|
Wheat stripe rust is a fungal pathogens of wheat stripe rust (Puccinia striiformis f.sp tritici) one of the most important diseases caused wheat production, average years to rise to 20% -50% cut can cause more serious pop Year large losses, even resulting in crop failure. For a long time, the control of wheat stripe rust is mainly dependent on the cultivation and promotion of chemical control and disease-resistant varieties. Production practices in the loss of wheat varieties resistant to stripe rust now clear that Article rust races of the composition and the frequency change. The physiological races sporulation ability to affect its viability, capacity development and popular to some extent by stripe rust sporulation sporulation gene regulation. Therefore, through the analysis of sporulation genes then explore different kinds of sporulation, Breeding and control of rust-resistant varieties of wheat rust has important practical significance. Sporulation related genes in different physiological interspecific differences in the analysis have not been reported, sporulation related genes using molecular cloning techniques clone sequence differences, small species epidemic predicted meaningful. Different physiological races sporulation, using molecular cloning techniques in this article, in different physiological races of wheat stripe rust sporulation related genes cloned into PsCon1 stripe rust of wheat breeding process by analyzing different kinds PsCon1 The nucleotide sequence of the amino acid sequence and gene expression levels to reveal the causes of sporulation Souchong. The specific findings are as follows: 1. Article 33 (CY33), water 11-7 (Su11-7) in Article 32 (CY32) Hybrid46-8, water 11-3 (Su11-3), water 11-4 ( Su11-4), water to 11-5 (SU11-5), water 11-13 (Su11-13) cloned sporulation genes PsCon1, the cloned sequence is part of the nucleic acid sequence registered in GenBank PsCon1 gene, starting at the 83 bp highlighted in gray at the ends at 613, including three exons (79bp-132bp, 213bp-359bp and with 432bp in-494bp Figure 1-4) and two introns (133bp-212bp and 360bp- 431bp). 2.8 small species of nucleotide sequence and amino acid sequence are not significantly change, just individual sites mutated individual base mutation 45bp at bar 33 and Article 32, with the other 6 inconsistent population base, which in Article No. 33, 32 bases for the C the other strains water 11-7 Hybrid46-8, water 11-3, water 11-4, water 11-5, and water 11 -13 in this locus nucleotide is A. In addition, in 65bp, 310bp, 465bp, 495bp and 510bp at both a base mutations. 65bp at the base of water 11-3, while the other race are C; 310bp at Hybrid46-8, water 11-3, water 11-4 bases T, while the other small species are C ; 465 bp at the base of the water 11-7 T; 495 bp at the base of the water 11-3 G, while other species are T; 510bp at water 11-5, water 1-13 base for T while the other small species nucleotide C. Wherein exons exist only two base mutation, but the mutation of the nucleotide did not affect the amino acid sequence of the gene encoding, belongs to a nonsense mutation, a nucleotide mutation in the intron. 3.8 small expression of genes PsCon1 in amount there are significant differences, the the water 11-3 highest expression level after 12 hours inoculated lowest expression Hybrid46-8. Water 11-7 the water 11-3 highest expression level was the lowest in the 24 hours after inoculation.
|