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Objective: To observe the dynamic expression of miR-146a and tumor necrosis factor - α ( TNF -α ) in lipopolysaccharide (LPS) -induced alveolar macrophages , analysis of the changes in the relationship between both time to explore miR-146a alveolar the regulatory role of the macrophage inflammatory response and its mechanism . : NR8383 alveolar macrophages in vitro 1 × 106 cells / mL were seeded in six-well plates , adherent after 90min joined 1μg/mL of LPS, stimulus 0h , 3h , 6h , 12h after centrifugation culture supernatant was collected and cell clumps . The real-time quantitative PCR (RT-qPCR) to detect the expression of miR-146a and TNF-αmRNA , cells , the enzyme-linked immunosorbent assay (ELISA) in the culture supernatant levels of TNF-α protein expression . miR-146a and TNF-αmRNA , , bivariate Pearson correlation analysis . Results : 1.LPS 6h after stimulation of the cells in the expression levels of miR-146a began to increase (5.33 ± 0.81 -fold , P lt ; 0.01) and sustained (12h: 8.21 ± 1.19 -fold higher , P lt ; 0.01) ; TNF-a mRNA expression in the cells that peaked after LPS stimulation 3h , (67.48 ± 24.52 -fold , P lt ; 0.01) 6h after (29.53 ± 4.26 -fold lower , P lt; 0.05 ) ; 3 culture supernatant 3h after elevated ( land (359.80) ± 57.54pg/mL in TNF-α protein in LPS-stimulated P lt ; 0.01) , and reached the peak at 12h ( 729.22 ± 50.40pg/mL P lt ; 0.01) ; 4 cells the expression levels of miR-146a with TNF-αmRNA content was negatively correlated ( r = -0.895 , P lt ; 0.01) . Conclusion : LPS stimulation 0-12h , miR-146a , and TNF-α expression presents a dynamic change , and the cells in the expression level of miR-146a was negatively correlated with TNF-αmRNA content speculate that miR-146a may be involved alveolar macrophage inflammatory response .
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