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Experimental Research on the Effect of Iodine Excess on the Morphology and Thyroid Function

Author: GaoTianShu
Tutor: ZuoWeiPing
School: China Medical University
Course: Internal Medicine
Keywords: Iodine excess Thyroid function Thyroid follicular epithelial morphology TSH TT4 TT3 Semi-quantitative research Ultrastructure MUI Apoptosis TUNEL Fas FasL Immunohistochemistry In situ hybridization FasmRNA FasLmRNA
CLC: R581
Type: PhD thesis
Year: 2002
Downloads: 306
Quote: 2
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Purpose Iodine is an essential trace element, the main raw material for the synthesis of thyroid hormones, is an important factor in the maintenance of normal thyroid function and morphology play an important regulatory role of thyroid hormone synthesis and release. Insufficient iodine intake can cause iodine deficiency disorders (iodine deficiency disorders, IDD) - thyroid hypofunction and brain developmental disorders. Since 1990, the use of iodized salt in the countries affected by IDD threat increase dramatically. The side effects of iodine excess are increasingly attracted the attention of scholars at home and abroad. Epidemiological investigation showed that iodine excess to increase iodine-induced hyperthyroidism (Iodine-induced hyperthyroidism, IIH), autoimmune thyroid disease (Autoimmune thyroid diseases, AITD), the prevalence of hypothyroidism, goiter and papillary thyroid cancer rate. Our areas of mild iodine deficiency (median urinary iodine MUI 103 micrograms / L), moderate iodine intake (MUI 374 micrograms / L) and high iodine intake area (MUI 614 micrograms / L) Children and adult thyroid function Comparative Study results show that iodine excess of children suffering from an increase in the prevalence of subclinical hypothyroidism, adult clinical hypothyroidism and subclinical hypothyroidism prevalence was significantly increased. Clinical hypothyroidism is mostly caused by autoimmune origin, subclinical hypothyroidism 1/3 related to autoimmunity, 2/3 may be related to iodine excess inhibition. Animal studies of iodine excess on thyroid function is two-fold excess of autoimmune disease susceptible animals thyroid function and iodine iodine excess on ordinary animal thyroid function. More on the latter on the former is less, and the results there is a contradiction. Animal experiments with the iodine dose is too high, the lack of MUI in the 300-600 micrograms / L study, the lack of urinary iodine monitoring, lack of iodine excess on thyroid morphology of semi-quantitative research, the lack of common animals and Epidemiology match experimental research; Several recent studies have shown that the formation of goiter and goiter caused by iodine deficiency and PTU recovery early Fas and FasL involved in the induction of apoptosis in thyroid cells, but did not see the long-term iodine excess on iodine deficiency and non-iodine deficiency ordinary the animal thyroid follicular cell Fas / FasL impact study; not seen by immunohistochemistry and in situ hybridization study from the overall level of iodine excess on Wistar rat thyroid follicular cell apoptosis and apoptosis genes Fas / FasL expression in thyroid follicular epithelial cells; iodized table salt with iodate there are different views. But the lack of Comparative Study on Effects of potassium iodate and potassium iodide excess on thyroid function and morphology. Iodine molecules are the field travelers and space pilots commonly used drinking water disinfectant, iodine molecules and iodide, in the case of excessive intake of the body thyroid function damage, has not been animal experiments report. Thus, we observed Mul 300 and 600 micrograms / L Wistar rat thyroid function and changes in body weight, and further semi-quantitative method of Wistar rat thyroid follicular epithelial cell morphology changes; comparing different iodine on thyroid function and Morphology of the difference, by immunohistochemistry and in situ hybridization study from the overall level of iodine excess on the the Wistal rat thyroid follicular epithelial cell apoptosis and apoptosis genes to F class squeeze FasL expression in thyroid follicular epithelial cells affected. Method 1 preparation and handling of animal models of factors: a low iodine animal model: the election Wis-shan, 4-week-old rats, male and female, were fed a normal diet of the animal department of my school, and feed containing 1% over of potassium chlorate double distilled water ( Double click stilled water group, later referred to DDW) 3 weeks, 3 weeks after disabled potassium chlorate, were randomly divided into 3 groups, respectively, to the DDW, iodine three times and six times the iodine concentration DDW formulated with potassium iodate, respectively, to iodine 7 days, 21 days, 90 days after the animals were killed. Non-iodine deficiency animal models selected 7-week-old Wistar rats were randomly grouped to DDW and 1 times, 2 times, 3 times, 6 times, 10 times, 20 times the concentration of iodine DDW, potassium iodate preparation, the animals were killed in iodine after 21 and 90 days, respectively. 3 times, 6 times the potassium iodide and molecular iodine concentration DDW group animals were sacrificed 90 days after the iodine. (2) In this study, the solid-phase immunoradiometric assay method serum, ISH, radioimmunoassay of serum bamboo 4, Tr3. Industry standards of the Ministry of Health of the People's Republic of China determined the median urinary iodine (MUI), observed each group Wistar rats iodine nutrition status. Each group were randomly selected 5 6 rat thyroid, HE staining. Under an optical microscope to observe the morphological changes of the thyroid follicular epithelial cells. Thyroid histological changes were scored using a semi-quantitative method. UIC Meta the morph image analysis system / o lympus of (DP10) / microscope (BX51) measurement of the area of ??each group of Wistar rat thyroid follicular epithelial cell height, follicular cavity. The end of the experiment, each elect a rat thyroid production of ultra-thin sections, transmission electron microscopy the ultrastructure of the thyroid follicular epithelial cells. Each group were randomly selected 5 6 Wistar rat thyroid by immunohistochemical SP method determination-Fas expression of FasL in the Wistar rat thyroid follicular epithelial cells. Each group five Wistar rats with TUNEL (the the deoxyribose nuclear past the end of the acid transfer mediated in situ nick end tag) Determination of thyroid follicular epithelial cell apoptosis. Wistar rats randomly selected from iodine deficiency and non-iodine deficiency 3 and 6 times higher iodine group, n = 5, in situ hybridization analysis make up 3 times, six times the iodine on thyroid follicular epithelial cells of Fas / FasL mRNA expression . 1 short-term compensated 3 times, 6 times iodine (7, 21 days) iodine deficiency Wistar rats serum few, Tr4 significantly reduced, but no significant effect on serum Qiao H. Long-term (90 days) to iodine deficiency Wistar rats 3 and 6 times higher iodine can be significantly reduced serum bamboo T a few significantly higher the clever H was significantly lower than the control group. Complement 3 and 6 times higher iodine 7,21 and 90 days failed to make the low-iodine goiter fully restored instead re-formation of goiter. 90 days make up more than three times the iodine iodine deficiency Wistar rats body weight had no significant effect. (2) long-term (90 days) more than 3 times to non iodine deficiency Wistal rats iodine (iodine intake concentration gt; 840 forest expansion L the MUI gt; 300 forest expansion L) in serum plant r few a few significantly reduced significantly higher, serum servant H also tended to increase, but no significant difference. Complement iodine more than 3 times 90 days?

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CLC: > Medicine, health > Internal Medicine > Endocrine diseases and metabolic diseases > Thyroid disease
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