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Application of Chemiluminescence in Pharmaceutical and Biological Analysis

Author: LiZuo
Tutor: QiuJianDing
School: Nanchang University
Course: Analytical Chemistry
Keywords: Chemiluminescence Potassium dichromate Metoclopramide Fe3O4 Au NPs @ C Luminol Graphene Chemiluminescence sensor
CLC: O657.3
Type: Master's thesis
Year: 2011
Downloads: 85
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Abstract


Chemiluminescence analysis equipment is simple, easy to automate and other significant advantages, successfully applied in the field of chemical and biological analysis, and other technologies associated with further promote the rapid development of the chemiluminescence technology and expand its application areas. Firstly, the chemiluminescence technology used in traditional medicines analysis for drugs only soluble in acidic media to establish a new method for determining alkaline conditions. Chemiluminescence technology and nanotechnology on the basis of the above work, a combination of ultra-sensitive immunoassay method to construct several new chemiluminescence sensor. The specific content of the paper includes the following sections: 1 chapter chemiluminescence analysis and its application in the analysis of drugs, the status quo as well as the chemiluminescence analysis nanotechnology MS reviewed. Chemiluminescence analysis of the development, concepts, principles and basic device first introduced; details chemiluminescence analysis in pharmaceutical analysis; nanomaterials applications in the field of chemiluminescence analysis. On the basis of the above, the main contents of the papers and conduct. 2, metoclopramide can only be dissolved in the acid medium, so there are certain difficulties in its quantitative detection of chemiluminescence flow injection under alkaline conditions. In acidic medium and high temperature conditions K2Cr207 oxidant metoclopramide (metoclopramide MCPM), while K2Cr207 be reduced to Cr (III) and Cr (Ⅲ) enhancement of the luminescence of the luminol-H202 system with flow injection technique, measured under alkaline conditions MCPM chemiluminescence method. The results showed that the linear range of determination of the method of MCPM 1.0 × 10-8-1.5 × 10-5g/mL, the detection limit of 8.0 × 10-9g/mL, 8.0 × 10-6g/mL MCPM continuous eight times and the relative standard deviation of 2.9%. The sensitivity of the method is high, easy to operate, and can be successfully applied to the tablets MCPM Determination, chemiluminescence detection under alkaline conditions, the MCPM and other drugs is only soluble in acidic media has laid a good foundation. 3, chapter Fe304 nanomaterials fixed anti, Au NPs @ C nano-composite material is fixed secondary antibody and horseradish peroxidase, and using the sandwich immunoassay mode, as the reactor and separator with a double helical tube and coil, to achieve the ultra-sensitive and rapid quantitative detection of human immunoglobulin IgG. The excellent biocompatibility of Au NPs Au NPs @ C nanocomposites laid the foundation to effectively maintain the activity of the protein;, Au NPs @ C nanocomposites large surface area to the high density of the secondary antibody and enzyme load good platform, greatly enhanced chemiluminescence signals, to improve the sensitivity of detection of the target molecule. In addition, the magnetic properties using Fe304 nanoparticles achieve automation of the analytical process, to helical tube reactor effectively promote rapid immune response and the coil separator using magnetic nanoparticles sandwich immunoassay conjugates not combined with complete separation of the two anti-complexes. The analysis of the human immunoglobulin IgG immune detection can be completed within 10 min to detect the linear range of 1.0-1000 ng / mL, the detection limit of 0.74 ng / mL. The analysis method is rapid, practical, and can be widely used for the detection of other proteins and DNA. Proposed A process for preparing a the graphene / 鲁米诺纳米 composite new method, chitosan This composite is fixed in a glass tube, constructed a novel chemical luminescence sensor. This article examines the room temperature mixing, high temperature mixing at room temperature plus EDC / NHS mixture of three different the graphene / 鲁米诺纳米 complex preparation methods of sensor performance results show that, at room temperature through EDC / NHS luminol nanocomposite prepared by the method of crosslinking and the graphene luminol molecules immobilized largest, the strongest response to H202. The effects of chitosan and sol - gel on the the graphene / 鲁米诺纳米 complexes immobilized on the sensor performance results show that chitosan better able to keep chemiluminescence activity of the composite graphene / Luminol / chitosan film sensor linear range of detection of H202 wide, a good linear relationship in the range of 5.0 × 10-6-2.0 × 104 mol / L, the detection limit of 2.7 × 10-6mol / L and 17 consecutive injection of 5.0 x 10-5 mol / L H2O2 standard deviation of 2.6%. The preparation for biochemical luminescence sensor opens up new ways to have a good prospect.

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CLC: > Mathematical sciences and chemical > Chemistry > Analytical Chemistry > Instrument analysis ( physics and physical chemistry ) > Photochemical analysis ( spectral analysis method)
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