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Objective: To learn by observing the behavior of the CUMS change and the hippocampus in the 5 - HT, 5 - HIAA, dopamine and norepinephrine content, Ukraine gland Hypericum possible antidepressant effect mechanism. Methods: 50 female Wistar rats were randomly divided into five groups, the blank group, model group, high-dose treatment group Wu gland Hypericum, Wu gland Hypericum low-dose treatment group, positive drug group. In addition to the blank group, each rat solitary raising and accept stimulus stressors, including fasting for 24 h, the water deprivation 24h, 40. C high temperature 5min, 4 ℃ ice water swimming 7min, electric shock plantar 5min, tail clamp 5min, day and night reversed 24h of 7 stimuli were randomly assigned to 21 days, and daily, each stimulus appears three times, not to make the same kind of stimulus consecutive, to prevent the animals expected stimulation occurring. Eighth day administered once daily, Ukraine gland Hypericum high dose group were given 2.88g/kg, the low-dose group were given 0.72g/kg positive drug group were given fluoxetine hydrochloride 1.8mg / kg. 21 days after the observed weight changes in rats before and after modeling, and decapitated, hippocampus, measured by ELISA and 5 - hydroxytryptamine (5-HT), 5 - hydroxyindoleacetic acid (5-HIAA), dopamine (DA ) and norepinephrine (NE) content. Results: 1. Changes in body weight performance: seven days, compared with the control group, the weight of depression group, a significant difference (p lt; 0.05), depression group, weight gain was a significant difference (p lt; 0.01). After 21 days, compared with model group, the drug group was not statistically significant (p gt; 0.05) in body weight. Positive group significant difference (p lt; 0.05), low-dose and high-dose groups, although no significant differences, but the increase in body weight of rats have a role in weight gain. 2. Behavior in rats (Open-Field) performance: 21 days, compared with the model group, drug rats vertical activities and the level of activity increased significantly, there is a significant difference (p lt; 0.05). The experimental table Mingwu gland Hypericum can significantly improve the rats of depression caused by the decrease of the number of activities. Sucrose consumption (preference) performance: seven days, the sucrose preference of the depression group was significantly lower than the control group (P lt; 0.01), and lasted 21 days. 21 days, compared with the model group, drug group sucrose preference very significant difference (P lt; 0.01). The experimental table Mingwu gland Hypericum can be significantly decreased sucrose preference reversal CMUS depressive rats. 4.5-HT ,5-HIAA, DA, NE content changes in performance: 21 days after modeling, model group, 5-HT and 5-HIAA, DA and NE content of the corresponding blank group were significantly lower. Compared with the model group, Wu gland Hypericum high dose group and positive group of 5-HT content significantly higher (p lt; 0.01); 5-HIAA content of the high-dose group and positive group was significantly higher (p lt; 0.05 ); DA content of low-dose, high-dose group and positive group had no significant change (p gt; 0.05); the NE high dose of positive group were significantly improved p lt; 0.05). Conclusions: 1. Solitary dependents with chronic stimulation unpredictability make the model rats weight and sucrose consumption, Open-Field number of significantly reducing to objectively reflect depression in rats, with clinical manifestations of patients with depression very similar, corroborated the CUMS model of depression copy success. Ukraine gland Hypericum weight gain on depression in rats is not statistically significant; drug group depression in rats can be sugar consumption and increase in the number of activities. The experimental table Mingwu gland Hypericum can to some extent improve the performance of the depression of the depression in rats. Solitary dependents with chronic stimulation unpredictability of the model group rat 5-HT ,5-HIAA, NE decreased, suggesting that changes in brain monoamine neurotransmitters content related to the occurrence of depression, and depression consistent with the hypothesis of a single amine. High dose group can make depression hippocampal 5-HT and 5-HIAA, NE content corresponding increase may be one of the mechanisms of its antidepressant effect.
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