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The Effects of Endothelial Microparticles on Monocyte Associated Angiogenesis

Author: WangShiKun
Tutor: TianZuo
School: Shandong University
Course: Human Anatomy and Embryology
Keywords: Endothelial microparticles Monocytes Endothelial cell proliferation
CLC: R329
Type: Master's thesis
Year: 2011
Downloads: 43
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Abstract

Background: endothelial microparticles (Endothelial microparticles, EMPs) are endothelial cells metabolism, activation or apoptosis when secreted into the extracellular space and body fluids vesicle-like substance. In recent years, research has been for many endothelial microparticles. Jy W, et al experiments confirmed: in multiple sclerosis, through the activation of endothelial monocyte particles so that more monocytes in inflammation and the formation of multiple sclerosis. In Sabatier F et al, found: endothelial microparticles can induce monocytes tissue factor (Tissue factor, TF), trigger TF-dependent procoagulant response. However, endothelial microparticles on monocyte participation angiogenesis has not been reported at home and abroad. Objective: To study on monocyte endothelial microparticles in angiogenesis effects, including studies of endothelial microparticles on monocyte-mediated endothelial cell proliferation and induce the endothelial microparticles monocytes to endothelial cell transdifferentiation possibilities. Methods: In vitro cultured human umbilical vein endothelial cells (Human umbilical vein endothelial cells, HUVECs), ultracentrifugation supernatant of endothelial microparticles precipitated, resuspended in sterile PBS; TEM identification of endothelial microparticles morphology and structure; the endothelial particles and THP-1 cells were cultured using PCR and ELISA, cell lines THP-1 VEGF-A, VEGF-C expression; the HUVECs and the original monocytes or transendothelial activated monocytes particle discharge Transwell separated into small indoor cultivation, with WST-1 assay kit HUVECs proliferation conditions; using PCR and immunofluorescence observed after activation of endothelial microparticles THP-1 cells to endothelial markers vWF and VEGFR2 expression. Results: TEM, endothelial microparticles as low electron density spherical diameter between the 100-1000nm, clear boundary, surrounded by a high electron density phosphotungstic acid; compared with the control group, endothelial microparticles stimulated THP -1 cells within the VEGF-A and VEGF-C protein and mRNA levels were elevated; proliferation assays showed that both of the original culture or mononuclear cells and endothelial microparticles activated monocytes were cultured, HUVECs were significantly increased, while the fine particles and endothelial cells activated monocytes were cultured HUVECs ratio of the original monocytic cells was cultured HUVECs proliferation; were cultured endothelial microparticles after 3d, THP-1 cells and VEGFR2 expression of vWF positive, negative expression of vWF and VEGFR2 after cultivation 8d protein and gene expression were negative. Conclusion: The endothelial microparticles induce monocytes by VEGF-A and VEGF-C to promote proliferation of endothelial cells; endothelial microparticles only the monocytes showed a transient endothelial phenotype, which can not differentiate into endothelial cells. Significance: This study wound healing, tumor metastasis and other physiological and pathological processes of angiogenesis provides new mechanism for the treatment of cardiovascular disease provides a new perspective on the field of angiogenesis Other researchers have some reference value. In addition, we carry on endothelial microparticles part of the analysis of mRNA for endothelial microparticles in other studies provide a basis for the application.

The Effects of Endothelial Microparticles on Monocyte Associated Angiogenesis

Abstract

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