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Ethanol Precipitation Method of Different Human Serum Albumin Microspheres and Nanoparticles

Author: Sun
Tutor: GaoQiPin
School: Changchun University of Traditional Chinese Medicine
Course: Medicinal Chemistry
Keywords: Ethanol precipitation method Human serum albumin Ginsenosides Rb1 Adriamycin Fluorescein isocyanate Tremella polysaccharide
CLC: R283
Type: Master's thesis
Year: 2011
Downloads: 119
Quote: 0
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Abstract


Purpose: Preparating of Human serum albumin microspheres and nanoparticles of different methods and size optimum conditions, which are suitable for industrial production. Then load of ginsenoside Rb1, adriamycin, fluorescein isocyanate and Tremella polysaccharide to evaluate the methods.Method: Prepared different size microspheres and nanoparticles by ethanol precipitation method, adsorption and binding assay using different types of drugs loaded. Determinated drug loading of ginsenosides Rb1, adriamycin, fluorescein isocyanate by HPLC and Tremella polysaccharide by Spectrophotometer. Using dialysis, enzymatic degradation method and freeze-drying method study of ginsenoside Rb1 human serum albumin microspheres for drug release properties, in vitro degradation and storage technology.Result: Prepared 3 size ranges of particles by ethanol precipitation, " less 100 nm "," 400nm-1000nm "," 1μm-2μm ". And this method can load ginsenoside Rb1, adriamycin, fluorescein isothiocyanate and Tremella polysaccharide. Ginsenoside Rb1 and Tremella polysaccharides human serum albumin particles have higher drug loading, and adriamycin and fluorescein isothiocyanate human serum albumin particles have lower drug loading. Ginsenoside Rb1 human serum albumin microspheres have ability of controlled release and can be adjusted degradation time in vitro. After freeze-drying Ginsenoside Rb1 human serum albumin microspheres can be saved stably at 4℃for 6 months.Conclusion: Ethanol precipitation method suit to preparate nanoparticles or microspheres loaded Ginsenoside Rb1 and Tremella polysaccharide which have some characters such as good water-soluble and stable chemical properties and can be preparated in different size, drug-loading and degradation time. Ethanol precipitation method is not suitable for particles containing adriamycin and other drugs with active chemical groups which can destruct the structure of human serum albumin. For some drugs which like FITC with less water-soluble, this method also has some limitations.

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