Dissertation > Excellent graduate degree dissertation topics show

Study on Polysaccharides Extraction Technology, Bioactivity and High Polysaccharides Germplasm Screening of Oolong Tea

Author: SuYongChang
Tutor: ZhengJinGui;YuanDiShun
School: Fujian Agriculture and Forestry University
Course: Biochemistry and Molecular Biology
Keywords: Oolong tea Polysaccharides Extraction Bioactivity Screening
CLC: TS272
Type: Master's thesis
Year: 2007
Downloads: 199
Quote: 1
Read: Download Dissertation


This study compared and analysed polysaccharides from oolong tea, green tea, black tea and white tea. The extraction technology, biological function of oolong tea were studied systematically, based on which germplams containing high polysaccharide were screened. The results were as follows:1.Optimizing extracion technology of oolong tea polysaccharides. The experiments showed that using hot water ( 80℃) and acetone to extract and precipitate polysaccharides has good results. Through analysing extraction technology by orthogonal test, we obtained the optimal extracton conditions: solid/liquid 1:1, temperature 100℃, time 3h, and three times of actone in volume for polysaccharides precipitation.2.Preliminary Purification of oolong tea polysaccharides. Using Tieguanyin polysaccharides as material, we studied the basic physical and chemical properties, separation and purification of polysaccharides. The results showed that tea polysaccharides mainly were constituted by uronic acid, protein and neutral sugar. After purification,it suggest that polysaccharides were a kind of compounds of polysaccharides and proteins, which had complex ingredients.3.Comparing tea polysaccharides of different preparation treatments. This study used fresh Dangui leaves as materials to undergo different preparation treatments to prepare oolong tea, green tea, black tea and white tea respectively. After hot water extraction,the components were determined and analysed. The results indicated that white tea and green tea had higher polysaccharides availibility and capability of clearing OH free radicals.4.Hypoglycemic effect of oolong tea polysaccharides. Feed polysaccharides for six days to hyperglycemic ICR mouse induced by tetraoxypyrimidine. Fast the mouse for 18 hours and then observe the effects on blood glucose and liver SOD,T-AOC,GSH-PX and MDA. The results indicted that after intaking tea polysaccharides for 6d continuously, the hyperglycemic mice had 21.7% lowered blood sugar level than the control group. and the activity of SOD was enhanced. 5.Screening oolong tea germplasms for high polysaccharides. The experiment selected 52 tea cultivars suitable for oolong tea preparation. The results indicated that there were varitaion among different tea cultivars in polysaccharides content. The cultivar with highest polysaccharides was A01, which polysaccharides content is 4.31%, and it was 1.7, 2 and.2.5 times as high as that of the average level of the 52 cultivar, Tieguanyin and Huangjingui, respectively.

Related Dissertations

  1. Research on Automatic Detection Algorithm for Substructure Distress of Highway Pavement Based on SVM,U418.6
  2. ISAR Imaging Simulation of Space Targets and Target Recognition Based on ISAR Images,TN957.52
  3. Research on Domain Entity Attribute and Event Extraction Technology,TP391.1
  4. Research on Transductive Support Vector Machine and Its Application in Image Retrieval,TP391.41
  5. Research on Feature Extraction and Classification of Tongue Shape and Tooth-Marked Tongue in TCM Tongue Diagnosis,TP391.41
  6. Research on Extraction and Tracking of People’s Opinion,TP391.1
  7. The Research on Paper Currency Classification Method Based on Harr-Like Feature and Minimal Ball Including Samples,TP391.41
  8. Research on Visual Detection and Tracking of Mobile Robots,TP242.62
  9. Study on Phylogenetic Diversity and Bioactivity of Symbiotic Fungi of Coral,R284
  10. Studies on Extraction and Analysis of Fructooligosaccharides in Smallanthus Sonchifolius,TS255.1
  11. Tobacco Diseases Auto-Recognition Research Based on Image Processing Technology,S435.72
  12. Establishment of the Molecular Identifying System of Curvualria and Application in Difficult Species,Q949.32
  13. Research on Nondestructive Detection Technology for External Qualities of Papayas Based-on Vision,S667.9
  14. Study on the Separation and Purification Way of Arctiin from Fructus Arctii and the Effect of Arctiin on the Growth of Mice C2C12 Cells,S853.7
  15. Studies on the Isolation of Oil-Degrading Bacteria with Biosurfactant Production and Their Characteristics,X172
  16. The Supercritical Carbon Dioxide Extraction and Functional Quality Evaluation of Camellia Seed Oil,TS225.16
  17. Study on Extraction,purification and Antioxidant Activity of Wheat Germ Polysaccharides,TS210.1
  18. Study on Growth Monitoring Technique Based on Pixel Un-Mixing Method and HJ Remote Sensing Images in Paddy Rice,S511
  19. Discovery and Mechanism Study of Mitochondrial Mild Uncoupler,R341
  20. Research for Infrared Image Target Identification and Tracking Technology,TP391.41
  21. Study on the Screening and Properties of Degradation Coking Waste Water Advantage Strains,X172

CLC: > Industrial Technology > Light industry,handicrafts > Food Industry > Beverage cold manufacturing industry > Tea
© 2012 www.DissertationTopic.Net  Mobile