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This test uses diluted drops of species of 10 normal flora of the the healthy lactating system in 30 healthy female cattle qualitative and quantitative detection. To determine the types and quantities of the normal flora of cattle lactation system parts, and analysis of the distribution of the normal flora in the cattle the lactating system four parts. Since the 1960s, the domestic animal gut, the normal flora of the respiratory tract, reproductive tract, and other channel systems have certain reports, has not been reported in the normal flora of the mammalian lactation system. Selected had lactation feeding calves healthy female cattle, by its appearance of, breast milk samples health status check identified as healthy cattle, healthy breast can be used for the detection of the normal flora of the lactation system. Aseptically from four parts of the breast, cattle (nipple, milk pool, lactiferous duct and acinar) bacteria cotton swab dipped into a sample 0.1ml, and its diluted, the dilution was inoculated into the selective and non- selective medium, the appropriate medium for the aerobic or anaerobic culture. Lactobacilli (Lactobacillus) coryneform (genus Corynebacterium), Staphylococcus aureus (Staphylococcus), Bacillus (Bacillus), bifidobacterium (Bifidobacterium), Enterobacteriaceae (Escherichia), enterococci (Enterococcus), Bacteroides (Bacteriodes), Eubacterium (Clostridium) and Clostridium (Eubacterium) 10, the normal flora of the qualitative and quantitative analysis. Observed characteristics of colonies on each medium, typical colonies were picked to microscopy and done in pure culture, pure culture necessary biochemical tests, including nitrate reduction test, gelatin liquefaction test, indole test, producing H 2 S test, contact of the enzyme test, urease test, esculin hydrolysis test, glucose, lactose, maltose, sucrose fermentation test. Based on the above characteristics to identify flora. The test results as follows: there are six kinds of flora is detected, respectively coryneform (genus Corynebacterium), (Lactobacillus), Lactobacillus, Staphylococcus aureus (Staphylococcus), Bacillus (Bacillus), bifidobacterium (Bifidobacterium) and Enterobacteriaceae (Escherichia ). Coryneform bacteria (Corynebacterium) in the milk pool up to 0.1ml samples, containing an average of 680 CFU / ml, containing an average of 203 CFU / ml in the acinar least 0.1ml samples; lactobacilli (Lactobacillus) in most milk pool, 0.1ml samples in containing an average of 882 CFU / ml, in the acinar least 0.1ml samples, containing an average of 117 CFU / ml; aureus (Staphylococcus) in the nipple up to 0.1ml samples, containing an average of 372 CFU / ml, in the acinar least 0.1 mL sample containing an average of 36 CFU / ml; maximum of Bacillus (Bacillus) in the nipple, 0.1ml samples containing an average of 423 CFU / ml, in the acinar not isolated; Bifidobacterium (Bifidobacterium) in the lactiferous duct maximum 0.1 mL sample containing an average of 203 CFU / ml, at least in the nipple, the the 0.1ml sample containing an average of 22 CFU / ml; separated only in a cattle nipple Enterobacteriaceae (Escherichia), 0.1ml samples containing only 120 CFU / ml. The number of cattle lactation system normal flora are much less than the other channel systems. Cattle lactation systems are dominant Corynebacterium (Corynebacterium) and Lactobacillus (Lactobacillus). The nipple are dominant in cattle lactation system Corynebacterium (Corynebacterium), Bacillus (Bacillus) and Staphylococcus aureus (Staphylococcus); the milk pool the milk ducts and acinar advantage flora are Corynebacterium (Corynebacterium) Lactobacilli (Lactobacillus). Detected, the same head of cattle lactation system four milk flora species and the number of the difference was not significant, so you can select any one to take samples in four breast area. Flora in the distribution of the various parts of the lactation system: Corynebacterium (Corynebacterium) and the number of lactobacilli (Lactobacillus) milk pool up, both in the other three parts by the number at the order of the milk ducts, nipple and acini. Bacillus (Bacillus) and the number of staphylococci (Staphylococcus) nipple up. Bacillus (Bacillus) inferior to the nipple in the milk pool and the number of milk ducts in the acinar not isolated; staphylococci (Staphylococcus) in the other three parts by the number at the order of the milk ducts, milk pool and glandular bubble. The number of bifidobacteria (Bifidobacterium) in the milk ducts up, and then turn to the milk pool, acinar and nipple. The test results show that the distribution pattern of the normal flora of cattle lactation is consistent with the other blind end of the system, such as the respiratory system, reproductive system. More close to the depths of the blind side, the various flora fewer the number of or in the form of a sterile state. Speculated cattle lactation system is sterile before in lactation, lactation after the newborn animal suck generate negative pressure and milk rich in nutrients for normal flora colonization conditions; acinar sterile or bacteria at least, but because of the milk accumulation and backflow, which could affect the test results; lactating cows at different lactation, breast flora will vary. Another characteristic of the normal flora of cattle lactation is a small number of non-astronomical, much less than other systems. Cow mastitis is a common disease of cows, the the world dairy cow industry one of the most important hazards. The cause is more complex, causing mastitis pathogens are also a lot of prevention difficult. If from the point of view of Microecology prevention and control, and may well be a way. The test for the cow lactation Microecology some information has certain physiological and pathological significance.
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