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The Effect of Dietary Enegy on Growth Performance and MSTN Gene Expression in Landes Geese and Xupu Geese

Author: HuJunPeng
Tutor: HeRuiGuo；XiongYuanZhu
School: Huazhong Agricultural University
Course: Animal Genetic Breeding and Reproduction
Keywords: Energy Landaise Xupu goose Early Growth GH IGF-I Myostatin gene Expression levels
CLC: S835
Type: PhD thesis
Year: 2008
Downloads: 360
Quote: 2
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Abstract


Landes goose and Xupu goose is the major domestic varieties of goose liver. Strains cultivation is an important aspect of the development process of industrialization of foie gras goose. Most studies show that dietary ME levels of the early goose weight gain than the protein level, but few researchers specific research on the energy of a factor early growth and development of foie gras goose strains. The subject by changing the metabolic combination of the Landes goose and Xupu goose early growth situation, the key day-old carcasses indicators related blood biochemical parameters and serum hormone levels and muscle generates inhibiting factor (MSTN) gene expression levels and other factors. Explore the the Landes goose and the Xupu goose early growth and development of ultra-feeding pre appropriate ME nutritional needs and related laws involving early growth. The test results are summarized as follows: test 1 0 ~ 70d stage energy growth and development of the Landes goose gander Objective: To study the growth performance of the different levels of dietary energy the Landes goose gander ultra-feeding pre (0 ~ 70d) carcass performance indicators of body size, blood biochemical parameters affect hormone levels and muscle composition. Methods: This study used 90 0-day-old newborn material caused by the same Landes goose gander test object design by adding soybean oil to high, medium and low three different metabolic energy level (0 ~ 3w: 13.38MJ/kg, 12.13MJ / kg and 10.87MJ/kg of; 4 to 10 w: 13.81MJ/kg 12.55MJ/kg and 11.30MJ/kg) diet trial geese were randomly divided into test group Ⅰ, group Ⅱ, group Ⅲ, each The 30 columns fed for 70 days. The week weighed and measured body size; slaughter test 21d, 70d, and collected blood serum parameters and serum hormone levels detected before slaughter; muscle composition measured after slaughter. Results: (1) appropriate Landes goose gander energy level: 0 ~ 21d 12.13MJ/kg 22 d to 70 d 12.55MJ/Kg; (2) Dietary energy for the the Landes goose gander slaughter rate, eviscerated rate, the the eviscerated rate, leg muscle rate differences were not significant (p> 0.05); (3) the Landes goose gander muscle protein content of over 21%; breast and leg total lipid content with the dietary energy concentration increase and improve the group I pectoral muscle and leg muscle total lipid content than group Ⅲ were 24.22% and 34.73%; higher than the leg muscle pectoral muscle in the various chemical ingredients; (4) 21d, Landes geese public goose serum TG, LDL, HLDL difference was not significant (p> 0.05); the 70d when the Landes goose gander serum AST, LDL and HLDL content difference was not significant (p> 0.05); (5) 21d serum IGF- Ⅰ measured value to except (♂ Ⅲ) were higher than the measured value 70d groups; the ME level affect the level of serum IGF-Ⅰ 21d significant difference (p <0.05); 21d serum GH measured values ??with the increase of dietary energy while elevated 70d gander groups GH difference was not significant (p> 0.05). Objective: To study the growth and development of test 2 0 ~ 70d stage energy Landes goose goose different dietary energy levels of the Landes goose goose feeding pre (0 ~ 70d) growth performance, carcass performance, body size indicators, blood biochemical parameters, hormone levels and muscle composition affect. Methods: with Landes goose gander. Results: (1) appropriate Landes goose goose energy levels: 0 ~ 21d 13.38MJ/kg, 22 ~ 70d for 13.81MJ/Kg; (2) set of the goose leg muscle in dry matter content were higher than the same group pectoral muscle dry matter content of about 4.58%, 0.21% and 10.29%; pectoral muscle total lipid content were higher than the same group of leg muscle total lipid content of about 1.53%, 6.91% and 1.88%; (3) 21d, Long Germany goose goose TG, LDL, HLDL difference not significant (p> 0.05); 70d, Landes goose goose serum AST, LDL, TC, AKP and HLDL the content of the difference was not significant (p> 0.05). (4) 21d determination of serum IGF-Ⅰ values ??were higher than 70d measured value; 21d Goose IGF-Ⅰ groups had no significant difference (p> 0.05), 70d significant difference (p <0.05). 21d goose GH increased with the improvement of dietary energy. Growth and development of test 3 0 ~ 70d stage energy Xupu goose Objective: To study the different dietary energy levels of the Xupu geese feeding pre (0 ~ 70d) growth performance, carcass performance indicators of body size, blood biochemistry indicators, the impact of hormone levels and muscle composition. Methods: with geese gander. Results: (1) Suitable Xupu goose energy levels: 0 to 21d energy level 13.38MJ/kg 13.81MJ/Kg 22 to 70D energy levels. (2) the feed medium energy Xupu goose Ⅱ group on five indicators in the slaughter rate, eviscerated yield, eviscerated rate, percentage of abdominal fat and breast muscle rate and the remaining difference was significant (p <0.05); For subcutaneous fat thickness and muscle the Xupu goose Ⅰ, Ⅱ and group Ⅲ group the difference between the two indicators of fat thickness significantly; abdominal fat weight in group II (p <0.05) and abdominal fat, high in the other two groups, the difference was significant (p <0.05). (3) feeding different energy diet the Xupu goose breast and leg protein content differences are not significant (p> 0.05); 4.46% and 8.66% of the total lipid content in the I group pectoral muscle Ⅱ, Ⅲ group pectoral muscle total lipid content ; I set of leg muscles in the total lipid content than the II group III thigh muscle total lipid content of 4.99% and 11.63%, respectively. (4) when the 21d and 70d, Boxu the Xupu goose serum IGF-I measured value differences not significant (p> 0.05); Determination of serum GH values ??below 21d 70d when the measured value. Test 1-3 conclusions: (1) Landes goose gander the Landes goose goose and Xupu goose early appropriate ME levels: 0 to 21d, respectively 12.13MJ/kg, 13.38MJ/kg and 13.38MJ/kg; 22 ~ 70d, respectively 12.55MJ/kg, 13.81MJ/kg and 13.81MJ/kg; (2) of the trials, dietary energy levels can affect a goose body size and development; (3) ME levels were significantly affect Landoise part slaughtering indicators (male goose carcass weight, whole eviscerated weight, eviscerated weight, breast muscle weight, leg muscle weight and abdominal fat weight); dietary energy levels on Landoise dressing percentage, eviscerated yield, semi-eviscerated rate leg muscle rate the impact of the differences were not significant; (4) ME level extremely significantly affect the the Xupu geese slaughter rate, eviscerated yield, half eviscerated rate, percentage of abdominal fat and breast muscle rate of five indicators; (5) Long Germany goose muscle crude fat content than the Xupu Goose muscle crude fat content; same energy levels Landes gander crude protein, crude fat and crude ash content higher than that of the Landes goose; the the ME level will affect the muscles chemical composition; (6) GH levels with ME to improve and improve; no correlation between the level of serum IGF-Ⅰ and dietary energy levels. Test 4 Landoise generated by the xupu goose muscle inhibition factor (MSTN) gene cloning and energy of their expression levels affect research purposes: use of reverse transcription - polymerase chain reaction (RT-PCR) technology, cloning Landes goose , generated by the xupu goose muscle inhibition factor (MSTN) gene to study the relationship MSTN gene expression and dietary energy content of some serum hormone understand the MSTN functionality to provide basic data for the waterfowl molecular nutrition and molecular breeding. Method: extraction of total RNA from the goose, xupu goose the leg muscle using a two-step RT-PCR amplified the the MSTN gene cDNA coding sequence for the vector pGEM-T Vector, the fragment was cloned into the large intestine bacilli (Escherichia coli). The positive clones were screened the double digestion after sequencing; MSTN gene cloning based on β-actin as an internal standard, semi-quantitative RT-PCR method to build optimized study three different energy Landes goose gander the the Landes goose goose and Xupu goose in the 21d and 70d two periods of muscle tissue MSTN gene expression differences; same time with 21,70 d serum GH was measured by radioimmunoassay of IGF-I concentrations. Results: (1) cloning to goose, the Xupu goose MSTN gene cDNA partial sequence, 1128bp fragment size, which encodes a 375 amino acid polypeptide composed of MSTN nucleotide similarity of chickens, ducks, geese and published were 94%, 94%, 99%; similarity of the amino acids were 98%, 97% and 98%; (2) 21d, Landes goose gander, goose and the Xupu goose MSTN expression level of the difference was not significant; (3) 70d the Landes goose gander MSTN expression levels of Ⅰ> Ⅲ> Ⅱ, the Landes goose goose MSTN expression levels III> Ⅱ> Ⅰ, Xupu goose MSTN expression levels Ⅲ> Ⅱ> Ⅰ; (4) dietary energy 21d after Landoise, the Xupu goose MSTN gene expression influential MSTN gene expression levels and serum IGF-Ⅰ trends are basically the same;-free and serum GH content correlation was positively correlated with the level of GH and energy. Conclusion: Dietary energy 21d after Landoise, the Xupu goose MSTN gene expression influential MSTN gene expression and serum levels of IGF-Ⅰ, and serum GH correlation.

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CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Poultry > Goose
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