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Screening of Pathogenesis Related Proteins Mediated by Rice Disease Resistance Gene Xa21

Author: WuPengCheng
Tutor: LiuGuoZhen
School: Agricultural University of Hebei
Course: Biochemistry and Molecular Biology
Keywords: Rice Bacterial blight Resistance gene Two-dimensional electrophoresis Mass Spectrometry Antibody
CLC: S511
Type: Master's thesis
Year: 2008
Downloads: 113
Quote: 0
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Rice is the most important food crop in the world , is one of the important food crops . Rice bacterial blight caused by Xanthomonas oryzae pv.oryzae (Xoo) , is a serious bacterial disease . Rice suffered bacterial blight infection severely reduced , or even crops, disease resistance gene -mediated molecular mechanism of rice production is of great significance . Xa21 is the first cloned rice disease resistance gene , but also monocot plants first cloned resistance genes , with broad-spectrum resistance to bacterial blight , the gene encodes a receptor-like kinase protein . In this experiment , we used two-dimensional electrophoresis combined with mass spectrometry to find some differences in protein expression . Transgenic rice varieties 4021 materials, Xanthomonas oryzae after inoculation of leaf protein , separation of proteins by two-dimensional electrophoresis , mass spectrometry analysis will analyze the protein spots were found after the 48 candidate proteins according to their different functions can be divided into for the nine categories . Combining the preliminary results of microarray analysis AP2 domain the 26S proteasomeregulatory Particle triple- A ATPase subunit 6 ( Rpt6 ) of the protein expression of these four genes of the glutathione S - transferase ( GST ) and 2-Cys peroxiredoxin (2Cprx) may be provided with anti- Xa21 related disease . These four protein expression in a prokaryotic expression system and purified antibody was prepared by the Western analysis showed that the GST protein expression level in 5 days of inoculation , at different time points after inoculation , no visible changes in expression of 2CPRX protein ; increased. Antibody preparation for the next step of the analysis provides a biochemical basis .

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