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In recent years, a large number of scientific findings, miniature pigs and Comparative Medicine homologous closer relationship is very similar in anatomy, physiology, biochemistry, and disease mechanism. Is often used as experimental animals in the cardiovascular system, digestive system, reproductive system, endocrine system, geriatrics, hematology, nutrition, skin, dental, ophthalmology, radiation biology and immunology research in recent years for xenotransplantation The study also gradually increased. However, in miniature pigs used in animal models of Medicine, transgenic animals, cloning, organ transplantation, embryo transfer process, the need for surgery. No anesthesia, miniature pigs can not tolerate to live strong surgical pain stimulus, it is impossible to complete the precise surgical procedures and research process, existing anesthetic pigs can not produce good anesthesia in urgent need of a way to anesthetic pigs anesthetic effect. The Northeast Agricultural Department of Surgery at the University this purpose in accordance with the theory of balanced anesthesia and physiological characteristics of miniature pigs developed a ketamine as the main component and composite midazolam, jingsongling and strong Fengtongning composite anesthetic, into clinical required before widespread use of the various clinical anesthetic effect and parameters were measured in order to provide reference for the clinical use of drugs. In this experiment, Bama miniature pigs as experimental animals, the use of intensive care instrument miniature pigs during anesthesia body temperature, heart rate, respiratory rate, oxygen saturation, blood pressure and ECG monitoring, EEG EEG monitoring, observation its anesthetic effect; actual surgery and create a feeling of Lung Peng composite anesthetic compared to evaluate the effect of the anesthetic ketamine combined anesthetic; using high performance liquid chromatography (HPLC) determination of the plasma concentration of ketamine in small pigs - time curve , medication Pharmacokinetic software 3p87 analysis, study its pharmacokinetic characteristics. The experimental results show that: the induction of anesthesia ketamine combined anesthetic miniature pigs to 2.9 ± 0.8min, narcotic period of 88.6 ± 10.6min, the awakening of 37.1 ± 5.9min, small pig's body temperature range between 37.22 ℃ to 38.42 ℃, heart rate between 64.25 beats / min to 94.37 times / min, respiratory rate between 30.25 beats / min to 46.50 times / min, oxygen saturation between 92.62 to 97.62%, the mean arterial pressure in 71.37mmHg to 100.25mmHg between. Contrast and create a feeling of HSING composite anesthetic surgical verification experiment, create a feeling of composite anesthetic, anesthetic ketamine combined effect is better than to be able to provide good analgesic, sedative and muscle relaxant effects surgery. This experiment, a small pig plasma ketamine concentrations were determined using the HPLC method. Sample pre-treatment method is simple, practical, high recovery rate, the method is reliable. This method is suitable for clinical anesthesia blood concentration monitoring and pharmacokinetic studies. This method to the determination of the concentration of ketamine in 0.2μg/ml ~ 3.2μg/ml linear relationship good standard curve correlation coefficients (R2) greater than 0.99. Miniature pigs intramuscular ketamine combined anesthetic ketamine pharmacokinetic characteristics consistent with a kinetic one-compartment model, which ketamine pharmacokinetic parameters were T1 / 2 = 9.003 ± 1.032 min, Cmax = 2.871 ± 0.853 μg / ml, T (peak) = 4.684 ± 0.042 min, V / F = 0.243 ± 0.012 (mg / kg) / (μg / mL), AUC = 53.477 ± 5.368 (μg / ml) × min, T1 / 2 ( Ka) = 1.515 ± 0.015min. In summary, ketamine combined anesthetic miniature pigs has good sedation, analgesia and muscle relaxation, physiological functions, a wide range of security, easy to use, and can meet the clinical anesthesia need, can be used in clinical practice. As can be seen from the pharmacokinetic parameters, the small pigs after intramuscular injection of ketamine composite anesthetic ketamine rapidly absorbed in the small pigs and subsequently rapidly distributed to the organization, but the tissue concentration is less than the plasma concentration in the body to eliminate quickly.
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