezi University
Course: Botany
Keywords: Adapt plasticity Rapid propagation in vitro Rooting medium Arabidopsis Pear
CLC: Q943.1
Type: Master's thesis
Year: 2007
Downloads: 78
Quote: 0
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Abstract


Against vitro propagation plant the successful species number less rooting low growth quality plant growth in vitro totipotency could express, and how to obtain plasticity in response to the process of adaptation to the environment. Closely related to the in vitro plasticity of plant capacity size and growth of quality. Papers in the model plant Arabidopsis (Arabidopsis thaliana) and economic hard rooting the woody plants Pear (Pyrus sinkiangensis Yü) for materials, plant theory and medium-control technology research system to adapt to the establishment of the center, draw: 1. N, P, K nutrient content of the medium, the partially treated Arabidopsis plantlets can smoothly enter the reproductive growth stage, and can bear fruit, suggesting plasticity in response to the nutritional needs of Arabidopsis thaliana under the in vitro conditions with a reasonable nutrition elements composition relative fitness with an active role in improving the plantlets were. With the current research hotspot basic media optimization, phenotypic plasticity mechanism to guide the deployment of medium N, P, K, Ca, Mg five kinds of nutrient elements, rooting rate in more than 90% of the formula S14 : 620 mgl-1 NH4NO3 740 mgl-1KNO3 40 mgl-1MgSO4 0 mgl-1 Ca (NO3) 2 and G1: 1769.4 mgl-1 of NH4NO3 800.9 mgl-1KNO3 496 mgl-1MgSO4 398.6 mgl-1 Ca (NO3) 2 3 plant phenotype in vitro reactions fall under human control the growth of non-nature (such as rooting or proliferation) and plant product \priority rooting under conditions characteristic of its nature, and therefore has a higher rooting rate of plantlets should be a good adaptation of the show. Pear (Pyrus sinkiangensis Yü) demonstrate the analysis of plant nutrients converted into medium suitable for rooting rather than proliferation, and analysis of rooting rate greater than 95% S12 S14 plantlets nutrients converted into the basic medium rooting rate is still greater than 92% of the formulations S12-2, S14-2. Phenotypic plasticity is the basis of in vitro plant growth and development, this study only from the medium optimization level changes of plasticity of the plantlets were nutritional needs. Plants vitro propagation principles, attention to the environmental changes to control the growth of the plantlets will provide more effective theoretical guidance Vitro Propagation enrich and improve phenotypic plasticity.

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CLC: > Biological Sciences > Botany > Plant Cell Genetics > Plant cells in vitro culture
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