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Study on the Effects of Puerarin on Mammary Glands Growth and the Uteruses Growth in Ovariectomized Mice

Author: WuYingGa
Tutor: BaYinJiRiGaLa
School: Inner Mongolia Agricultural University
Course: Clinical Veterinary Medicine
Keywords: Puerarin Ovariectomized Breast Uterus Estradiol Progesterone Growth Hormone Prolactin
CLC: R285.5
Type: Master's thesis
Year: 2008
Downloads: 78
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Abstract

Experiment the establishment of a normal control group, negative control group (ovariectomized group), the experimental group (ovariectomized puerarin group), positive control group Ⅰ (ovariectomized DES group) and a positive control group Ⅱ (to in ovariectomized DES P group), puerarin on the 21-day-old ovariectomized mice mammary gland development, development of the uterus, the impact of weight gain and serum hormone content changes. The experimental results show that: 1. Negative control group (ovariectomized group) compared with the normal control group has the following changes in each index: mouse slow growth, the total weight increase was reduced (P lt; 0.01); mouse ovarian hormone target organs - breast and uterine development is limited, ductal and acinar reduce the number of (P lt; 0.01), the breast / breast domain values ??reduce (P lt; 0.01), uterine coefficient reduce (P lt; 0.01), uterine reduce the number of the gland (P lt; 0.05); serum estradiol (E2) levels reduce (P lt; 0.05) and progesterone (P) content to reduce (P lt; 0.05), growth hormone (GH) levels increased ( P lt; 0.05), prolactin (PRL) showed no significant change (P gt; 0.05). Compare each index in the positive control group Ⅰ (ovariectomized DES group) and a positive control group Ⅱ (ovariectomized the DES P group) and the negative control group has the following changes: the growth of the two groups could promote the mice, the first week's weight increase were significantly higher (P lt; 0.05); continued growth and development of the mammary gland and uterus of mice, ductal and acinar number were increased (P lt; 0.05), breast parenchyma / breast domain values ??were increased, the difference respectively (P lt; 0.05 and P lt; 0.01), uterine coefficients were higher (P lt; 0.05), the number of uterine glands were increased (P lt; 0.05); serum estradiol (E2) showed no significant change (P gt; 0.05), progesterone (P) levels significantly increased (P lt; 0.05), growth hormone (GH) content were lower (P lt; 0.05), prolactin (PRL) levels did not change significantly ( P gt; 0.05). The experimental group (ovariectomized puerarin group) of the total increase in body weight of mice increased, and a significant difference with the negative control group (P lt; 0.05). Puerarin growth-promoting effect is not obvious, no significant difference (P gt; 0.05) and the negative control group of mice the first week of weight gain; mouse mammary and uterine indicators data are slightly higher, but with the negative control group showed no significant difference (P gt; 0.05); raise serum estradiol (E2) levels to a certain extent, compared with the normal control group no significant difference (P gt; 0.05), but also with the negative control group, no significant differences (P gt; 0.05). Progesterone (P) levels increased (P lt; 0.05), and a significant difference with the negative control group (P lt; 0.05). Growth hormone (GH) decreased, and a significant difference with the negative control group (P lt; 0.05). The content of prolactin (PRL) and negative control group, there was no significant difference (P gt; 0.05). Experiment II established the control group and the experimental group and the control group mothers with distilled water, the experimental group mothers the the puerarin solution gavage, puerarin production in mice milk yield and its offspring weight gain. Experimental results show that: the experimental group compared with the control group, puerarin production in mice prolactin trends, and slightly increase the weight gain of the pups, but there was no significant difference (P gt; 0.05). These results suggest that puerarin and diethylstilbestrol (DES) and diethylstilbestrol (DES), progesterone (P) ovariectomized mice serum hormone levels are basically the same, but the puerarin estrogen target organ - the role of the breast, uterus The obvious; puerarin has some role in animal health, the role of prolactin significant. This experiment no side effects of natural estrogen replacement drugs or animal health drug development, application and provide experimental basis.

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