|
Objective:Obesity is an important risk factor of insulin resistance and type2 diabetes. In these years, studies find that Adipocyte may secretes manycell factors, visfatin as a cell factor that resemble insulin was be findedin recently. To realize the pathophysiological mechanism of obesity totype 2 diabetes, we assayed plasm levels of visfatin in 50 obese childrenand 30 normal subjects, and investigated the connection visfatin with bodyparameters and other parameters of energy metabolism. We studied theeffect of visfatin in hyperinsulinemia or insulin resistance, and seekedfor a way to prevent from the harm of hyperinsulinemia or insulinresistance in children.Methords:The plasm visfatin concentration was measured in 50 obese childrenand 30 normal subjects by ELISA. We also measured the anthropometricsmeasurement of all subjects. Assessed insulin resistance, fasting glucose,fasting insulin by OGTT in obese children, normal subjects measuredfasting glucose and fasting insulin. Calculated the correlativity ofvisfatin and other parameters.Results:1. Waist circumference, BMI, BF%, WHR, SBP, DBP of obese children werehigher than normal subjects (p＜0.001).2. FPG, FINS, HOMA-R, TG in obese children were higher than normalsubjects(p＜0.001). And, HDL level of obese children was lower than control(p＜0.01). ISI in obese children was lower than normal subjects(p＜0. 001).3. The plasm visfatin concentration of obese children were 32.26 ±6.83ng/ml, those of normal subjects were 25.69±8.05 ng/ml. The plasmvisfatin concentration in obese children were higher than normal subjects(p＜0.001).4. The plasm visfatin concentration was no significantly correlatedwith age, sex; was positively significantly correlated with BMI, BF%, FPG,HOMA-R (r=0.33, r=0.301, r=0.486, r=0.290, p＜0.01);and positivelycorrelated with SBP, DBP, WC, WHR, FINS(r=0.280, r=0.278, r=0.273r=0.242, r=0.221, p＜0.05); and negatively correlated with ISI and HDL(r=-0.269, r=-0.222, p＜0.05); No significantly correlated with TC,TG, LDL.5. Multiple linear regression analysis was used to determinecorrelation coefficient between visfatin and various parameters.Only FPG was entered into the equation, the standard partial coefficientwas 0.486 (p＜0.01),R~2=0.236.Conclusions:1. Waist circumference, BMI, BF%, WHR, SBP, DBP of obese children werehigher than normal subjects. FPG, FINS, HOMA-R, TG, visfatin in obesechildren were higher than normal subjects. ISI and HDL was lower thancontracts.It’s confirmed the idea that higher blood pressure, hyperinsulinemiaor insulin resistance, hypertriglyceridema, and the plasm visfatinconcentration of obese children were higher than normal subjects. Thesesuggested that obese children had the destructive of energy metabolismand a trend development to metabolic syndrome.2. The plasm visfatin concentration was positively significantlycorrelated with BMI, BF%, SBP, DBP, WC, WHR, FPG, HOMA-R; and negativelycorrelated with and HDL and ISI; FPG was most significantly correlated with visfatin.These suggested that the plasm visfatin concentration weresignificantly correlated with the degree of obesity. Those that the plasmvisfatin concentration were correlated with FPG, FINS, HOMA-R, ISI, HDLsuggested that visfatin was significantly correlated with metabolism ofglucose and lipid firstly.
|