Dissertation > Excellent graduate degree dissertation topics show

Hydrogen-rich Saline and Hyperbaric Oxygen Preconditioning Ameliorates Intestinal Ischemia-Reperfusion Injury in Rats

Author: ChenHan
Tutor: WangQiang；SunXueJun；SunYanPing
School: Second Military Medical University
Course: Surgery
Keywords: Intestinal ischemia-reperfusion Hydrogen Hyperbaric oxygen Oxidative Stress Apoptosis
CLC: R656.7
Type: PhD thesis
Year: 2010
Downloads: 234
Quote: 0
Read: Download Dissertation

Abstract

Background intestinal ischemia-reperfusion injury is common in abdominal aortic aneurysm surgery, small bowel transplantation, cardiopulmonary bypass, intestinal strangulation, neonatal necrotizing enterocolitis, also occurs in septic shock and hypovolemic shock. Intestinal ischemia-reperfusion injury will affect not only the function of intestinal absorption, it will lead to the damage of intestinal mucosal barrier function, resulting in intestinal bacteria and endotoxin into the systemic circulation, caused by the reticuloendothelial system is a series of reactions, leading to a large number of medium and the release of cytokines, and even induce systemic inflammatory response syndrome (Systemic inflammatory response syndrome, SIRS) and multiple organ failure (Multiple organ failure, MOF), therefore the small intestine is known as the origin of the \The second hit of the origin. The generation of reactive oxygen species is an important mechanism for intestinal ischemia-reperfusion injury. Reperfusion produce an excess of oxygen free radicals, oxygen free radicals, the main target of the lipids, proteins, nucleic acids and other biological macromolecules. The cell membrane, mitochondria film membrane structures rich in unsaturated fatty acids, are particularly vulnerable to oxygen radical attack, resulting in lipid peroxidation damage biofilm, causing cell necrosis or apoptosis. The hydrogen is considered to be a novel anti-oxidant having selective anti-oxidation. The hydrogen applied basic research in the field of disease prevention and control in recent years in the ascendant, and play an important role in many diseases and injuries of the brain, heart, liver ischemia, diabetes, intestinal transplantation injury, chronic hepatitis, arterial atherosclerosis and other. Recent studies have reported that the animal breathing 2% hydrogen can effectively scavenge free radicals, a significant improvement in cerebral ischemia-reperfusion injury, and the chemical reaction, cytology means prove, hydrogen dissolved in the liquid can be selectively and hydroxyl radical and nitrite anion, the latter two are important mediators of oxidative damage, the body lacks its metabolic pathways. Hyperbaric oxygen is widely used to treat carbon monoxide poisoning, decompression sickness, arterial gas embolism, and as adjunctive therapy means the treatment of diseases of various oxygen supply obstacles. Hyperbaric oxygen preconditioning mostly used for ischemic tolerance of the brain, spinal cord, liver and cardiac research results that can significantly reduce the brain, spinal cord, liver, and myocardial ischemia-reperfusion injury. Hyperbaric oxygen preconditioning can stimulate endogenous protective mechanism, so as to reduce tissue damage caused by the by subsequent tissue ischemia, trauma, and other factors play a better role in the prevention, more valuable in clinical applications. Currently, hyperbaric oxygen preconditioning effect on intestinal ischemia-reperfusion injury is not yet clear. Goal 1 by constructing intestinal ischemia-reperfusion model of intestinal ischemia-reperfusion injury, validity, and preliminary mechanism of observed hydrogen water treatment. 2. Research hyperbaric oxygen preconditioning on intestinal ischemia-reperfusion injury, and to investigate the mechanism mediated effects. Method 1. Superior mesenteric artery noninvasive temporarily blocking 45min, 60min of reperfusion after restoration of mesenteric blood flow to build intestinal ischemia-reperfusion injury model. Reperfusion 60min, fast clipping the 0.5cm long jejunum ring, wash away the contents placed in vitro tissue perfusion bath for intestinal tension measurement. Take rat distal jejunum prepared sample of intestinal tissue homogenates was measured sample malondialdehyde content and myeloperoxidase activity. Serum cytokine levels detected by double antibody sandwich ELISA. 5.TUNEL assay apoptosis of intestinal epithelial cells. 6 Immunohistochemical detection of PCNA expression and distribution of intestinal epithelial cells. 1 first part: with ischemia-reperfusion group and ischemia-reperfusion saline group contrast, hydrogen water rats intestinal response to KCl was significantly higher (P lt; 0.01), prompt reperfusion 30min before via the tail the intravenous hydrogen water (6 ml / kg) can significantly improve the ischemia-reperfusion-induced intestinal contractile function decline. Group compared with sham surgery, ischemic reperfusion group and ischemia-reperfusion the saline rats intestinal tissue MDA content, MPO activity, serum TNF-α and IL-6 levels increased significantly (P lt; 0.05), while the hydrogen treatment can significantly reduce rat intestinal tissue MDA content, MPO activity and serum TNF-α and IL-6 levels (P lt; 0.05). Hydrogen water rats intestinal crypt expression of PCNA-positive cells was significantly higher than the ischemia-reperfusion group and ischemia-reperfusion the saline group level (P lt; 0.01), 30min before reperfusion by intravenous injection of hydrogen water (6 ml / kg) can significantly promote the proliferation of intestinal crypt epithelial cells. Sham group rat intestinal epithelial apoptotic cells rarely. Compared with the sham group, ischemia-reperfusion group and saline group ischemia-reperfusion rat intestinal epithelial TUNEL-positive cells was significantly increased (P lt; 0.01). And hydrogen group rat intestinal epithelial TUNEL-positive cells was significantly lower than the ischemia-reperfusion group and ischemia-reperfusion the saline group level (P lt; 0.01) 30min before reperfusion by intravenous injection of hydrogen water (6 ml / kg) significantly inhibited ischemia-reperfusion injury induced intestinal epithelial cell apoptosis. Second part: with ischemia-reperfusion group contrast, rats pretreated with bowel hyperbaric oxygen reactivity to KCl was significantly higher (P lt; 0.01), suggesting that hyperbaric oxygen preconditioning can significantly improve ischemia reperfusion-induced intestinal contractile function decline. In contrast to the sham group, the MDA content and MPO activity of rat intestinal ischemia-reperfusion group was significantly higher (P lt; 0.05), and hyperbaric oxygen preconditioning can significantly reduce rat intestinal tissue MDA content and MPO activity (P lt; 0.05). Sham group rat intestinal epithelial apoptotic cells rarely. Compared with the sham group, ischemia-reperfusion group rat intestinal epithelial TUNEL-positive cells was significantly increased (P lt; 0.01). Hyperbaric oxygen preconditioning group rat intestinal epithelial TUNEL-positive cells was significantly lower than the ischemia-reperfusion group level (P lt; 0.01), hyperbaric oxygen preconditioning significantly inhibited ischemia-reperfusion injury induced by intestinal epithelial cells withered perish. Conclusion 1. Hydrogen a significant protective effect on intestinal ischemia-reperfusion injury, its mechanism may inhibit intestinal ischemia-reperfusion induced oxidative stress injury, inflammatory response and intestinal epithelial cell apoptosis, promote intestinal epithelial cell proliferation. Hyperbaric oxygen preconditioning may be through its antioxidant, anti-inflammatory, anti-apoptotic effect so as to reduce intestinal ischemia-reperfusion injury.

Related Dissertations

CLC: > Medicine, health > Surgery > Of surgery > Abdominal surgery > Small intestine
© 2012 www.DissertationTopic.Net Mobile