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The Experimental Study of β-Carotene to Oxygen Free Radical Expression in Pancreatic Gland Organization of Acute Pancreatitis and to Cytoskeletons’ Affection

Author: WangZuo
Tutor: ZhuoYue；AiYingChun
School: Jiamusi University
Course: Internal Medicine
Keywords: β-Carotene(β-C) acute necrotizing pancreatitis(ANP) Reactive Oxygen Specie(ROS) Actin filaments(F-actin) Cytoskeletons
CLC: R576
Type: Master's thesis
Year: 2007
Downloads: 72
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Abstract

ObjectiveThe multitudinous research indicated that, during the initiation and manifestation of experimental AP, large amounts of ROS are produced. Simultaneously, the antioxidants are consumed thus arouse the contingency harm. The Cytoskeletons are divided into three parts: microtubules, microfilaments (actin filaments—F-actin) and intermediate filaments. Actin, the molecular being 43KDa, is one of the richest proteins in ture nuclear cell, constituted the basic ingredient of microfilaments. It has two kind of forms in the cell, globular-actin,(G-actin), and filamentous-actin (F-actin). Two kind of actin transform mutually according to the cell condition, maintaining a kind of dynamical equilibrium.There are two characteristic pathology and physiological events in AP early time: First is the actin cell skeleton change; second is the rapidexpansion of the intercellular space. At that time the extracellular fluid which contains protein richly inflows, which reflects the common phenomenon of pancreatic gland dropsy. In the gland cell the F-actin arrangement disorder, the basic composition protein change of actin cell skeleton is the reason why the cell skeleton change then disturb enzyme original secretion and cell function. Rapid disruption of the actin cytoskeleton with reduction or loss of cell polarity, enlarged acinar lumina, and loss of the actin network surrounding the lumina; the interstitial space between the polarized epithelial (acinar) cells of the pancreas undergoes an important structural changes.Carotenoids is a kind of important plant pigment, There are so many kinds, includingα- carotene which was well known by people,β- carotene, lycopene and so on. Carotenoids is a kind of compound, constitutes of 40 carbon by the tail-tail connected two 20 carbon unit the molecular parent substances cabon framework, and grows many different compound. Carotenoids’most remarkable structure characteristic is it includes the central molecular region which is constituted by the different number of goal duplet bond or the single bond long chain, forms 1 conjugate system. Most carotenoids has 9 ~ 13 conjugated double bonds, as a result of the C=C duplet bond existence, causes each carotenoids to be possible to have many kinds of isomer. carotenoids has the important biology function, it is a kind of very strong oxidation inhibitor, which can highly quench the single track condition oxygen effectively; They are playing the vital role in the cell membrane, and can mutually affect with other protein members.As a member of the big family of carotenoids,β-C may not only turn into the vitamin A display biology effect in vivo extension, moreover also can eliminate oxygen free radical, quench the single track condition oxygen and the anti- lipin peroxidation biological activity, the organism may obviously improve its anti- state of oxidation. Its mechanism isβ-C may use many duplet bonds and union with the ROS in the cell membrane which in its molecular structure contains, causes ROS to lose activeness, thus may protect the fat membrane to exempt the peroxidation damage. It is the important constitution of organism anti- oxidation defense system Central Africa enzyme system (Vitamin A, E, c, carotene and so on), It isnot only the the essential lipid solubility vitamin which the human body must, moreover its anti- is senile, the anti- oxidation, the anti- tumor, it’s enhancement of organism immunity function had the conclusion.Former domestic and foreign research concentrated onβ-C to its anti- is senile, the anti- oxidation, the anti- tumor, aspect and so on enhanced organism immunity, especiallyβ-C in the experimental model all had the anti- oxidation outside in vivo. But it regarding ANP the endogene anti- oxidation consumption of materials and the oxidation should stir up the damage when the influence function and ANP the pancreatic gland cell skeleton stable influence function aspect study has not reported yet.MethodsMale Wistar rats were divided randomly into three groups: group A、group B and group C. Group A was injected 5% sodium taurocholate. Group B receivedβ- C therapy, and both groups served as experimental groups, while group C as control group. Rats in three groups were killed at30`、1h、3h、6h、12h.The levels of malonyl dialdehyde (MDA)、total superoxide dismutase (SOD)、glutathione (GSH)、serum amylase (SAM) and ATP were measured . Histopathology was observed.Immunostained F-actin with rhodamine-phalloidin was analyzed using confocal laser scanning system..ResultsLevels of SAM increased in group A and group B, with related pathological changes of ANP .Levels of GSH、SOD in group A decreased markely during pancreatiti(P<0.05),however MDA increased significantly (P<0.05) .The levels of GSH、SOD、ATP in group B were higher than those in group A (P<0.05).The level of MDA was lower than that in group A (P<0.05) . And the histopathologic damage was attenuated to a certain extent. ANP resulted in early disruption of the cytoskeleton with dramatic changes with a loss of F-actin. Administration ofβ-C moderated the damage of actin-cytoskeleton.ConclusionANP resulted in pancreatic gland organization endogene anti- oxidation matter remarkable drop, lipin peroxidation increase. It has the obvious oxidation supposed to stir up the damage,β-C element may obviously reduce the ANP rat pancreatic gland cell damage as the oxygen free radical elimination medicinal preparation. We demonstrated that the cytoskeleton alterations are a common feature of AP, and administration ofβ-C can protect actin cytoskeleton during ANP

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CLC: > Medicine, health > Internal Medicine > Digestive and abdominal diseases > Pancreatic diseases
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