Dissertation > Excellent graduate degree dissertation topics show

Pathogenesis and Treatment Strategies of T Cell Acute Lymphoma Leukemia

Author: YangYanPing
Tutor: WangGuanJun
School: Jilin University
Course: Internal Medicine
Keywords: Notch1 T cell leukemia CD25 NK cells IFN-γ
CLC: R733.7
Type: PhD thesis
Year: 2009
Downloads: 143
Quote: 0
Read: Download Dissertation

Abstract


Acute lymphoblastic leukemia (ALL) in all leukemia accounted for 12%, while T-cell acute lymphoblastic leukemia (T-ALL): 15% of children with ALL, accounting for 25% of adult ALL. Through chemotherapy, hematopoietic stem cell transplantation, there are still 20-25% of children develop into a poor prognosis refractory leukemia and adult patients, the 3-year survival rate is only 35-40%. Andrew et al in 2004 first proposed more than 50% of human T-ALL activation of Notch1 mutant Therefore Notch1 become recognized the pathogenesis of T-ALL oncogenes. Explore the relationship between Notch1 and T-ALL pathogenesis will further guide the clinical treatment of T-ALL. Pathogenesis: Notch1 transduction of T-ALL leukemia cells in mice subculture, closer to the clinical phenotype and functional T-ALL model was established for the first time. Phenotype characterized by expression of CD 4 , part of the expression of CD25, highly TCRVβ8.1/8.2 rearrangement, low expression of MHCI. Its features: not survive under normal culture conditions in vitro, but can survive under ConA or rhIL-2 conditions. This study is the first Notch1-transduced T-ALL leukemia cells are divided into different expression levels of CD25, CD25 and CD25-2 class of cells in normal mice in vivo distribution in these two types of cells and caused mortality fewer CD25-cells infiltrating the spleen and lymph nodes, with faster mortality. CD25 cells can be converted into CD25-cells, CD25-cells into CD25 cells rarely, but in vitro rhIL-2 cultured under the conditions, the CD25-Notch was able to complete transformation into CD25 cells. EL4 lymphoma cell lines further evidence of the CD25-cells with faster mortality CD25 EL4 EL4 in Multi Power in the body can differentiate into CD25-CD25-the EL4 EL4 in Multi Power can not differentiate into CD25's. Therapeutic strategy: The study was first proposed in vivo tests, syngeneic NK cells after removal of the survival of leukemic mice was significantly shorter, and the CD25 cell lethal rate faster than the lethality rate of the CD25-cells, CD25-cells are able to differentiate CD25 cells. Normal NK cells, anti-CD25 leukemic cells, inhibition of CD25-leukemia cells to CD25 changes. The same time be able to reduce the CD25 tumors lethality. Allogeneic hematopoietic stem cell transplantation can improve the leukemia survival time. IFN-γ can not through its receptors play an indirect role in killing tumor.

Related Dissertations

  1. TRAIL in the regulation of tumor invasion CD4 ~ CD25 ~ Treg,R730.2
  2. The Influence in Different Route of Medication with Vitamin A on CD4~+CD25~+ Regulatory T Cell and Cell Factor of IL-4、 IFN-γ in a Rat Persistent Asthma Model,R562.25
  3. Effects of Vitamin A and Montelukast on Airway Inflammatory Cells and Th1/Th2 Balance in Mouse Model of Asthma,R725.6
  4. The Clinical Significance of T Cell Subgroups and NK Cell’s Expression Level in Diffuse Large B-cell Lymphoma Patients,R733.1
  5. Exploreing the Mechanism of Xieshifeiaifang Ii on Treating Lung Cancer with Pleura Effusion,R734.2
  6. Establishment of Stable Subline of K562 Cells Overexpressing HMGB1 Protein.,R733.7
  7. Diagnosis Value of Cytokine Levels and TB-Ab in Serum in Children Tuberculosis and Latent Tuberculosis Infection,R725.1
  8. The Expression of CD4+CD25+ Regulatory T Cell、TGF-β1 on Peripheral Blood from Patients with Alopecia Areata,R758.71
  9. Bone marrow mesenchymal stem cells in rats with severe acute pancreatitis CD4 ~ CD25 ~ regulatory T cells influence and Integrative Medicine,R576
  10. Bone marrow mesenchymal stem cells in the same kind of heart transplantation on Treg cells in rats,R654.2
  11. The Distribution of KIR Gene in Chinese Han Population and the Role in Unrelated Allogeneic Hematopoietic SCT,R392
  12. The Change and Significance of CD4~+ CD25~+ T Regulatory Cells in Children with Primary Nephritic Syndrome,R726.91
  13. The Expression of IL-22 in the Early Stage of Murine TNBS Colitis,R574.62
  14. Phenotypic and Functional Study of NK and γδT Cells in Malignant Pleural Effusion,R730.4
  15. Investigate the Levels of CD4~+CD25~+CD127~- Regulatory T Cells in Peripheral Blood of Patients with Cervical Carcinoma,R737.33
  16. Difference in the Expression of Notch-1 Gene during Muscle-derived Stem Cells Repairing Spinal Cord Injury in Rats,R329
  17. The Induction of Proteins ESAT-6 and Ag85B Secreted by Mycobacterium Tuberculosis on FoxP3mRNA in Peripheral Blood,R392
  18. Sirolimus Induces Rat Na(?)ve CD4~+ T Cells to Regulatory T Cells and Its Application,R965
  19. Notch1 and Its Ligand of JAG1 Gene Methylation Role of Onset in Mammary Gland Carcinoma and Difference between Uygur and Han Women in XinJiang,R737.9
  20. Interactions Between Bm-mesenchymal Stem Cell from Acute Leukemic Children and Natural Killer Cell from Cord Blood: Evidence in Vitro and Vivo,R733.71
  21. Expression of Notch1 in Cervical Cancer Tissues and Its Effect on Invasion and Metastasis of Cervical Cancer Hela Cells,R737.33

CLC: > Medicine, health > Oncology > Hematopoietic and lymphoid neoplasms > Leukemia
© 2012 www.DissertationTopic.Net  Mobile