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Chinese medicine is a treasure house of Chinese medicine, to strengthen the study of Chinese traditional medicine, especially the study of traditional Chinese medicine immune activity has become an important research topic. Recent findings of our group, toad venom and Tibet Picrorhiza Chinese medicine has a role to improve the level of immune, immune activity of representative precursor compounds obtained with this thesis, two traditional Chinese medicine are made more in-depth study of the chemical composition for looking the effective immune Banzhilian active ingredients to provide the scientific and experimental technical basis. The papers of Chinese medicine Chansu and Tibet Picrorhiza chemical composition through a variety of separation methods, 18 compounds were isolated from both traditional Chinese medicine, including four new compounds. The compounds are as follows: 11 compounds isolated from the Chinese medicine Chansu (Venenum Bufonis) separated by silica gel column chromatography, HPLC and recrystallization. In use 1 H-NMR 13 C-NMR, HMQC and HMBC such as modern spectroscopic methods, combined with the physical and chemical properties of the structures of these compounds were identified. Amine compounds include of 10 toads esters and a toad poison color, structure: Chinese toad poison it spirit (cinobufotalin, 1), bufalin (bufalin, 2), the Japanese toad poison spiritual (gamabufotalin 3), Chinese toad venom poisoning base (cinobufagin, 4), fat toad poison ligand (resibufogenin, 5), the the sand the toad poison fine (arenobufagin, 6), the South American toad poison Jing (marinobufagin, 7), Yuanhua toad poison fine ( the telocinobufagin, 8), sneeze blades of grass ligand (hellebrigenin, 9), the deacetylase Chinese toad poison it spiritual (desacetylcinobufotalin, 10) and the dehydrogenation toad poison serotonin (dehydrobufotenine, 11), and for the first time on the compound 11 1 H-NMR and 13 C-NMR signals were completely assigned. 7 compounds from traditional Chinese medicine in Tibet Picrorhiza (Picrorhiza scrophulariiflora) isolated and purified by macroporous resin column chromatography on silica gel column chromatography, gel column chromatography, HPLC and recrystallization separation method. The use of MS, 1 H-NMR 13 C-NMR, HMQC, HMBC and TOCOSY modern spectroscopic methods, combined with the physical and chemical properties analysis of the structure of these compounds were analyzed identification. Eight compounds were identified as: D-mannitol (D-mannitol, 1), possession of Coptis caffeic acid glycoside C (scrocaffesideC, 2), Tibetan coptis glycoside I (scroside I, 3), the possession coptis caffeic acid glycoside B (scrocaffeside B, 4), possession the yellow comic the allyl ethers terpene glycosides I (picrogentioside I, 5) the possession of Coptis glycosides D (scroside D, 6) and possession of Coptis glycosides E (the scroside E, 7). Which compounds 2-5 are new compounds.
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