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Study on Shijianchuan Treating Experimental Endometriosis

Author: FangFengQi
Tutor: ZhuFengQuan
School: Jilin University
Course: Obstetrics and Gynaecology
Keywords: Ectopic endometrial Endometrial Endometriosis Eutopic endometrial Ectopic lesions Local tissue Endocrine function E-Cadherin Experimental study c-myc
CLC: R711.71
Type: PhD thesis
Year: 2004
Downloads: 195
Quote: 3
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Abstract


Endometriosis (EM) Obstetrics and Gynecology common and difficult diseases, the incidence in recent years has significantly increased year by year trend. EM is not only one of the major cause of infertility among women, and a small number of endometrial cysts malignant transformation may occur, causing a serious impact on the quality of life of women of childbearing age. Iwami wearing Chinese medicine has the effect of promoting blood circulation, and this experiment for the first time for the treatment of EM. Using rat EM autologous transplantation animal model, experimental means pathology, immunopathology and molecular biology, from light and electron microscopy, endocrine, invasion mechanism, apoptosis and apoptosis-related gene regulatory perspective, explore EM pathogenesis and Chinese medicine Iwami wear therapeutic EM mechanism. The results and conclusions of the study are as follows: 1 endometriosis model successfully established the present study, Wister rats were surgically implanted autologous method model of EM animals, from the film surgical transplantation in vivo experiments on 50 rats, tumor formation rate was 66%. With histologically confirmed, the transplanted tissue visible glandular epithelial and mesenchymal cells, remains the original morphology reliable model. lt; WP = 106 gt; the 2 stone see the the wear inhibition of endometriosis from the the endometriotic lesion volume change and histopathological and ultrastructural changes of the stone see through EM animal models. The results show that the the Iwami wear endometriotic lesion volume can be reduced, or even shrinking, can inhibit the growth of ectopic endometrial. Ultrastructural changes and found a rat model of ectopic endometrial glandular irregular glandular structures exist. By electron microscopy, Iwami the wear group ultrastructure varying degrees of change, but there is also a cell pyknosis, apoptotic morphological changes of the nuclear membrane shrinkage, chromatin condensation edge set as well as apoptotic bodies. Suggesting, the Iwami wear promote apoptosis EM ectopic endometrial cells. 3 Iwami wear EM to EM model endocrine function is an estrogen-dependent diseases, endocrine function changes will directly affect the outcome of ectopic lesions. In this study, using radioimmunoassay (RIA) method for the determination stone see through to reduce the EM rats FSH, LH, E2 role, but is nowhere near Alarelin, stone see through vaginal smears of rats after treatment still visible cycle sex change ovulatory function in rats did not affected the endocrine function remained stable. So, consider stone see through the treatment of EM both low estrogen state to inhibit the growth of ectopic endometrium, but also to prevent a range of side effects of excessive estrogen low brought great therapeutic potential. 4 Iwami wear endometriosis adhesion, invasion, angiogenesis effects are now known in the intra-abdominal ectopic endometrial adhesion, planting, the growth mechanism is the the EM incidence of key. In this study, using immunohistochemical staining method to detect local tissue of rats in each group E-cadherin (E-Cadherin), matrix metalloproteinase -2 (MMP-2) and vascular endothelial growth factor (VEGF) expression. The experimental results show that the EM rat heterotopic endometrium, eutopic endometrium of E-Cadherin expression were significantly lower than in normal rats seen in EM, due to the decreased expression of E-cadherin, leading to cell depolymerization so utero The reduced adhesion between epithelial cells, and thus become easy dissociation from the normal position, \The stone see the wear and Alarelin can enhance the EM rats eutopic endometrium and ectopic endometrium expression of E-Cadherin. Iwami wear through enhanced local tissue adhesion, so easy to fall off, thereby reducing endometrial wander reduce lt; WP = 107 gt; EM invasion and metastasis. Endometrial adhesion decline must degrade the basement membrane and the extracellular matrix (ECM) ingredients to make the ectopic endometrium planting growth. MMP-2 which can degrade the ECM, the experimental results show that the EM rat eutopic endometrium, the ectopic endometrial expression of MMP-2 is higher than in normal rats, suggesting that high expression of MMP-2, so that the degradation of ECM, basement membrane The ability to easily penetrate the growing parts of the ECM in the destruction of the endometrium and ectopic endometrium obtained infiltration, planted. Iwami wear and Alarelin to reduce local tissue expression of MMP-2, thereby reducing the eutopic endometrium, ectopic endometrium degradation of ECM's role, so that it can not destroy the cells in the basement membrane, and further reduce its invasion, metastasis planting capacity. The countercurrent blood in the endometrial cells after adhesion and degradation EMC has been able to successfully Ectopic plant growth, angiogenesis involved in the mechanism of EM. The experimental results show that the EM rat heterotopic endometrium, the reign of endometrial VEGF expression were higher than normal rats, indicating that the improvement of EM rat the ectopic foci VEGF activity, so that the increase in intimal neovascularization might EM planting Growth determinants. The Iwami wear and Alarelin lower eutopic endometrial ectopic endometrial VEGF expression. Blocking its blood supply support, thereby inhibiting the formation of neovascular network of local organizations to further inhibit the growth of ectopic endometrium planting. The 5 the Iwami through apoptosis and its related genes of endometriosis because EM has characteristics similar to the invasion, metastasis and malignant tumors, so consider whether it also exists in EM inhibition of apoptosis performance. The experiment using TUNEL assay cell apoptosis of rats in each group of local organizations. The results show that the EM rat eutopic and ectopic endometrial sensitivity to apoptosis were significantly decreased ectopic endometrium compared with eutopic endometrium decreased more significantly, further confirmed ectopic endometrial cells of the anti-apoptotic stronger, so that it extend the survival time in the pelvic cavity, led to its cultivation in the pelvic and survival of endometriosis and development. Stone see through the treatment of EM significantly enhanced its the local organizations apoptosis sensitivity, visible Iwami ectopic lesions wear significantly reduced, endometrial atrophy thinning, may be achieved by inducing ectopic endometrial apoptosis 's. lt; WP = 108 gt; process of apoptosis is achieved by precise control of gene, which inhibit apoptosis gene Bcl-2 and promote the ratio between apoptotic gene Bax determines the sensitivity of cells to apoptosis. In this study, using in situ hybridization detection of local tissue of rats in each group Bcl-2 mRNA and Bax mRNA expression showed that EM rat ectopic endometrium Bcl-2 mRNA positive expression, and strong positive expression of Bax mRNA is only a small amount of expression, not even express. Visible ectopic endometrial cells the ability to enhance its own anti-apoptotic Bcl-2 gene expression and low expression of the Bax gene makes the ectopic endometrial able to survive outside the uterine cavity and planting, the formation of ectopic lesions . The Iwami wear, Alarelin treatment EM, ectopic lesions decreased expression of Bcl-2 mRNA and Bax mRNA expression enhanced visible, stone see through regulating Bcl-2/Bax expression to promote?

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CLC: > Medicine, health > Obstetrics and Gynaecology > Gynecology > Other diseases of the female genital > Endometriosis
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