Dissertation > Excellent graduate degree dissertation topics show

Effects of IGF-2 on Plasma Metabolite and Hormone levels in Pregnant Animals and Their Fetuses during Late Gestation

Author: ChenQiXin
Tutor: MaoZuoZhi;YangLiGuo
School: Nanjing Agricultural College
Course: Animal Genetic Breeding and Reproduction
Keywords: IGF-2 fetuses sheep rats hormone metabolism cholic acid
CLC: Q943.99
Type: PhD thesis
Year: 2000
Downloads: 194
Quote: 3
Read: Download Dissertation


To evaluate the roles and mechanism of IGF-2 on fetal growth and development during late gestation, pregnant ewes and rats were employed to investigate relationship between IGF-2 and metabolites, hormones of pregnant animals and their fetuses. The dynamic variations in the plasma IGF-2, and its correlation with plasma hormones and metabolites in pregnant ewes with their fetuses and neonates as well as the growth and development of fetuses and neonates during perinatal period at normal condition and series of experiments (with IGF-2 fragment, glucose, insulin and cholic acid ) have been measured. The results showed as follows: 1. Relationuhlpbetwntheplauum IGF?and.utabolisu, horu,on.s lnpregnunt sheep durIng late gestation 6 comdale pregnant ewes were randomly allotted into CA group and control group after 2 weeks of adaptation. Before treatment, jugular vein catheters were installed. CA-treated group was infused with 2mg.kg~?cholic acid through jugular vein catheter and control group was administrated with the quantitative 0.9% saline. The next infusion was conducted on day 14 after front infusion until parturition. Blood samples were collected daily before and after infusion for assaying levels of plasma IGF-2, other hormones and metabolites, and calculating the correlation among them, as well as studying the effects of cholic acid loading on them. The results showed that the plasma IGF-2 levels in late gestational ewes varied between 36.25 ?1 .O2ng/ml -61.18 ? 1 3.6SngIml , without obvious dynamic changes, but a depressed tendency was found after parturition. Ewes in late gestation showed high levels of IGF-1, its average was significantly higher than that for post-parturition period. During that period the plasma IGF-2 positively correlated to plasma glucose, and the correlation between IGF-1 and glucose was negative. Cholic acid infusion caused elevation in plasma IGF-2, but a few days later decreased, near the partuntion again rose, and then kept high levels during post-parturition period. The average of plasma IGF-1 in CA group (625.28 ?85.S6nglml) was obviously lower than that of control (705.21 ?52.24ng/ml) during pre-parturition period. CA-treated ewes also showed higher plasma glucose and a-amino nireogen levels, as well as sharp elevations of plasma IGF-2, insulin and cortisol at partuntion 3 period. It was concluded that maternal plasma IGF-1 is a greater regulator of fetal growth during late gestation and may have physiological blood glucose-Iowed effect as like as insulin, while plasma IGF-2 is relative unimportant determiner in contrast to IGF- 1, and its concentration may be regulated by plasma glucose. The rise in plasma IGF-2, insulin and cortisol after CA infusion relate to the stress, dystocia or abortion, while decreased maternal IGF-1 is one reason for intrauterine growth retardation. 2. RelatIonship between the plau. ItF? and inmtabolitea, hornunes in pregnant owes, their fetuses and neonatal I.E. during prenatal period 7 ewes of certain gestation time were assigned into CA-treated (n=5) and control group (n=2), then 1.1 mg/kg of cholic acid or equivalent 0.9% saline were infused into the jugular vein of ewes on 90 and 104 days of gestation, respectively. Up to 120 days of gestation, all ewes and their fetuses were operatively installed jugular vein catheter for ewes and frontal tlbien artery catheter for fetuses. Blood samples were taken daily after the operation for analyzing IGF-2 and metabolites. Final fetal

Related Dissertations

  1. Research of Glycosaminoglycan from Perna Virids Effect on Blood Lipid,R285.5
  2. Cloning and Expression of Growth Hormone Gene in Cobia (Rachycentron Canadus),Q786
  3. Protein phosphatase 2A Cα subunit knockout cardiac energy metabolism due to remodeling,Q78
  4. Effects of Different Reconciled Corn Oils on Antioxidative Stress, Lipid Metabolism Enzymes, and Abundance of Immune-related Genes of Broilers,S831.5
  5. Stereoselective Metabolism of Tetrahydropalmatine and Its Effect on Hepatic Drug Metabolism Enzymes,R96
  6. Effects of Enzymes on Metabolism of Polycyclic Aromatic Hydrocarbons in Plant,X173
  7. Cloning, Expression and Polymorphism Analysis of BMP4 Gene in Hu Sheep,S826
  8. GIH cDNA Cloning and Mrna Expression in Litopenaeus Vannamei,S917.4
  9. The Effect of Nitrogen Phosphate and Potassium Proportion Fertilizers on Tea Tree Physiological Metabolism and Tea Quality,S571.1
  10. The Difference of Proteome Expression of Low Light Stress and Regular Light Recovery on Growth of Maize Seedling,S513
  11. Coparative Analyses of Carbon and Nitrogen Metabolism of Flue-cured Tobacco Growing in Henan and Yunnan,S572
  12. Study on the Effect of Creatine Monohydrates on Carcass Composition, Meat Quality and Energy Metabolism of Postmortem Muscle in Duck,S834.5
  13. Effects of Varying Levels of Potassium Supplementation to the Low Salinity Waters or Diets on Growth and Physiological Characteristics of Shrimp, Litopenaeus Vannamei, Boone,S963
  14. Toxicities of Several Newly-Typed Insecticides to Colorado Potato Beetle, Leptinotarsa Decemlineata (Say) and Molecular Cloning of Two Kinds of Targets,S435.32
  15. Effects of Shading on Growth and Nitrogen Metabolism in Cotton Plant (Gossypium Hirsutum L.) during Flowering and Boll-Forming Stage,S562
  16. Effects of Nitrogen Management Practices on Expression and Degradation of Bt-protein in Transgenic Cotton,S562
  17. Research on Characteristics of Growth, Development and Material Metabolism of Flue Cured Tobacco Leaves in Huili, Sichuan,S572
  18. Effects of Soil C/N with Increasing Compost Wheat Straw on the Carbon and Nitrogen Metabolism and the Quality of Flue-cured Tobacco Leaves,S572
  19. The Study on Rules of Carbon and Nitrogen Metabolisms and Its Relationship with Flavor Quality of Strong Aroma Style Flue-cured Tobacco in Henan,S572
  20. Effects of Different Models of Cultivation Management on Nitrogen and Carbon Metabolism and Grain Yield in Winter Wheat,S512.1
  21. Study on the Regulatory Effects of Nitrogen Application Period on Yield Formation and Quality with Wheat,S512.1

CLC: > Biological Sciences > Botany > Plant Cell Genetics
© 2012 www.DissertationTopic.Net  Mobile