Dissertation > Excellent graduate degree dissertation topics show

Determination and Analysis of 1-deoxynojirimycin in Mulberry and Silkworm

Author: ZhuJian
Tutor: LiJing
School: Southwestern University
Course: Biochemistry and Molecular Biology
Keywords: 1 - deoxynojirimycin Silkworm Mulberry leaves Content determination Enrichment patterns
CLC: R284
Type: Master's thesis
Year: 2008
Downloads: 276
Quote: 2
Read: Download Dissertation

Abstract

1 - deoxynojirimycin (DNJ) and its derivatives is an effective α-glucosidase inhibitors, can be used in the treatment of diseases such as diabetes, obesity, infection. A variety of plants and microbes have been found with the characteristics of the synthesis and accumulation of DNJ, which the highest content in mulberry leaves. Eat mulberry, silkworm life of its body the DNJ content is much higher than mulberry leaves. By the limitations of the test method, the current system for DNJ content in mulberry leaves and silkworm body changes or enrichment patterns not many studies. Established by reversed-phase high performance liquid chromatography - UV detection method (RP-HPLC-UV) determination of DNJ, using this method to study the variation DNJ content in mulberry leaves, and silkworm DNJ enrichment. The plant alkaloid plants in the process of co-evolution with insects formed a large class of secondary metabolites defensive, DNJ is an alkaloid content of mulberry leaves, but the insects outside the silkworm DNJ The role of research is also less. The papers in these areas, a number of studies, the main results are as follows: 1.DNJ determination method. Solvent extraction silkworm powder and mulberry leaf powder DNJ 9 - fluorenyl methyl methyl chloroformate (FMOC-Cl) as derivatization reagent, 0.4mol / L potassium borate buffer, pH = 8.5 to 0.05 mol / L HCl derivatization reaction of DNJ-FMOC complex, reversed-phase high performance liquid chromatography with UV absorption - UV detection method for the determination of DNJ-FMOC. The results show that: the chromatographic separation of the derivative product DNJ-FMOC peak area of ??DNJ concentration was positively correlated with the recovery rate was 94.98%, precision experiments, reproducible experiments, stability testing, recovery experiment shows that the method is reliable DNJ content measured silkworm and mulberry leaves, and related pharmaceutical health care products can be used as an effective method. 2 by reversed-phase high-performance liquid chromatography (RP-HPLC-UV) method for the determination of the wild silkworm, between different strains of the silkworm, 5th instar larvae of different tissues and organs, and silkworm larvae the fifth instar different growth period 1 - deoxynojirimycin (DNJ) content. The results show that: the wild silkworm Bombyx mori silkworm different strains DNJ content very significant difference. Highest DNJ content in the test sample, the wild silkworm silkworm powder, 0.4278%, followed by oil silkworm yellow blood silkworm minimum, 0.2205%; DNJ content in the silkworm different organization there are significant differences in the highest content of silkworm blood meal, followed by midgut, body wall and fat body. Silk gland is almost undetectable DNJ; DNJ content silkworms silkworm development was a significant change four days of age the highest content of 0.329%. After 5 days to decline rapidly. From the point of view of the use of hypoglycemic ingredients DNJ to produce full silkworm powder, oil silkworm larvae of fifth instar 3 days -4 days is better. 3. Cultivation Sanglu Sang, white mulberry, bilberry, three categories and nine varieties, Wild Sang Mengsang, ghost Sang, Wah Sang, long fruit mulberry four categories of six varieties DNJ content was measured, and the results show: DNJ content Monsanto> ghost Sang> long fruit mulberry> Rousan> bilberry> white mulberry> Wah Sang, Monsanto highest content, Wah Sang minimum: Monsanto, ghost Sang, long fruit mulberry three wild mulberry DNJ content in a very significant high in other species; cultivars Wada white mulberry, white mulberry DNJ content of up to 0.11%, the lowest content of weeping mulberry 0.021%; the content of Lusang three varieties of cultivated species were higher for 0.075-0.105%; different local cultivars DNJ content differences. DNJ content in mulberry leaf the slurry juice in extremely significantly higher than mulberry leaves, whole leaf, leaves, vein, DNJ mainly present in the the mulberry leaf slurry juice in. The studies show that the wild mulberry is a good use of the source of the material of the active substance. 4. Using hydrochloric acid extraction, cation exchange purification method respectively DNJ products was obtained from the silkworm powder and mulberry leaf powder 1.4789% and 0.4899%, 62.9269% and 85.7877% purity. The results show that the hydrochloric acid extraction, cation exchange the purification extraction mulberry silkworm DNJ is a viable method. DNJ purity of the product is higher than 60%, is the ideal method. From the tobacco budworm ovarian cultured cells sf9 sf21 and silkworm ovaries cultured cells BmN subjects on three different concentrations DNJ cultured insect cells add processing to cultivate a certain time, MTT assay cell proliferation inhibition rate . Three cell proliferation for 36 hours showed only slight inhibition or promotion of the role, but did not show a certain regularity, indicating that DNJ significantly affect the formation of cell proliferation. Treatment for 72 hours, DNJ role in promoting, dose-response, high to promote the proliferation of high concentration to promote the role of reducing low concentrations; proliferation of sF21 performance inhibitory rate in a dose-effect BmN cell performance, high concentration inhibition rate low concentrations suppressed low, showing the DNJ right sf21 toxic effects; the DNJ on Sf9 cell proliferation promoting effect of different concentrations are higher proliferation promoting effect, but no regularity.

Related Dissertations

CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Traditional Chinese medicine chemical
© 2012 www.DissertationTopic.Net Mobile