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Wild soybean (Glycine soja) is the ancestor of cultivated soybean (Glycine max), formed abundant type of variation in the long-term natural selection, natural soybean gene pool, with a high protein content, strong resistance, and the propagation coefficient other fine features. Our country is the world save wild soybean resources than any other country, and the collection and preservation of the national gene pool of wild soybean resources of more than 6500, more than 90% of the accounting world wild soybean collection materials. Study the genetic diversity of wild soybean not only provide a valuable foundation material for soybean breeding, expand the soybean genetic basis of practical significance, at the same time also of great significance for theoretical issues such as soybean origin and evolution. This test collected wild soybean resources of the coastal beach in northern China (mainly referring to Tianjin and Shandong coastal beach) on the genetic diversity of wild soybean resources collected from the DNA molecule level. The main results are as follows:. Collect wild soybean resources of Tianjin and Shandong coastal beach, collected a total of 242 wild soybean monoclonal, which 72 copies Tianjin, Shandong 172. 2 Select the polymorphism 20 SSR primer pairs diversity analysis of 242 wild soybean monoclonal, 157 alleles were detected, the average variance of each SSR alleles was 7.85, luffing 4 to 16; Simpson index distribution range of 0.3630 to 0.8302, and the average value of .6507; Shannon-Weaver index distribution range of 0.7504 to 2.2176, and an average of 1.3990. 3 in 18 collection sites, each collection to the material within the between Simpson genetic diversity index ranged from 0.1167 to 0.5420, Tianjin Daegu Town Wang Hu Village (collection site); Shannon-weaver genetic diversity index ranged from 0.1724 to 0.9921, Tianjin Liang Wang Township Xiaotun Village (collection site 3). Of 4 geographically 38.5780 ° to 39.1443 ° N 117.0044 ° to 117.6173 ° E district collected wild soybean materials exhibit the highest diversity, Simpson index of 0.6846. 5. Tianjin and Shandong coastal beach wild soybean germplasm Diversity contrast analysis showed that the the Tianjin material amplified 133 alleles, the Simpson index average to .7010, Shandong Materials 120 alleles were amplified Simpson index average is 0.3976, that the genetic diversity in Tianjin significantly higher than Shandong material. 242 wild soybean material clustering analysis showed that the genetic distance of 1.60 basis were divided into four categories, the genetic distance between the various materials ranging from 0 to 1.83, the average genetic distance of 0.216, Tianjin and Shandong material preferably separate areas, and occurs at the molecular level, some genetic differentiation.
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