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Study on the Anti-tumor Effect of a New Eight-copper Complex Emulsion

Author: GeZuoLei
Tutor: ZhangLiRong；XuXia
School: Zhengzhou University
Course: Pharmacology
Keywords: New eight-copper complex emulsion (N-Cu) anti-tumor MTT S180 ascites tumor vivo cell cycle
CLC: R96
Type: Master's thesis
Year: 2011
Downloads: 50
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Abstract


Objective:To study the anti-tumor effect of a new eight-copper complex emulsion (N-Cu) using MTT test in vitro and a Sarcoma180 tumor (S180) animal model in vivo, and flow cytometry.Methods:1.To determine the effects of new eight-copper complex emulsion (N-Cu) on proliferation of four human tumor cell lines, human colon cancer cells (HT29), human cervical carcinoma cells (Hela), human gastric cancer cells (SGC7901)and human esophageal cancer cells (EC9706) using MTT assay after 24 h. Then by statistics obtain the inhibition of tumor cells and the half inhibition concentration (IC50) of new eight-copper complex emulsion.2. To randomize the mice and inject the new eight-copper complex emulsion (N-Cu) by tail vein of low, medium and high concentrations in mice with S180 ascites-tumor, observing its effects on body weight, feed intake, abdominal circumference, ascites volume, life extension rate, blood biochemistry and major organs.3. To carry out cell cycle analysis by flow cytometry based on animal experiments in the previous chapter after 24 h.Results:1.The MTT result showed that N-Cu significantly inhibited the proliferation of the four tumor cell lines after 24 h. With increasing drug concentration, the inhibition was significantly enhanced. And when the drug concentration is 100.00μg/ml, the inhibitory rate reached 70% or more.The IC50s of N-Cu in HT29, Hela, SGC7901 and EC9706 cells after 24 h were 23.50±0.32,17.50±0.02,34.50±1.04, and 31.95±0.51μg/ml, respectively.2. The results in vivo showed that N-Cu had no significant inhibitory effect on body weight and food intake in mice. However, N-Cu significantly reduced the abdominal circumference and ascites of tumor-bearing mice, and prolonged life span without apparent side effects. Biopsy showed the liver in high dose group had significant improvement in mice.3. S180 cells treated with N-Cu showed an increase in G0/G1 phase and decrease in S phase. Cells were arrested in G0/G1 phase. Which the most significant effect is high dose group, the percentages of G0/G1 phase cells were increased to 76.1% and 72.3% in the high and middle dose groups, respectively. The difference was statistically significant (p<0.05).Conclusion:N-Cu significantly inhibited the proliferation of the four tumor cell lines. With increasing drug concentration, the inhibition was significantly enhanced. The results in vivo showed that N-Cu had better anti-tumor effects with no obvious side effects; S180 cells treated with N-Cu by flow cytometry showed that N-Cu with anti-S180 cell proliferation, an increase in G0/G1 phase and decrease in S phase. Cells were arrested in G0/G1 phase. That N-Cu is a broad development prospect of the anti-tumor drugs.

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