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Study of Adverse Reactions about Oculentum Entering the Subconjunctival Tissue

Author: Wang
Tutor: ZhuYu
School: Zhengzhou University
Course: Ophthalmology
Keywords: Ointment Matrix Subconjunctival tissue Adverse reactions
CLC: R779.6
Type: Master's thesis
Year: 2011
Downloads: 2
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Abstract

Purpose ointment is widely used in the treatment of eye diseases, and to maintain a prolonged effective concentration of the drug in the conjunctival sac. Is made mainly by the pharmaceutical ingredient and excipient, excipient select different differences may lead to adverse reactions of the site of action of the ointment ocular surface or drug. In recent years, a lot of literature research pointed out that the ointment was applied to the lacrimal passage after support lacrimal duct can play and the role of prevention and treatment the lacrimal and then adhesions, but there have been reports of false passage into the eye the ointment through surgery or trauma conjunctiva, orbital tissue and eyelids, and lacrimal sac surrounding tissue, will cause a severe inflammatory reaction and treatment more difficult. The purpose of this experiment: To observe the different matrix of topical ointment into the subconjunctival tissue caused adverse reactions, filtering out the matrix degradation and absorption the ointment of less adverse reactions, and to provide the experimental basis for the correct selection of eye ointment for clinical work and play a guiding role. Materials and methods healthy and clean guinea pig animal packet 2-4 weeks of age 72, weight 120-150 g, of either sex, eye disorders and head and face abnormalities. Random number table is divided into three groups A, B, C, n = 24. Animal model produced hydrochloric oxybuprocaine the eyedrops line guinea pig ocular surface anesthesia under a microscope using a 1 ml syringe and No. 7 (22 G) needle A group of right eye above the subconjunctival injection to the vaseline, lanolin and liquid paraffin matrix of erythromycin ointment 0.2 ml of the top of the B group of the right eye subconjunctival injection of sodium hyaluronate and Carbopol matrix ofloxacin ointment 0.2ml C group as a control group, the right eyes above the ball subconjunctival injection of hydroxypropyl methylcellulose 0.2 ml. Observation project and the time point were observed immediately after the subconjunctival injection of three drugs drugs situation gathered subconjunctival; after injection, 1d, 3d, 7d, 14d, 21d, 28d observe the degree of swelling of the conjunctiva, 0,,, said: 0 for no obvious swelling, the flat elevated conjunctival edema, no wrinkles formation for conjunctival uplift, blepharophimosis area visible bulge for the conjunctiva bubbly, prominent palpebral fissure, the upper edge of the partially covered cornea; observation of the six time points after injection diffusion of the drug absorption and degradation; conjunctival histopathologic changes as well as the six time points. Statistical analysis Statistical analysis was performed using SPSS17.0 statistical software. Ordinal variables among the three groups the degree of swelling of the conjunctiva information Wilcoxon test was used to test a = 0.05, P lt; 0.05 difference was statistically significant. Results 1 three drugs injected into the local situation observed immediately after injection, A group of drugs the subconjunctival was yellow-white oily like; B group of drugs pale white translucent; C group of drugs in the form of liquid under the conjunctiva transparent. A group of the degree of swelling of the conjunctiva after the injection of three drugs: conjunctival swelling to the main change significantly, 14d 0 eye, 21 d, 28 d the conjunctival still see swelling, no significant regression. Group B: conjunctival swelling to the main 14 d conjunctival swelling is reduced significantly, significantly narrowed the scope of the 28 d conjunctival swelling completely subsided. Group C: conjunctival swelling to the main 7d the conjunctival swelling gradually subsided, 14d conjunctival swelling has completely subsided. Among the three groups the degree of swelling of the conjunctiva in 1d, 3d, 7d difference was statistically significant (P lt; 0.05). Drug diffusion and degradation of the absorption of group A: 3d after injection ointment, ointment gradually spread to the surrounding bulbar conjunctiva, but no significant degradation and absorption. Group B: from 3d, see the spread of the ointment to the bulbar conjunctiva below, and with the proliferation is gradually degraded and absorbed 28 d ointment has been completely absorbed. Group C drug each time point were no diffusion, 1d after injection began to be degraded and absorbed 14d has been completely degraded and absorbed. Histopathological observation group A: to see mainly neutrophil infiltration of inflammatory cells conjunctiva, including lymphocytes, the large number of fibroblasts and fibrous tissue hyperplasia; 21d, 28d fibrous tissue machine, inflammatory cells disappeared . Group B: see mainly neutrophil infiltration of inflammatory cells in the conjunctiva, peripheral vascular dilatation and congestion, only a small amount of fibrous tissue; 28 d conjunctival structure is basically back to normal. Group C: only a small amount of inflammatory cell infiltration in the conjunctiva 7d inflammatory cells disappear, no fibrous tissue proliferation, and 14d the conjunctiva structure back to normal. Conclusion petroleum jelly, lanolin, and liquid paraffin ointment into the tissue matrix will cause severe inflammatory reaction, and is not recommended for the wound tissue surface. Into the subconjunctival tissue matrix glass sodium and carbomer eye ointment caused only mild tissue inflammation that can play after support for lacrimal duct probing the lumen and prevent tissue adhesion role. Hydroxypropyl methyl cellulose does not cause a significant inflammatory response, but because of its low viscosity and is not used for the lacrimal passage postoperative injected into the tear duct.

Study of Adverse Reactions about Oculentum Entering the Subconjunctival Tissue

Abstract

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