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Additives and Italian ryegrass silage grass objects Fermentation Quality and Aerobic Stability studies

Author: ZhangLei
Tutor: ShaoTao
School: Nanjing Agricultural College
Course: Grassland
Keywords: Elephant grass Italian ryegrass Additive Fermentation Quality Aerobic stability
CLC: S816.53
Type: PhD thesis
Year: 2010
Downloads: 184
Quote: 0
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Abstract


Elephant grass, Italian ryegrass is cultivated in southern China region is an important forage, high yield, good quality, but there are obvious forage seasonal variations, production of high quality silage can balance the elephant grass and Italian ryegrass in Animal Production annual supply, especially for the South livestock dairy industry to provide more high-quality feed. Experimental study of the major objects of different types of grass and silage additives Italian ryegrass silage fermentation quality and aerobic stability, to clarify their effects on silage in this study based on the combined addition of inhibitors and accelerators and alternative additives on grass silage fermentation, in order to improve the quality of silage fermentation and related additives provide a theoretical basis for the development. An object sorbate grass silage fermentation quality and aerobic stability of the effect of the different dosage of sorbic objects grass silage fermentation process and silage aerobic stability. Five days before fermentation, addition of sorbic acid reduces silage acetic acid and AN / TN, improved silage WSC content of 0.10% over the amount of added acetic acid treatment was significantly (P lt; 0.05) lower than the control. As for silage fermentation, lactic acid fermentation gradually dominated, pH decreased rapidly lactic acid content of each treatment was first increased and then decreased. Sorbic acid in the fermentation process with added increases, the adding process of lactic acid has a reduced tendency decreased acetic acid, and reduced silage in the number of yeasts and molds. Fermentation 14 days, with the increasing of the addition, the process reduces the total content and the VFAs AN / TN, improve the LA / AA and WSC content. The above results show that the addition of sorbic acid can effectively inhibit the early fermentation aerobic microbial activity, improve the silage fermentation quality. In silage exposed to air 0-8 days, and 0.05% were treated pH gradually increased, the value of the first eight days of the day with other significant difference (P lt; 0.05), and 0.10% more than sorbic acid treatment in aerobic phase of pH change is small, and significantly (P lt; 0.05) lower than the control value of each day. Compared with the control, delaying the addition of sorbic acid content of lactic acid silage aerobic phase rate of decline, and with the increasing of the addition of sorbic acid, lactic acid content in silage and LA / AA gradually increased, adding 0.10 percent above processing significant difference compared with the control (P lt; 0.05). In the aerobic phase sorbate reduces silage of volatile fatty acids and AN / TN, increased WSC content, and more than 0.10% and 0.05% with the control treatment difference was significant (P lt; 0.05). These results show that adding the elephant grass silage than 0.10% sorbic acid, can effectively inhibit the silage exposed to the air, aerobic microbial activity, improve aerobic stability of silage aerobic reduce corruption. 2 Ethanol objects grass silage fermentation quality and aerobic stability of the effect of the different amount of ethanol added objects grass silage fermentation and aerobic stability of silage. The results showed that the initial three days of ethanol treatment decreased the pH of the slow pace compared with the control, three days after the pH value decreased greatly. As for silage silage fermentation lactic acid content showed an increasing trend and reached a maximum of 7 days, and 30 days after the lactic acid content of ethanol were higher than controls. The beginning of the fermentation, the water-soluble carbohydrate content silage decreased rapidly, adding ethanol and water soluble carbohydrates silage lactic acid content were higher than the control, and the adding process improves the utilization efficiency of water soluble carbohydrates. In the silage process, the added processing silage ammonia / nitrogen than the control treatment, adding ethanol inhibits harmful microorganisms on protein breakdown. Ethanol was added silage fermentation process reduces the content of volatile fatty acids and the AN / TN, and with the increase in the amount of ethanol added, volatile fatty acids and AN / TN was gradually decrease. In the aerobic phase pH rapid increase in the control group, while ethanol silage pH increased to a lesser extent, the pH of each day were lower than the control, the treatment of lactic acid, WSC content decreased as the number of days, but the ethanol processing content decreased more slowly, and with the increase in the amount added is raised. Control treatment of Ammonium / TN increases aerobic phase, and significantly (P lt; 0.05) more than 1.5% of the ethanol treatment. Considering the above test results, 1.5% more than the amount of ethanol added can effectively improve the elephant grass silage fermentation quality and aerobic stability. 3 sorbic acid, ethanol and molasses, fermented juice combinations add objects grass silage fermentation quality of this trial studied the addition of sorbic acid, ethanol, molasses, fermented juice and combinations thereof objects grass silage fermentation quality and water-soluble carbohydrate content. Elephant grass silages were added 0.10% sorbic acid, 1.5% ethanol, 5ml kg-1 as the grass juice broth, 1.0% molasses, and combinations thereof (both representing the ratio of fresh weight), the results show that the elephant grass silage adding 1.5% ethanol and 0.10% sorbic acid were lower pH, volatile fatty acids and AN / TN, improved silage lactic acid, LA / AA (P lt; 0.05) and WSC content. Molasses 1.0%, which was significantly (P lt; 0.05) increased the silage acid, water-soluble carbohydrate content, reducing the pH and the AN / TN, improve the LA / AA. Fermented juice and molasses mix adds improved silage lactic acid, WSC content, reducing the AN / TN. 1.5% ethanol and 1.0% of the combined addition of molasses and alcohol, molasses, fermented juice combination of the three added processing, have reduced silage volatile fatty acids were significantly (P lt; 0.05) increased dry matter content and WSC content. Sorbic acid and sorbic acid with molasses, molasses, fermented juice combinations add significantly (P lt; 0.05) lower pH, AN / TN, was significantly (P lt; 0.05) increased lactate, LA / AA and WSC content. General indicators silage, 1.5% ethanol and 1.0% molasses and 0.10% of the combined addition of sorbic acid and 1.0% of the combined addition of molasses in all combinations in the best addition process. 4 tannins objects grass silage fermentation quality and aerobic stability of this study was to investigate the influence of tannins objects grass silage fermentation quality and aerobic stability of silage. The results show that tannic acid reduces the acid content of the silage and LA / AA, with the amount of tannic acid added increases, gradually increasing the pH of the silage, 0.5% treated was significantly (P lt; 0.05) higher and 0.3% in the control treatment. Compared with the control, tannic acid treatment increased silage volatile fatty acids, reducing the AN / TN. Silage pH gradually exposed to the air, elevated pH 6 days in the control group was significantly (P lt; 0.05) greater than 0.4%, the above process, the process of lactic acid content gradually decreased, reached the minimum on day 12, and the control section 12 days was significantly (P lt; 0.05) lower than the tannic acid treatment. In the aerobic stage of volatile fatty acids in each treatment was gradually decreasing trend, and tannic acid treatment was higher than the control. Each treatment AN / TN gradually increased, tannic acid treatment than the control, with the increase of added amount of tannins AN / TN showed a decreasing trend. Tannins inhibit the fermentation process and the aerobic phase protein degradation, 0.4% addition of tannins can effectively improve the aerobic phase of elephant grass silage stability. 5 different silage grass silage fermentation inhibitors target quality of this experiment the effects of different aerobic microbial inhibitors sorbic acid (0.10%), ethanol (2.5%), formic acid (4mlkg-1), tannic acid (0.40%) Object grass silage fermentation quality. The results showed that, compared with the control, the added processing improves silage pH, acid treatment significantly (P lt; 0.05) higher than the control. Addition of sorbic acid treatment, the added processing of lactic acid content was significantly (P lt; 0.05) lower than the control, and sorbic acid treatment significantly (P lt; 0.05) higher than that of formic acid and tannic acid treatment. Tannic acid treatment of acetic acid and total volatile fatty acid content higher than the control, the content of other treatment than the control, but the difference between treatments was not significant (P gt; 0.05). Sorbic acid and ethanol processing LA / AA higher (P gt; 0.05) were added and other processing. Added to each treatment were improved silage residual WSC content, except sorbic acid treatment, the remaining WSC adding process was significantly (P lt; 0.05) higher than the control, formic acid treatment was significantly (P lt; 0.05) higher than Other added processing. Adding inhibitors of this test are different degrees of inhibition of microbial activity of aerobic fermentation process, improved residual silage WSC content, but the lactic acid bacteria have a certain extent, reduced lactic acid. Comprehensive comparison of various indicators of silage, this test additive effect of each order: sorbic acid gt; ethanol gt; acid gt; tannins. 6 lees mixed with Italian ryegrass silage and aerobic stability of this experiment study of distillers grains in different proportions mixed with Italian ryegrass silage fermentation and aerobic stability of silage. Lees mixing ratio was 0,10%, 20%, 30%. With the lees mixing ratio increases, decreasing the pH of silage, distillers 7 days before the pH of each treatment were significantly (P lt; 0.05) lower than the control, lees than 20% after 14 days treatment and control pH difference was significant (P lt; 0.05). Promote lactic acid fermentation lees, lees handled more than 20% lactic acid and LA / AA was significantly (P lt; 0.05) higher than the control, 30 days after the treatment of the lees lactate levels were higher than the control, and 20% with a significant difference from the control ( P lt; 0.05). Lees treatment significantly (P lt; 0.05) reduces silage acetic acid and volatile fatty acid content, and with the increase in the proportion lees, silage acid content showed a decreasing trend in the first 30 days 30% acetic acid was treated (P lt; 0.05) lower than the control. Lees treatment decreased silage AN / TN, and with the lees mixing ratio increases, AN / TN showed a decreasing trend. Early treatment delayed the fermentation lees silage WSC falling faster, improved residual WSC content, the first 30 days stillage processing WSC were higher than the control, and the 30% difference between treatment and control was significantly (P lt; 0.05). Silage exposed to the air lees slowed silage lactic acid, WSC content rate of decline, lees treatment of lactic acid, WSC content than the control, lees than 20% after 8 days treatment and control significant difference (P lt; 0.05). The total aerobic stage silage VFAs increased first and then decreased, LA / AA decreased, lees treated LA / AA higher than the control. In the aerobic stage lees treatment decreased silage AN / TN, 30% of the value of the deal was significantly (P lt; 0.05) lower than the control. Italian ryegrass silage mixed with the lees can improve the content of lactic acid, acetic acid reduction, reducing the loss of protein and WSC content in the aerobic phase delay WSC content of lactic acid and the rate of decline, reducing the aerobic corruption, and more than 20% stillage processing better.

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CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > General Animal Science > Feed > Roughage > Silage
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