Dissertation > Excellent graduate degree dissertation topics show

Study on Vitis Somatic Embryo Induction and Chinese Wild Vitis Stilbene Syntase Gene VpSTSgDAN2 Transformation

Author: XiaoYu
Tutor: WangYueJin
School: Northwest University of Science and Technology
Course: Of Horticultural Plant Germplasm Resources
Keywords: Grapes Somatic embryogenesis Agrobacterium-mediated Stilbene synthase Promoter
CLC: S663.1
Type: Master's thesis
Year: 2011
Downloads: 49
Quote: 0
Read: Download Dissertation

Abstract


Stilbene synthase (stilbene synthase, STS) gene and its specific promoter-based research group pre-associated genes in Chinese wild the Huadong grapes Shirakawa -35 -1 cloned disease resistance. In this study placed to add the stilbene synthase gene enhancer promoter, to build four plant expression vector, aimed at using genetic engineering techniques, it is transferred to a sense of the disease in European grape genome, acquired resistance improve the transgenic plants to provide theoretical and technical basis. 'Jiali stuffed', 'Thompson Seedless', 'Flame Seedless', 'long spike Seedless', 'Cabernet Sauvignon' floral organ explant somatic embryogenesis and seedling research based on Agrobacterium-mediated transformation, will carry the plant expression vector of wild grape stilbene synthase gene-specific promoter into the tomatoes and grapes, the main results obtained are as follows: 1. 5 kinds of European grapes varieties 'Carignan', 'Thompson Seedless', 'Flame Seedless', 'the long spike Seedless', 'Cabernet Sauvignon' stamen, pistil and small buds as explants cultured in four different based on the induction of callus, which medium NN69 2.0 mg · L -1 6-BA 1.0 mg · L -1 2,4-D, 60 g · L -1 sucrose 3 g · L -1 phatagel and medium MS 0.55 mg · L -1 2,4-D 0.5 mg · L -1 NOA 1.24 mg · L -1 4-CPPU 30 g · L -1 sucrose 3 g · L -1 phatagel floral organ explants embryogenic callus induction favorable, the induction rate up to 6.2%. Studied five European grape varieties, in addition to the 'Cabernet Sauvignon', and the remaining four species by the floral organs induced callus available in medium X6 60 g · L -1 sucrose, 7 g L -1 the TC-Agar 0.5 g · L -1 AC further induced somatic embryos. And X6 medium relay generation to save for the developmental stages of somatic embryos before the heart-type embryos to produce secondary embryos. Somatic embryos in liquid MS medium 110rpm 25 ℃ the dark culture 1w transfer to a Tony medium WPM 0.15 mg · L -1 IBA 30 g · L -1 < placed under strong light training / sup> sucrose g · L -1 agar 0.5 g · L -1 AC, the first low-light for 3 d, 1 w The left and right to seedling. 4 by Agrobacterium mediated Law, Wildvine stilbene synthase gene and its specific promoter containing vectors were transfected into tomato Micro-Tom, 51 transgenic seedlings were obtained by the preliminary detection of PCR and PCR-Southern. By high performance liquid chromatography of tomato transgenic plants and wild-type plants inoculated with Botrytis cinerea 24 h after resveratrol content determination, the results indicated the presence of transgenic plants with the highest content of resveratrol enhanced element Enhancer, is not to add any enhancement element vector gD2 into tomato 4.4 times the resveratrol content. Wild-type plants were not detected in resveratrol. The inoculated tomato gray mold disease index was measured, and the results show that the transgenic tomato have some resistance, which contain enhanced disease strongest element Enhancer enhanced vector transformed tomato.

Related Dissertations

  1. Polymorphisms in the Promoter Region of Swine BMP7 Gene and Their Association with Reproductive Traits,S828
  2. Cloning of Glyceraldehyde-3-phosphate Dehydrogenase Gene and Establishment of Agrobacterium-Mediated Transformation System of Rhizoctonia Solani,S435.111.42
  3. Identification of the Causal Organism of Soybean Bacterial Spots and Two Type Ⅲ Secreted Effectors’s Clone and Functional Analysis,S435.651
  4. Agrobacterium-mediated Transformation of GmWRKY21 into Soybean,S565.1
  5. Analysis of the Molecular Motif for Inducing Response to Ethylene and Jasmonic Acid in Pib Promoter Via Rice Transformation,S511
  6. Studies on the Induction of Pumpkin Chromosome Doubling and Diversification in Cytology and Physiology and Biochemistry of Somatic Embryogenesis in Pumpkin (Cucurbita Moschata D.),S642.1
  7. The Establishment of the Diagnostic Method for Detecting Antibody Against Avian Leukosis Virus Subgroup J and Function Analysis of the Long Terminal Repeat,S858.31
  8. Promoter Activity Analysis of Cytochrome P450 Gene CYP9A17v2 from Helicoverpa Armigera (H(?)bner),S435.622
  9. Molecular Diagnosis for Changes of Methylation of MLH1 Promoter of Arabidopsis Thaliana Induced by Cadmium Stress,X173
  10. Cloning and Function Analysis of P-ATPases Genes in Cotton and Tomato,S562
  11. In Vitro Culture of Zygotic Embryo in Quercus Liaotungensis Koidz.,S792.189
  12. The Prokaryotic Expression of Melittin Gene and Its Targeting Transcription in Hela Cells,R346
  13. The Investigation of Vimentin Exons and Promoter Regions in Age-related Cortical Cataract,R776.1
  14. Qingdao region of hepatitis B virus the BCP gene mutation and hepatitis B virus associated with primary liver cancer,R512.62
  15. Research on Subimage Selection and Mathching Method for Synthetic Aperture Radar(SAR) Target Recognition,TN957.52
  16. Expression Analysis of Storage Protein Avenin-like B Gene Promoter from Wheat (Triticum Aestivum),S512.1
  17. The Clone of Avenin-like B Gene Promoter and Construction of Expression Vector,S512.1
  18. Study of Lysyl-tRNA Synthetase Promoter,Q75
  19. Cloning and the Expression of PCD Related Genes of Somatic Embryogenesis in Longan,S667.2
  20. Studies on Biological Characteristics of Development and Regeneration of Zygotic Embryos in Hickory (Carya Cathayensis Sarg.),S664.1
  21. Modulation of IFN-γ-induced TRIM22 Expression by Histone Deacetylase Inhibitor TSA and the Underlying Mechanism,R346

CLC: > Agricultural Sciences > Gardening > Fruit trees gardening > Berries > Grapes
© 2012 www.DissertationTopic.Net  Mobile