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Hydrangea varieties H. macrophylla'Coerulea ' is an important ornamental plants , the paper under aluminum stress physiological and biochemical characteristics of their tissue culture made ??a study found a strong aluminum tolerance . Specifically: 1 medium MS 6-BA1. Omg / L NAAO.2mg / L suitable for shoot induction . Aluminum Stress medium solidification experiments results show that when nutrients, sterilization temperature , sterilization time is consistent, and agar at pH conditions of the same amount , A1 higher the concentration , the worse the agar solidified . Agar concentration and amount of the A1 under the same conditions , pH, high solidification is better. Al concentration and pH in the same conditions , the greater the agar agar better coagulation . (2) With the increasing concentration of aluminum stress and time of growth , the size of tissue culture there is a clear contrast , where the concentration of 0.25-lmmol / L than the control between the plantlets grow bigger and more lush . From 3mmol / L starting plantlets size and coercion with virtually no change , there was little roots grow , leaf bleaching, appear purple , and even withered and died . This measure of total superoxide dismutase (SOD) and peroxidase (POD) activity , soluble protein (Protein) , malondialdehyde (MDA) content , chlorophyll (Chl) content, soluble sugar content (SS) , membrane permeability (IL) and proline (Pro) content of eight physiological indicators and analyzed . The results showed that , SOD activity and soluble protein content , chlorophyll content was negatively correlated with the concentration of aluminum , the rest was a positive correlation among the soluble sugar content , membrane permeability , proline content in these three indicators and concentration showed a significant positive correlation . Comprehensive view of each index , hydrangea H. macrophylla'Coerulea ' of plantlets for aluminum tolerance can be achieved 2mmol / L, low concentrations of aluminum can promote the growth of plantlets Hydrangea . 3 In this paper, PBS solution and two extraction electrode buffer solution to extract the proteins analyzed by SDS-PAGE technique under different concentrations of aluminum stress proteins in tissue culture Hydrangea difference situation , experiments show that the two can extract effectively extract the protein, but the electrode buffer solution of proteins extracted more sharper bands . Stress concentrations of each protein bands did not show significant differences .
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