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The Application of Flow Injection Chemiluminescence and Its Combination of PLS in Pharmaceutical Analysis

Author: LiShiJun
Tutor: QuLingBo；LiJianJun
School: Zhengzhou University
Course: Drug analysis
Keywords: Chemiluminescence Gold Nanoparticles Pharmacokinetics Partial Least Squares Simultaneous determination of
CLC: O657.3
Type: Master's thesis
Year: 2011
Downloads: 34
Quote: 0
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Abstract


Flow injection chemiluminescence as a sensitivity analysis speed, low-cost analysis, wide linear range, analytical methods, the rapid development of a wide range of applications. Currently, chemiluminescence analysis has become a very active area of ??research, for drug analysis, monitoring of environmental and life sciences, clinical medicine, mineral analysis and immunoassay, chemiluminescence used in the field of drug analysis not only of theoretical significance clinical certification and use of the drug has important practical and guidance. In recent years, chemometric methods and flow injection chemiluminescence method combined not only retains the advantages of high sensitivity chemiluminescence, the equipment is simple, fast, mathematical processing of the measured data, without complex separation process at the same time was out all coexistence content of the component. From traditional chemiluminescent selectivity problem is solved to some extent, to broaden the scope of application of chemiluminescence, chemiluminescence detection has opened up a new research direction. The papers include the following two parts. The first part is a literature review, elaborate chemiluminescence flow injection chemiluminescence and chemometric methods in recent years combined with the progress in analytical chemistry. The second part of the research report, the improved sensitivity of the light-emitting system established first in-depth study, the determination of the two drugs flow injection chemiluminescence analysis. Secondly, the chemiluminescence detection applied to a complex system of pre-treatment technology experimental study. Chemometrics multivariate calibration methods applied to the analysis of chemiluminescence, without separation and pre-treatment to achieve the simultaneous determination of multicomponent complex system. The specific contents are as follows: a flow injection chemiluminescence analysis. Luminol - potassium periodate - gold nanoparticles chemiluminescence detection salbutamol sulphate salbutamol sulfate under alkaline conditions chemiluminescence system of luminol-KIO4 sensitizing The role of gold nanoparticles can be further enhanced chemiluminescence system luminous intensity. Based on a flow injection chemiluminescence method for the determination of salbutamol sulfate. The linear range of 6.0 × 10-7 ~ 1.0 × 10-5g/ml, a detection limit of 9.1 × 10-8g/ml. For Determination of salbutamol sulfate tablets albuterol. 2. Luminol - potassium periodate chemiluminescence method for the determination of to apomorphine hydrochloride under alkaline conditions, has a strong sensitizing effect of apomorphine hydrochloride on the chemiluminescence of the luminol-KIO4 system, under which the establishment of a flow injection chemiluminescence Determination of a new method of apomorphine hydrochloride. The method detection limit is 4.4 × 10-3mg / L, has been used for the Determination of apomorphine in the urine and blood samples, urine samples spiked recoveries were 97.32% -104.69%, blood samples spiked The recovery was 106.30% -110.57%. Flow injection - chemiluminescence method for the determination of puerarin and its pharmacokinetic study the catalyzed hemoglobin (Hb), puerarin significantly inhibited Luminol-H2O2 chemiluminescence system, and the inhibition rate Y puerarin concentration a linear relationship between the number lgc within a certain range of concentrations. According to this phenomenon, the paper established a new method for a simple and sensitive flow injection - chemiluminescence determination of puerarin, the linear range of this method for the 8 × 10-8g/mL 1 10-5g/mL The detection limit of the method for determination of 5 × 10-8g/mL (30), of the 1 × 10-6g/ml puerarin standard solution 11 times in a row parallel, the relative standard deviation of 1.4%. This method has been successfully used for the the puerarin preparation of the three clinical determination. In addition, the combination of solid phase extraction techniques, the method can also be used to puerarin determination and of the urine of rats in vivo pharmacokinetic studies. Second, chemometrics multivariate calibration methods chemiluminescence analysis. Combination of PLS ??flow injection chemiluminescence simultaneous determination of metronidazole and ciprofloxacin in this article, a flow injection the chemiluminescence (FICL) combined partial least squares (PLS) analysis method for the simultaneous determination of metronidazole and ciprofloxacin. The method is based on the difference of both reaction rate kinetics, they luminol - potassium periodate system at different times in different light emission intensity, and then using the partial least squares method for calculating correction. Metronidazole and ciprofloxacin in the optimal conditions for Linear range of 1 × 10-8 × 10-5g/ml and 6 × 10-6-8 × 10-5g/ml, the lowest detection limits were 4.3 × 10-7g/ml9.2 × 10-7g/ml. The method has been successfully used for the simultaneous determination of two samples of tablets. 2. Flow injection chemiluminescence method combined with PLS and determination of the compound rutin film vitamin C and rutin content of this paper, the flow injection chemiluminescence analysis of the simultaneous determination of vitamin C (AA) and rutin (Rutin) method: AA rutin can quantitatively restore Fe (Ⅲ) to generate Fe (II), and the difference between the reaction rate of the two Fe (III) of, the use of potassium ferricyanide - luminol chemiluminescence determination of reduction generated Fe (Ⅱ) in order to determine the content of AA and rutin. Established using partial least squares (PLS) calibration model and predict the chemiluminescence intensity by measuring the different reaction time. Optimized experimental conditions, the the AA and rutin linear range were: 0.1 ~ 20mg / L and 0.02 ~ 0.6mg / L; detection limits were: 0.045mg / L,, and 8.90ug / L. 1.0mg / L the AA and 0.1mg / l RUTIN, the solution 10 times in a row parallel determination of the relative standard deviation (RSD) of 2.80% and 1.42%, respectively. The method is simple, accurate, sensitive, high selectivity, and simple flow path and equipment, practical for these two components in the simultaneous determination of drugs and urine with satisfactory results.

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