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Open experimental bone defect seawater soaked infection after bone repair

Author: LiuMin
Tutor: ZhuQingSheng；ZhuJinYu
School: Fourth Military Medical University
Course: Surgery
Keywords: Open injury Bone defects Seawater immersion Infection Artificial materials Repair
CLC: R83
Type: Master's thesis
Year: 2011
Downloads: 15
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Abstract

The development of industry, agriculture, transport and production and construction, making a variety of open trauma, the increasing number of patients with bone defects, large bone defects and trauma in the clinical treatment of infection after a very difficult question, and post-traumatic tissue infections greatly affect bone defect repair. With the development of marine science and medical support needs of the military struggle for seawater soaked tissue injury study more and more, but seawater immersion infected limb large bone defect repair and functional reconstruction No reports fewer and seawater immersion large segment of bone defect preparation of animal models of infection. In this experiment, in this context, especially to a particular Fujian coast waters to carry out a comparative study of large bone defects after open trauma and seawater immersion infection material repair. First to carry out open traumatic bone defects seawater immersion and preparation of animal model of infection, comparing experimental research and clinical use at this stage, two kinds of artificial alternative materials to repair bone defects. The main purpose of this study is compared with seawater immersion exposure to infection and terrestrial natural soft tissue repair and material to repair large bone defects effect, to provide support for future marine business and medical support for military struggle. This study were the following four aspects: one, pioneered open the limb large bone defects seawater immersion infected animal canine model preparation, take the male or female dogs weight 10 ~~ 15kg, taken before the calf after anesthesia radial at the radial middle part made 2 cm open long bone defect, the seawater immersion injured limb bones defect infection, complete limb bone defect seawater immersion infection animal model. Second, to carry out the Fujian a specific waters limb openness trauma after seawater immersion infected bacteria and their antibiotic sensitivity of the preliminary investigation. The specific seawater immersion test group, the control group wound natural exposure, each two hours, and then the wound sterile bandage observe the wound three days later, the take secretions bacterial culture and drug sensitivity test, to determine the infection. And antibiotic drug sensitive test the preferred effective antibiotic treatment of bone defects and infection of the animal, to guide the early use of bacterial infections antibiotics after open trauma and seawater immersion empirical won the valuable time for the treatment of infection after open trauma and seawater immersion. Through animal testing, observed after seawater immersion infection surrounding skin and soft tissue repair of defects on the injured limb bones. Observed bone defects seawater immersion and wound healing of skin infections, and deep soft tissue repair and materials into stability. The fourfold table statistical processing, the chi-square test and analysis on the experimental group wound healing effects and material stability are worse than the control group. Conducted a study on the effect of artificial substitute materials placed in the bone defect repair after infection. Two bone defects seawater immersion infection, the animals were randomly grouped body (self-setting calcium phosphate calcium phosphate cement solidification of nano-hydroxyapatite (Nano-hydroxyapatite, n-HA) composite bone segment implantation and CPC, CPC) implantation. X-ray after 12 weeks, respectively, taking the the stump bone tissue pathology observed HE staining soft tissue, bone specimens fixed VG staining. Observed by X-ray and pathological fracture fragments and materials granulation tissue, fibrous tissue, cartilage callus and new bone hyperplasia, given the line of soft tissue pathology rating, and give the bone ends to callus growth classification. Using factorial analysis of statistical analysis obtained after 12 weeks between the test group and the control group, and nano-composite bone and CPC soft tissue pathology results have a statistical difference (P lt; 0.05), the test group and the control group, and nanocomposite bone and CPC bone ends callus growth levels were significantly different (P lt; 0.01), between grouped and materials without interaction (P gt; 0.05). The clinical results show: the visible Vibrio flora unique to the seawater according to the results of bacterial culture, pathogenicity healing observed by gross specimen seawater immersion group wound skin and soft tissue healing worse than natural exposure group; healing histopathological grade contrast The test group healing effects worse than the control group, the degradation of materials to repair CPC solidification, the effect of new bone ingrowth can nanobone; callus growth situation worse than the control group, the same test group growth significantly better the CPC material induced osteogenic in nano bone. An analysis of the experimental results, the following conclusions can be obtained: serious impact on wound healing study confirms the virulent Vibrio seawater immersion great harm, caused by infection, drug sensitivity test shows, ciprofloxacin, penicillin, levofloxacin on the bacteria, bacteria and Vibrio have a higher rate of sensitive before susceptibility results out, can be classified as empirical conventional medication use; seawater immersion the open after infection of the bone defect artificial materials to repair poor; open bone defect seawater immersion infection After the CPC the solidification implants than nano material bone implant restorative effects. Widely used two kinds of substitute materials, CPC repair and promote better bone growth, bone defects in the future a certain reference for the selection of materials bone. We first prepared seawater immersion extremity the open bone defects and infection in animal models, worthy of promotion.

Open experimental bone defect seawater soaked infection after bone repair

Abstract

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