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Eperythrozoon (Eperythrozoon) parasites on the host erythrocyte surface or separated from the plasma, tissue fluid, and cerebrospinal fluid. Can infect goats, cattle, pigs, horses, sheep, mice, dogs, blue foxes, chickens and other animals and even humans. But For Eperythrozoon, there has been significant differences. The due to characteristics of protozoa Eperythrozoon, initial the Eperythrozoon belongs protozoa; Thereafter, he found its purpose characteristics of Rickettsia again it is classified as Rickettsia project. Some scholars at home and abroad in recent years, using molecular biology techniques the classification of Eperythrozoon, found 16S rDNA Anaplasma relatively distant homology, with Mycoplasma head, Mycoplasma Section blood Barton's body closer homology, and suggested that it be re-classified as Mycoplasma Branch. Bergey's Manual of Systematic Bacteriology, \With the completion of the sequencing of the human genome, the post-genomic era, bioinformatics has been widely applied in the field of life science research. Especially for bioinformatics analysis of prokaryotic 16S rDNA sequences can be used as an effective complement to microbial phenotype, for the study of microbial systematics. In this study, the use of network database rich resources, 16S rDNA sequences of representative strains from Eperythrozoon looking for new molecular markers of the classification of Eperythrozoon and eperythrozoonosis of molecular diagnostics research. Downloaded from the NCBI GenBank public database Eperythrozoon animals such as pigs, cattle, sheep, dogs, cats, rats, and in connection with similar biological representative strains of 16S rDNA sequences (as of May 2008), such as: blood Barton's body and pathogenic Mycoplasma, Rickettsia, use of biological informatics method of homology analysis. The results show that the homology of pigs, cattle, sheep, dogs, cats, rats and other animals Eperythrozoon blood Barton's body is relatively close, form three parallel branches with some common pathogenic Mycoplasma the rickettsial homology is relatively far. 3 suis (M.suis) and 3 of cattle Wen's Eperythrozoon (M.wenyonii), on behalf of the 16S rDNA sequences of strains hypervariable region elect for some base multiple sequence alignment analysis Variance larger fragment (40bp) (after analysis of the fragment located in the hypervariable region of 16S rDNA v9). Further secondary structure analysis revealed significant difference between the two kinds of kinds of conformation is almost no difference in the M.suis species, the obvious differences M.wenyonii species, Description the various body 16SrDNA in pigs, cattle suis The secondary structure of the similarities and differences between hypervariable region is not entirely dependent on the composition of the primary structure, i.e., the same sequence in the secondary structure, a structure may be the same and may also be different. (Or subtle differences may be caused by secondary structure of the primary structure of a difference, the difference may not occur.) Therefore, the secondary structure may be embodied a structure can not be the variation of performance. Several attached to red blood cell body and closely related species of blood Barton's body of 16S rDNA RFLP simulation analysis found that, by restriction mapping analysis, filtering out Alu Ⅰ Dde Ⅰ with Hae Ⅲ in Hha Ⅰ, Hinf Ⅰ, of Hpa Ⅱ (Msp Ⅰ) Rsa I, Xbal I endonuclease, through a combination of one or several digested with type Basic can be used to conduct a comparative analysis of the biological species-level taxonomic units. 8 endonuclease the analog digested by these kinds of 16S rDNA (greater than 100bp), screened 16S rDNA RFLP candidate molecular markers to distinguish Eperythrozoon blood Barton's body. 16S rDNA sequences according the NCBI database Eperythrozoon representatives strain, design Eperythrozoon, genus-specific primers, respectively, from the microscopic examination was the positive Eperythrozoon in pig's blood and chicken blood amplified by the two treaties 700bp purpose of The strips, the strip identified by cloning and sequencing purposes. By optimizing the conditions of Mg2 concentration, annealing temperature and number of cycles of the PCR reaction, a the genus Eperythrozoon specific PCR diagnostics. Optimize the reaction conditions, PCR, a specific test, sensitivity test results prove that the method of high specificity and sensitivity, can be used for diagnosis and molecular epidemiological investigation eperythrozoonosis. In this study, all districts and counties of Chongqing chicken farming methods and different geographical locations and terrain features, with reference to 2006, all districts and counties of Chongqing chicken farming, choose the large-scale chicken Rongchang, Dazu, Yongchuan, Jiangjin, Aikawa, Bishan 2442 Yu anticoagulant, longevity areas and has a typical mountain farming methods the Wulong Wanzhou, Yunyang, the city population, Qianjiang, Xiushan, etc. 13 districts (counties) as the sampling points were collected chicken parts. The Giemsa stained microscopic examination, full blood pressure slides examined universal primers PCR, specific PCR was used to detect the positive rate of 9.986%, 3.398%, 0.555%, and 0. The results show that: Chongqing, yet no real sense of chickens infected with red body cases, microscopic examination detected a higher false positive PCR results as a basis for accurate diagnosis.
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