|
In this paper 549 Tou West Nong Sa can milk the goat and Guanzhong milk goat for the test material, the use of PCR-SSCP, of PCR-RFLP, of DNA sequencing and biological information science technology, research the Xinong Saanen and Guanzhong milk mountain goat milk iron protein gene (lactoferrin gene, LF) for all 17 exon gene locus genetic variation, to explore two dairy goat breed genetic polymorphisms and body sizes of dairy goats traits (body height, body length, chest), milk yield and milk ingredients (milk protein, milk fat, lactose, total milk solids non-fat solids, milk pH value), in order to provide a scientific basis for economic traits of molecular genetic markers for molecular breeding of dairy goats. Obtain the following important results of this study: 1 in 17 sites in the dairy goat lactoferrin gene (P1-P17), P2, P4, P6, P10 locus only found five SNP loci. Detected in the P2 locus in two dairy goat breeds, AA, GG, AG three genotypes; P4 loci detected by CT and TT genotypes; 2 chain SNP loci detected in the P6 locus two loci, respectively, the performance of the TT, TG genotype AA, AG genotype; P10 loci detected TC and CC genotypes. Lactoferrin gene P2 loci detected GG, GA, AA genotype, Xinong Saanen and Guanzhong dairy goat G / A allele frequencies were 0.9552/0.0448, 0.9448/0.0552; West farmers Saanen and Guanzhong milk Goats are low in the P2 locus degree of polymorphism (PIC lt; 0.25), and in the Hardy-Weinberg disequilibrium; the P2 locus of genotypes in West agricultural Saanen and Guanzhong dairy goat breed distribution difference was not significant (P gt; 0.05). Lactoferrin gene P4 loci detected TT and TC genotypes, Xinong Saanen and Guanzhong dairy goat T / C allele frequency were 0.8806/0.1194, 0.9075/0.0925; the Saanen and off in the Xinong dairy goats P4 locus are low polymorphism (PIC lt; 0.25), Saanen dairy goats in Hardy-Weinberg disequilibrium Guanzhong dairy goats in Hardy-Weinberg equilibrium; P4 locus the genotypes Xinong Sa and the distribution of differences between the Guanzhong dairy goat breeds was not significant (P gt; 0.05). Both chains genotype in lactoferrin gene P6 loci detected: TT, TG, AA, AG, Xinong Saanen and Guanzhong dairy goat T (A) / G (G) allele frequencies were 0.9164/0.0836, 0.9216/0.0784; Xinong Saanen and Guanzhong dairy goat in the P6 locus the P6 locus genotypes are low polymorphism (PIC lt; 0.25), and in the Hardy-Weinberg equilibrium; in West agricultural Saanen, and the distribution of differences between the Guanzhong dairy goat breeds was not significant (P gt; 0.05). Detected in the lactoferrin gene P10 locus of CC, TC genotype, Xinong Saanen, and off dairy goat T / C allele frequencies were 0.8657/0.1343 0.8897/0.1103; Xinong Saanen and Guanzhong dairy goat P10 loci are low polymorphism (PIC lt; 0.25), and in the Hardy-Weinberg disequilibrium; the the P10 locus of genotypes in West agricultural Saanen and Guanzhong dairy goat breed distribution difference was not significant (P gt ; 0.05). 2 found lactoferrin gene P2 and P4 locus genotype was significantly associated with economic traits of two dairy goat breeds. SPSS (16.0) software, the use of the method of least squares fitting a linear model to analyze five mutation sites found P6, P10 locus genotype and no significant body size and milk production of the two dairy goat breeds related (P gt; 0.05). Fat, protein, dry matter, non-fat solids, lactose content and milk pH with milk ingredients and no significant impact (P gt; 0.05). P2 locus GG, GA and AA genotypes found two dairy goat breeds AA genotype GG genotype (P lt; 0.05) was significantly higher than its body. Two dairy goat breed milk ingredients, fat, protein, dry matter, non-fat solids, lactose content and milk pH of six indicators, milk protein content and pH of the AA genotype AG and GG genotype were higher than individuals (P lt; 0.05). Different genotypes of the other four indicators had no significant effect (P gt; 0.05). P4 locus TT and TC genotypes, two dairy goat breeds TC genotype milk fat content was significantly higher than the TT genotype (P lt; 0.001), total dry matter content and pH value of the TC genotype significantly higher than the TT genotype (P lt; 0.05). Therefore, the preliminary view that the lactoferrin gene can be an important candidate genes as economic traits of dairy goat breeding P2 and P4 locus.
|