Dissertation > Excellent graduate degree dissertation topics show

Screening of Pathogenesis Related Proteins Mediated by Rice Disease Resistance Gene Xa21

Author: WuPengCheng
Tutor: LiuGuoZhen
School: Agricultural University of Hebei
Course: Biochemistry and Molecular Biology
Keywords: Rice Bacterial blight Resistance gene Two-dimensional electrophoresis Mass Spectrometry Antibody
CLC: S511
Type: Master's thesis
Year: 2008
Downloads: 113
Quote: 0
Read: Download Dissertation

Abstract


Rice is the most important food crop in the world , is one of the important food crops . Rice bacterial blight caused by Xanthomonas oryzae pv.oryzae (Xoo) , is a serious bacterial disease . Rice suffered bacterial blight infection severely reduced , or even crops, disease resistance gene -mediated molecular mechanism of rice production is of great significance . Xa21 is the first cloned rice disease resistance gene , but also monocot plants first cloned resistance genes , with broad-spectrum resistance to bacterial blight , the gene encodes a receptor-like kinase protein . In this experiment , we used two-dimensional electrophoresis combined with mass spectrometry to find some differences in protein expression . Transgenic rice varieties 4021 materials, Xanthomonas oryzae after inoculation of leaf protein , separation of proteins by two-dimensional electrophoresis , mass spectrometry analysis will analyze the protein spots were found after the 48 candidate proteins according to their different functions can be divided into for the nine categories . Combining the preliminary results of microarray analysis AP2 domain the 26S proteasomeregulatory Particle triple- A ATPase subunit 6 ( Rpt6 ) of the protein expression of these four genes of the glutathione S - transferase ( GST ) and 2-Cys peroxiredoxin (2Cprx) may be provided with anti- Xa21 related disease . These four protein expression in a prokaryotic expression system and purified antibody was prepared by the Western analysis showed that the GST protein expression level in 5 days of inoculation , at different time points after inoculation , no visible changes in expression of 2CPRX protein ; increased. Antibody preparation for the next step of the analysis provides a biochemical basis .

Related Dissertations

  1. Study on Rice-Frog and Melon-Frog-fish Ecological Cultivation and Breeding Mode and the Nutrient Balance,S-0
  2. Study on Bispecific Monoclonal Antibody to Pesticide Imidacloprid and Parathion-Methyl,S482.2
  3. Discovery and Biological Activities of Natural Michael Addition Acceptors,R284
  4. Metabonomics Study on the Usnic Acid and Erthromycin Estolate-induced Liver Toxicity by GC-MS,R965
  5. Establishment of ELISA for Detecting Cd-MT of Pteria Penguin and Preliminary Study of ELISA-kit,X835
  6. Study of Triptolide Induced Hepato-toxicology by MS-based Metabonomics Approaches,R285
  7. Expression and Purification of Influenza a Virus M2 Protein and Detection of Its Antigenicity,R392
  8. SCXC corporate culture construction,F270
  9. Monitoring of Insecticide Resistance and Resistance Risk Assessment for JS118 in the Rice Stem Borer, Chilo Suppressalis (Walker),S435.112.1
  10. Arthropod Diversity in Non-Crop Habitats at Margins of Paddy Fields of Different Types,S435.112
  11. Detection of the Resistance in Xanthomonas Oryzae PV. Oryzae and X. Oryzae PV. Oryzicola to Two Bactericides and the Assentment of Resistance Risk in Vitro,S435.111.4
  12. An Approach for Identifying a Plant Resistance Gene Based on the Random Forest,Q943
  13. Study on Application of Red Yeast Rice in Fermented Sausage,TS251.65
  14. The Research on Improvement of Rice Noodles Quality,TS213.3
  15. Studies on the Properties of Starch from Germinated Polished-rice-with-germ,TS235.1
  16. The Research on the Comprehensive Modification Technology of Rice Protein Enzymatichydrolysis and Graft Copolymerization,TS201.2
  17. The Rice OsNAR2.1 Participates the Regulating Root Growth by Nitatre,S511
  18. Research on Feed Safety of Transgenic Rice in Broiler,S831.5
  19. Cellular and Proteomic Analyses of Endocytosis Mechanism Involved in Pollen-tube Growth in Nicotiana Tabacum,Q942
  20. Soil Eoclogical Risk Evaluation of Transgenic Bt Rice Through Nematode Community Analysis,S154.1
  21. Genetic Analysis and Molecular Mapping of QTL for Rice Black-Streaked Dwarf Disease Resistance in Rice,S511

CLC: > Agricultural Sciences > Crop > Cereal crops > Rice
© 2012 www.DissertationTopic.Net  Mobile