Dissertation > Excellent graduate degree dissertation topics show

Screening Small Anti-leukemia Compounds Based on EDAG

Author: DiZuo
Tutor: WangSiYing
School: Anhui Medical University,
Course: Pathology and Pathophysiology
Keywords: EDAG Transcriptional activation The dual luciferase Report system Drug screening
CLC: R733.7
Type: Master's thesis
Year: 2007
Downloads: 19
Quote: 0
Read: Download Dissertation

Abstract


Objective: To establish based EDAG ( erythroid developmental gene erythoid develop associated gene ) transcriptional activity of new small molecule compounds in vitro screening model and use the model to screen small molecule compound libraries , expect to quickly and efficiently find the pilot molecules with potential anti-leukemia . Methods: The experimental method of the luciferase reporter gene to The EDAG the transcriptional activation quantify establish the model of large-scale screening of active small molecule compounds and screening of small molecule compound libraries (about 1000 ) . Tetrazolium MTS colorimetric , 3H-TdR incorporation assay , flow cytometry screening active compounds to high expression of endogenous the EDAG the leukemia cell proliferation , apoptosis and differentiation ; application of signal transduction pathways specific inhibitor (U0126, CalphostinC, LY294002) the influence of the test compound on the EDAG luciferase reporter gene system , and further verified by Western blot , and thus the mechanism of the candidate active compound obtained in preliminary discussion . Results: pM-EDAG expression vector was successfully constructed , and the establishment of a high-throughput screening model , the application of the model obtained by screening of candidate active compounds 2G2 and 2F10 . 2G2 inhibition of EDAG transcriptional activation of specific inhibition of K562 cell proliferation , promote apoptosis , but no significant effect on their differentiation ; 2F10 enhance transcriptional activation activity of EDAG promote megakaryocytic differentiation of K562 cells were , did not significantly inhibit proliferation and promote apoptosis , MAPK signal transduction pathway inhibitor U0126 2F10 transcriptional activation effect specific inhibition . Conclusion: This study successfully established a high - throughput screening of the EDAG transcription activity influential model of the active compounds , the preliminary study of the function and mechanism of action of the active compounds . Lead compounds for further reconstructive , may provide a new target for the treatment of leukemia .

Related Dissertations

  1. PPARs established based on human cells that target drug screening platform,R965.1
  2. Initial Exploration of ZNFD Biological Functions and ZBTB9 Cloning and Expression of Mouse Zinc Finger Protein,Q78
  3. Effect of TLR4 Promoter Polymorphisms on Transcription Activity in Neonates,R346
  4. Establishment of Anti-HCV Compounds Screening System and Its Preliminary Application,R978.1
  5. A strain I03A-09005 Purification , structural identification and biological activity of two , targeting the cell wall of Mycobacterium tuberculosis drug screening studies,R96
  6. Construction of the Primary Medicine Screening Technique Based on Human Promoter of CXCR4,R96
  7. The Study and Application of Enzyme Biochromatography for the Screening of Aminopeptidase N Inhibitors,R730.2
  8. Experimental study of anti- metabolic syndrome drug screening and DA anti- metabolic syndrome, the role and mechanism of action,R589
  9. Establishment and Evaluation of a Murine Nephritis Model with Chronic Graft-versus-host Disease,R392
  10. Induction of GCH1 Expression by LPS,R341
  11. Construction of Screening System of Human Cu/Zn Superoxide Dismutase Promoter Up-regulating Peptide,R341
  12. Localization of α1B-adrenoceptor Using PEG-Prazosin-QDs and Developing of a Drug Screeing Model,R96
  13. Establishment of LDLR Agonist Model & Its Application in Study of Cholesterol-Lowering Effect of Dark Tea,R285
  14. Establishment of the Dual-Luciferase Reporter System for Screening Anti-Tumor Medicine Base on the P21 Pathway,R96
  15. Initial Exploration of the Function of ZNFD-a C2H2-type Zinc Finger Protein,Q78
  16. Studies on Biological Function of the Cotton MYB Transcription Factor GhMS3,S562
  17. The Cloning and Molecular Characterization of Tanac Genes in Wheat,S512.1
  18. Grape anthracnose occurrence and control of drug screening,S436.631
  19. Establishment of a High Throughput Screening System of Polypeptide Drug Based on Cell Penetrating Peptide Technology,R965.1
  20. Cloning and expression of Mycobacterium tuberculosis DNA gyrase 44 subunit B inhibitors Isolation and purification of,R378
  21. Study on Submerged Culture of Rare Edible and Medical Fungi and Bioactivities of Their Products,R285

CLC: > Medicine, health > Oncology > Hematopoietic and lymphoid neoplasms > Leukemia
© 2012 www.DissertationTopic.Net  Mobile