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Research of the papers in the Summary of indole butyric acid, the direction of development of the domestic and international pesticide formulations, the development of nanotechnology, on the basis of nano-suspension technology Description and cutting propagation, to improve the efficiency of the plant cutting propagation and seedling breeding , to improve the efficacy of drugs and effective utilization degree, using the ultrasonic emulsification prepared indol butyric acid nanosuspension process applied Euonymus Greenwood cuttings, compared with the indol butyric original agent, the formulation on Euonymus japonicus Greenwood Cutting effect is significantly strengthened. Research work and the results are as follows: 1 Preparation of indole butyric acid nanosuspension: by ultrasonic emulsification method, indole butyric acid concentration of a suspension for the 500 mg / L Tween 80, an amount of 1%, the sample injection speed 0.1 mL / s, phacoemulsification time of 60 min. 2, indole butyric acid nanosuspension Characterization: a scanning electron microscope, the average particle size of up to 250 ~ 300nm, and observed under a transmission electron microscope, the maximum particle size of 330nm, infrared spectroscopy, The basic structure and composition has not changed, and better stability. 3, indole butyric acid nanosuspension promote the Euonymus Greenwood rooting effect test: In mid-October, the acquisition the maternal base of the plant sprouting, tops, and from the clipping with buds, at 150mg / L indole butyric acid The nanosuspension soaked base 6h cuttings in vermiculite matrix. The results showed that: (1) Compared with the indole butyric acid reducing agent, indole butyric acid nano-suspensions Euonymus japonicus Greenwood Cutting promoting effect more obvious. When the concentration of 150mg / L, the treatment group as compared with the same concentration of the reducing agent, the formation of the callus are ahead of time and adventitious root formation time of 4D, average rooting rate of 14.59%, 23.69% increase in the average number of adventitious roots, the effect is obvious ; when the concentration of 200mg / L, the treatment group as compared with the same concentration of the reducing agent, the longest adventitious root length and the diameter of the root pieces were increased by 29.07% and 18.87%, the effect is significant; when the concentration of 100mg / L, with the same concentration of reducing agent treatment group, the average root diameter increased by 22.5%, the effect is significant. (2) Euonymus Greenwood Cutting process within the cuttings rooting nutrients and enzyme activity over cutting time and showed a certain regularity changes. 150mg / L indole butyric acid nano-suspensions treated, for example, 10 days after cutting, the total content of chlorophyll and soluble protein content and soluble sugar content reached the highest treatment group compared with the same concentration of the reducing agent, respectively. 37.25%, 37.66% and 83.74%, respectively, compared with the water control treatment group, respectively 58.47%, 51.43% and 99.47%, respectively; cuttings after 30 days, the base of the cortex POD activity and basal cortex PPO activity reached the highest, with the same concentration of reducing agent treatment group compared to 19.41% and 23.26%, respectively, compared with the water control treatment group, respectively 38.78% and 30.86%, the effect is significant.
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