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Sesame (Sesamum indicum L.) is part of the flax Division flax genus, widely grown in subtropical and temperate regions, rich in high quality protein, vitamins, fatty acids and amino acids, is the oldest oil crops. Phoma blight is one of the most serious fungal diseases sesame pathogen host range, resilience, coupled with the emergence of drug-resistant strains, the direct impact on sesame yield, quality, and mechanized operations. In recent years, the widespread use of fungicides, causing environmental pollution, pesticide residues, and ecological problems. Plant pathology researchers to explore the environment-friendly measures to prevent sesame Phoma blight, and long-term crop disease control Practice has proved that the use of crop disease resistance is an economical, safe, effective prevention and control measures. The purpose of this study seedling and adult stage anti sesame resources Phoma blight identification, screening resistant varieties, explore resistance resources, disease-resistant materials for Sesame breeding for disease resistance and production of direct application; second is cloned sesame Resistance Gene Analogs (RGAs), the Sesame anti Phoma blight related genes lay the foundation, or the RGAs developed molecular markers for molecular marker-assisted selection breeding. Currently achieved the following results: 1. Sesame seeds collected from different regions in Henan Province Phoma blight diseased isolated five pathogens observed by colony morphology the tieback identification and molecular detection to determine the Sesame Phoma blight pathogen Macrophomina phaseolina ( Tassi) Goid. Comprehensive comparison and analysis of the effect of different disinfectants determine disinfect 2% NaClO for 20 min, sterile water and then washed three times, the best disinfection effect. Henan 11 as a control material, identified by bacteria cake of 12 sesame germplasm resources of 5 Phoma blight pathogen seedling stage, the results showed that 12 copies of the resources of the different strains different type of reaction, which only ZZM0905 strong ZZM1205 resistance, and the resistance is relatively stable performance of high anti-or disease-resistant pathogens 5. Field nursery natural identification of the adult stage 74 sesame germplasm resources of Phoma blight resistance induced immune varieties were highly resistant varieties of 13 copies, 26 copies of resistant varieties susceptible varieties 29 did not find the highly susceptible varieties, ZZM0905 seedling and adult stage showed resistance to disease. Primers were designed based on the conserved domains of resistance genes, 26 anti Phoma blight and four copies of a sense of Phoma blight sesame genomic DNA as a template, PCR amplification of resistance genes homologous sequences. 128 sequence after sequence analysis of 42 sequence cloned RGAs non-TIR-NBS-LRR class sesame.
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