Dissertation > Excellent graduate degree dissertation topics show

Molecular Identification of Begomoviruses Infecting Malvastrum Coromandelianum in Yunnan Province

Author: LiuPei
Tutor: ZhouXuePing；XieYan
School: Zhejiang University
Course: Biochemistry and Molecular Biology
Keywords: Malvastrum coromandelianum begomovirus DNA-A DNAβ mixed infection
CLC: S432.41
Type: Master's thesis
Year: 2008
Downloads: 43
Quote: 1
Read: Download Dissertation

Abstract


Geminiviruses have been reported infecting crops and weeds such as tobacco, tomato,Malvastrum coromandelianum and Siegesbeckia orientalis and have caused significant yield losses in Yunnan Province.Weeds are important alternative hosts for geminiviruses.So research works were carried out to identify viruses infecting Malvastrum coromandelianum plants in Yunnan province.Isolate Y278 was collected from Malvastrum coromandelianum in Baoshan, Yunnan.The complete DNA-A sequence of Y278 has the highest sequence identity (87.0%)with that of Malvastrum yellow vein virus.So it is a distinct begomovirus,for which the name Malvastrum yellow vein Baoshan virus(MYVBSV)is proposed. Sequence comparison indicates that Y278 probably have arisen from recombination between Malvastrum yellow vein viru(MYVV)and viruses related with Sida leaf curl virus(SiLCV).Tomato yellow leaf curl China virus(TYLCCNV)DNA-A and associated DNAβmolecules were isolated from three Malvastrum coromandelianum samples in Yunnan. Y278 DNA-A and DNAβfrom Y277,Y278 and Y281 were sequenced.Comparisons with other begomoviruses showed that Y278 DNA-A is most closely related to TYLCCNV-[Y264]with 100%nucleotide sequence identity.The three associated DNAβshares the highest nucleotide sequence identity(97.0～99.7%)with TYLCCNV DNAβ.So Malvastrum coromandelianum is an alternative host of TYLCCNV.The DNA-A sequence of Y277 from M.coromandelianum sample collected in Baoshan,Yunnan were determined.Comparison of nucleotide sequences indicate that Y277 has the highest nucleotide sequence identity(99.6%)with that of MYNYNV. Infectious clone of DNA-A of Y277 was constructed.Y277 DNA-A alone or together with MYVYNV DNAβcould systemically infect Nicotiana benthamiana plants,so DNAβmolecule is not necessary for symptom induction of Y277 DNA-A,but DNAβcan intensify symptom severity and increase DNA-A accumulation in plants. Mixed infections were tested with specific primers among 22 Malvastrum coromandelianum samples collected from several areas of Yunnan province.The results showed that most samples were infected by no less than two kinds of begomoviruses and begomovirus/DNAβcomplex occur commonly in fields. MYVBSV and TYLCCNV were found in many Malvastrum coromandelianum samples collected from different areas of Yunnan province,indicating these viruses occur widely in fields.

Related Dissertations

  1. Growth Rate and Growth Model of the F5 Selected Lines for Four Shell Colors of Pinctada Martensii （Dunker） Cultured in Deepwater Area of Beibu Bay in Zhanjiang and Their Genetic Diversity Analysis Using SSR Molecular Markers,S968.31
  2. Treatment Efficiency of Entecavir and TACE in Patients with Primary Heptatic Carcinoma of the HBV Copying,R735.7
  3. Establishment of the Molecular Identifying System of Curvualria and Application in Difficult Species,Q949.32
  4. Detection of Exogenous Recombinan DNA of Transgenic Rice and Its Rice Product,S511
  5. Primary Studies on Transcriptome and Population Genetics of An Invasive Weed Mikania Micrantha,S451
  6. Investigation of Cereal Cyst Nematode on Wheat in Jiangsu Province and the Sequence Analysis of rDNA-Its Region from Jiangsu Populations,S435.121
  7. Development and Identification of Structural Variations Involving Chromosome 1J and 5J of Th. Bessarabicum L(?)ve,S512.1
  8. Studies on Extracting Mitochondrial DNA and Gene Expressions in Brassica Napus L,S565.4
  9. Development and Identification of a Triticum Aestivum-Thinopyrum Bessarabicum Chromosome Translocation Line and Analysis on Its Genetic Effect,S512.1
  10. Studies on the Mechanism and Recovery Measures of Hyperhydricity of Pyrus Calleryana Decne in Vitro,S661.2
  11. In Vitro Rapid Propagation System of Peach Yu Dwarfing Rootstock No.1 and Optimization of Its SSR-PCR System,S662.1
  12. Effects of 5-Aza-2-deoxycytidine on DNA Methylation and Expression of p16 and MGMT Gene in Lung Cancer Cell Line SPC-A-1,R734.2
  13. Methylation of p16 Gene in Plasma and Tissues from Non-small Cell Lung Cancer Patients, Demethylation and the Biological Behavior of Lung Cancer Cell and Transcription of p16 Gene,R734.2
  14. DNA Prime/Protein Boost of Japanse Edcephalitis Virus NS1 Protein to Improve Immune Response in Mice,R392
  15. Analysis of DNA Barcoding on Partial Indigenous Duck Breeds,S834
  16. Molecular Phylogeny of Rhus Gall Aphids (Hemiptera: Aphididae: Pemphiginae) Based on DNA Sequences,S899.4
  17. The Preliminary Study of the pcDNA3.1(+)-SjP14 Nanometer Microballoon-DNA Vaccines,R392.1
  18. Effect of SO2 Exposure on DNA Methylation Polymorphism in Arabiadopsis Thaliana,Q943
  19. Synthesis of 5-Cl-salicylate-Chromium(Ⅲ) Complexes, and Properties and Their Lipid-lowering Activity,O627.63
  20. The Small Organic Molecules Targeted Human Telomeric G-quadruplex DNA: Design, Synthesis and Binding Properties,TQ460.1
  21. Novel Imidazophenanthroline Derivatives: Design, Synthesis and Evaluation of Biological Activity,TQ460.1

CLC: > Agricultural Sciences > Plant Protection > Pest and Disease Control > Plant diseases and their prevention > Transgression ( pass ) an infectious agent harmful > Virus
© 2012 www.DissertationTopic.Net  Mobile