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Effects of Progesterone Administration on Cognitive Capability and the Research of Relationship on the Expression of Astrocytes in Mice

Author: YinJing
Tutor: LiYunSheng
School: Tianjin Medical University
Course: Human Anatomy,Histology and Embryology
Keywords: Progesterone Y - type maze Cognitive impairment Astrocytes Glial fibrillary acidic protein
CLC: R965
Type: Master's thesis
Year: 2011
Downloads: 23
Quote: 0
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Abstract

Aim of the present study was to progesterone in ovariectomized mice (estrogen and progesterone missing) learning and memory and other cognitive abilities and improve the role and mouse parietal cortex of GFAP immunoreactive cell expression, progesterone, cognitive ability and astrocytes express three whether there is relevance in depth. The first part of the method: 60 female Kunming mice were randomly divided into five groups: sham-operated control group (Sham) and ovariectomized control group (OVX), progesterone low dose group (LP, 50mg/kg), progesterone in doses group (MP, 100mg/kg) and progesterone high dose group (HP, 200mg/kg), in addition to the sham control group, the mice in each group underwent bilateral oophorectomy. After 2 weeks, every five days of each group of mice were injected intraperitoneally with different doses of progesterone or saline for 90 days. The end of the administration, the application of Y-type electric maze test method to observe the changes of the mice learning and memory and other cognitive functions. Part II: the mice in each group decapitated sacrificed to remove brain tissue, paraffin embedded, sliced ??processing. Some mice immunohistochemistry assay parietal cortex GFAP expression and use the the MOTIC image analysis system to take photographs of GFAP morphological and quantitative analysis. Results of the first part: progesterone cognitive ability test results of each group of mice, the Y-type maze escape reflex ability tests: Compared with the sham control group, the control group of ovariectomized keep the two phases are obtained in learning and memory escape reflective learning ability decreased significantly (P lt; 0.05); compared with the control group of ovariectomized, progesterone, middle dose group remained in the memory stage escape reflex ability to learn has been a significant improvement (P lt; 0.05). Part II: progesterone on mouse astrocytes results, Determination of astrocyte marker GFAP expression: GFAP expression assay, ovariectomized group compared with the SHAM group mice, GFAP positive cell AOD and positive cells increased expression levels (P lt; 0.05), progesterone middle dose group and high dose group compared with ovariectomized mice, GFAP-positive cells the AOD and positive expression of cell area level lower (P lt; 0.05 ). Conclusion 1, estrogen, progesterone deficiency can cause adult female mice learning and memory and other cognitive dysfunction, long-term progesterone replacement therapy can improve cognitive abilities in ovariectomized mice. 2, in this experiment, progesterone and cognitive abilities measured study found that progesterone has a certain role to improve cognitive dysfunction stage. 3, the mechanism of action of progesterone on learning and memory and other cognitive abilities may be through its regulation of the expression of astrocytes regulate astrocyte cell processes and cell body itself hyperplasia and overexpressed, thus ensuring the mice improvement of cognitive function of the brain play a protective role. 4, progesterone, cognitive abilities and astrocyte GFAP expression among association.

Effects of Progesterone Administration on Cognitive Capability and the Research of Relationship on the Expression of Astrocytes in Mice

Abstract

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