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The Expression of IL18Rα in Inferior Mesenteric Ganglion and Lumbar Dorsal Root Ganglions of Female Goats

Author: WangHongMei
Tutor: XuYongPing
School: Northwest University of Science and Technology
Course: Basic Veterinary Science
Keywords: IL-18Rα Mesenteric ganglion Lumbar dorsal root ganglion Female goats
CLC: S827
Type: Master's thesis
Year: 2011
Downloads: 6
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Abstract


Immune body to identify and exclude an important function of the antigen foreign body, and is regulated by the nervous, endocrine and other factors as the local immune status of the female reproductive system gave birth to a new life. To explore whether TH1-type cytokines by both sympathetic and sensory nerve pathways regulating the physiological state of the female reproductive system, these cytokines its receptor binding can play a biological activity. Therefore, this experimental molecular biology and proteomics to explore the expression and localization of IL18Rα female goats mesenteric ganglion and lumbar dorsal root ganglion confirmed IL18 role in visceral feeling pass through direct or indirect means into sympathetic efferent neurons, the activities of which affect the reproductive physiology of the uterus to provide morphological evidence for the study of the female reproductive system neuroimmunomodulation. The main experimental results obtained in this study are as follows: 1 This research makes use of molecular biology methods for seed in order to the sequence of IL18RαcDNA of cattle, pigs, people, and for the first time from a goat lumbar dorsal root ganglia and mesenteric ganglion cloned 447 bp the goat IL18Rα gene partial cDNA sequence, wherein 5 to 445 nucleotides encoding 148 amino acids. By bioinformatics software goat IL18Rα part of the gene sequence and amino acid sequence homology comparison with other species, found in the amino acid homology comparison, the amino acid sequence of the segment with species such as cattle, swine, and human have a high degree of similarity respectively 100%, 90%, 88%; gene sequence homology alignment results show that with the bovine, porcine, human IL18Rα homology reaches 91%, respectively, 88%, 87 %. 3. To the clones obtained IL18Rα portion gene as template for the preparation of in vitro reverse transcription-specific oligonucleotide probes. In situ hybridization to detect the the female goat mesenteric ganglion and lumbar dorsal root ganglion a IL18RαmRNA expression and localization, the results show the superior mesenteric ganglion IL18RαmRNA mainly localized in the cytoplasm of neurons, satellite cells and neurons lumbar dorsal root ganglion tissue cells; IL18RαmRNA in neurons nuclear membrane, cytoplasm of neurons, satellite cells, glial cells and myelinated nerve fibers were expressed. 4 and the use of Western Blot and immunohistochemistry SP method validation from the protein level IL18Rα female goats mesenteric ganglion and lumbar dorsal root ganglion and positioning. The results showed that the expression of its mRNA positioning basically consistent, mesenteric ganglion IL18Rα mainly present in the cytoplasm of neurons, satellite cells, there are also some positive particles between glial cells and neurons in the lumbar dorsal root ganglion IL18Rα in neurons nuclear membrane, cytoplasm of neurons, satellite cells, glial cells and myelinated nerve fibers showed positive expression was negative in the nucleolus.

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CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Livestock > Goat
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