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Superovulation of Lambs and Its Fertilization in Vitro

Author: WuHaiFeng
Tutor: ZhangYong
School: Gansu Agricultural University
Course: Developmental Biology
Keywords: Oocytes Lamb Superovulation In vitro maturation In vitro fertilization
CLC: S826
Type: Master's thesis
Year: 2011
Downloads: 26
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Abstract


Young lambs superovulation technology is the invention before sexual maturation in young female lambs (1-2 months old) reproductive hormone sensitive ovarian follicular development rarely occurs atresia special physiological characteristics of a high-efficiency production of sheep oocytes The cells of the new technology. Young lamb super early breeding technology is young lamb superovulation living donor ovum, in vitro fertilization, embryo transfer technology embryo biotechnology system. Trial lamb sheep were studied juvenile lamb sheep superovulation, in vitro oocyte maturation and fertilization effect and repeated hormone treatment of young lamb Superovulation young lambs compared superovulation mature, the collected oocytes and adult sheep ovarian oocytes obtained from slaughterhouse vitro in vitro fertilization and early embryonic development ability, and semen pH young lamb oocytes in vitro fertilization and early embryonic development ability test to perfect \The results are as follows: 1. Superovulation with FSH PMSG and FSH LH two different programs on the 8-week-old Alpine Merino 4 injection of saline as a control group. Superovulation a total number of 583 developing follicles recovered oocytes 255. Oocytes sperm can be cultured in vitro for 24 h with the upstream method for in vitro fertilization and cleavage rate in each group, respectively, 11.4% and 22.7% after 48 hours. The mature oocytes FSH PMSG treated by in vitro fertilization after FSH LH cleavage rate of the treatment group was significantly higher than rates of cleavage of the egg. Month interval, to repeated superovulation eight lambs in the experimental group with the same method, the results indicate that repeated superovulation number of developing follicles and the available number of oocytes obtained 20 and 15, respectively, significantly lower than the first The times the superovulation average number of developing follicles, average available number of eggs each were 54 and 36. 3. Lamb oocyte maturation rate of 41.9%, significantly lower than that obtained from the slaughterhouse adult sheep ovarian oocytes in vitro maturation rate of 75.0%. Lamb oocytes, fertilization and cleavage rate and morula rate of 44.4% and 37.5%, respectively, were significantly lower than the adult sheep ovarian oocytes cleavage rate of 75.8% and 70.0% morula. 4 lamb oocytes in vitro fertilization and embryo development results showed that sperm were incubated the time 24h group fertilized egg split was 60.4%, significantly higher than 25.0% of the 16h group and 33.33% of the 27h. 5. Semen pH split rate of oocytes fertilized egg has a significant impact on the test results showed that the pH of 7.3 to 7.5 by the semen oocyte cleavage rate of 56.6% was significantly higher than at pH 7.6 to 7.8 by semen cleavage rate of 32.5%, (P lt; 0.01).

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CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Livestock > Sheep
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