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Banlangen is the dried root of Isatis cruciferous plants in China and widely cultivated , is a country that has used Qingrejiedu drug has anti-viral , anti-endotoxin , antipyretic analgesic , anti-inflammatory , blood stasis , anti- cancer effect , the above effect is mainly related to its water-soluble ingredients contained in amino acids , polysaccharides and fat-soluble ingredients indirubin , indigo , and other active ingredients reported in the literature . Banlangen preparations extraction process of water extraction and alcohol precipitation method , the process can obviously cause the loss of fat-soluble active ingredients , this this topic Banlangen extraction process systems research , and of Banlangen preparation of quality standards research . This topic based content indicators Banlangen two active ingredients ( arginine and indirubin ) , the preferred alcohol solution extraction process by orthogonal experimental screening conditions affect the process to determine the 70% ethanol solution adding volume to 12 times the amount of extracted three times , one hour at a time for optimum extraction process . Banlangen granules and prepared according to the USP method . This topic using high performance liquid chromatography Banlangen preparation quality standards . In methanol : water ( 80:20 ) , the join octanesulfonate ( the C 8 < / sub > H 17 < / sub> the NaO 3 < / sub> S ) so that the concentration of 0.005mol / L, adding potassium dihydrogen phosphate ( KH 2 < / sub > PO 4 < / sub >) to a concentration of 0.02mol / L , adjusted to pH2.6 with phosphoric acid as the mobile phase . detection wavelength 206nm liquid phase conditions , arginine content was measured ; tone neutral with acetic acid in methanol : water ( 80:20 ) ( 0.1% diethylamine ) as the mobile phase , detection wavelength 289nm the under liquid phase conditions , indirubin contents were measured according to a measurement result of the triplicate samples draw the present granules arginine content to 9.79mg / g , the indirubin content is 4.12μg / g . The TLC of research and preparation of Banlangen respectively as the control to indirubin and arginine reference substance , n-hexane - dichloromethane - acetone ( 5:4:1 ) and n-butanol - acetic acid - water ( 19:5:5 ) as developing agent , the results showed that the establishment of the TLC method is mature , stable , TLC method as indirubin and arginine . Formulations , the subject the amplification test pilot . Laid the foundation for further large-scale production .
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