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Test box plant cultivation in the Northeast Agricultural University in 2005-2006, test varieties the Heinong 37 and \Different fertilization period set in the same nitrogen level in 2005, set three nitrogen levels in 2006, and set a different period of fertilization. In major growth period measured root nodule dry weight, nitrogenase activity and plant dry matter and nutrient content of various fertilization on soybean nodule nitrogenase activity, dry matter accumulation, nutrient accumulation and production. Settings of a nitrogen levels (N75kg/ha) 2005 processing, N25, N30 45 (R 3 ), N30 45 (the R 4 ), N75, and do blank control. The results show that, in VE-R 3 stage, N75 basal fertilizer nitrogen overuse, significantly inhibited the growth of roots and nodules of students development, N25 × 3, and N30 45 by reducing the amount of nitrogen in the basal fertilizer reduce nitrogen fertilization on the inhibition of root and R 3 period nodule, root dry weight were higher than N0 treatment, nitrogen application on the inhibition of nitrogenase activity in R 3 < / sub> of apparent among treatments N0 N30 45 (the R 4 )> N25 × 3> N30 45 (the R 3 )> N75. R 4 -R 6 of nitrogen fertilizer on root inhibition reduced, all N treatments root nodule dry weight increased significantly to R 5 of the maximum, to N topdressing significantly increased the accumulation of the roots of this period, of which R 4 dressing is best, R 6 Phase dry weight determination results show that, top-dressing effectively delaying the the late roots of the recession, its root nodules, root dry weight of different degrees higher than the N75 treatment. The nitrogenase activity results showed that the N25 × N30-45 (R 3 ), N30 45 (R 4 ) makes three topdressing nitrogen fixation peak shift, there in R 5 of N75 nitrogenase activity peak in R 4 of the R 6 of each treatment nitrogenase activity N30 45 (R 4 )> N30 45 (R 3 )> N25 × 3> N75> N0. Correlation analysis showed nitrogenase activity and early nitrogen accumulation of a negative correlation, R 5 nitrogenase activity was a significant positive correlation with yield. The results showed that the dry matter accumulation and nutrient accumulation N25 × N30 45 (R 3 ), N30 45 (the R 4 ) in the reproductive growth phase are given a higher nitrogen accumulation and dry matter accumulation, which N30 45 (R 4 ) dressing best, nitrogen accumulation amounted to 5.50 g / box, dry matter accumulation capacity of 199.38g / box. The amount of each growth stage of accumulation results show that the Effects of nitrogen fertilizer, slow R 4 -R 6 root recession, increase the period of dry matter accumulation and nutrient accumulation, effectively promote the pod in nitrogen accumulation and transfer of dry matter in the pod, from the dressing effect, R 4 > R 3 > R 2 R 5 . Test species yield monitoring results show that the output of each treatment N30 45 (R 4 )> N30 45 (the R 3 )> N75> N25 × 3> N0, N30 45 ( R 4 ) compared to N75 yield 8.85% the N30 45 (R 3 ) yield to 5.35%, N25 x 3 stimulation effect is not obvious, the the yield components factor analysis results show that, production increase by increasing the number of strains pod and strains grains, R 5 dressing to improve kernel weight better. By correlation analysis, the-R production and R 4 6 nutrient accumulation and dry matter accumulation was highly significant positive correlation N75 level production and VE dry matter accumulation was negative correlation. N25, N50, N75 three nitrogen levels set in 2005 on an experimental basis in 2006, and the creation of different levels of N50 and N75 fertilization period. The results showed that the N75 and N50 compared to the excessive application of nitrogen fertilizer, and can not obtain high yield, N25 × 3 by adjusting the application time, improve the utilization of fertilizer. From the dry matter and nutrient accumulation measurement results, N25 x 3> N75, N50 level, N25 × 2 (R 4 )> N25 × 2 (R 3 ) > N25 × 2 (R 1 ). Measurement results from the production, N25 (R 4 )> the the N25 × 3 N25 × 2 (R 3 ) × N25 × 2 (R 1 )> N50> N75> N25, and N50, N25 × 2 (R 4 ) production increased by 23.1% to reach a significant level of 5%, N25 × 2 (R < / sub>) production increased by 8.35%, N25 × 2 (R 1 ) yield increased by 1.22%. Compared with N75, N25 × 3 production increased by 18.17% to the 5% significance level. The two-year results show that the basic soil to determine the appropriate amount of nitrogen to reduce the amount of basal fertilizer nitrogen, to promote early root growth, increased post-nutrient accumulation and dry matter accumulation have a positive effect, and increase the utilization rate of fertilizer, reducing excessive nitrogen application on the environment caused by pollution.
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