Dissertation > Excellent graduate degree dissertation topics show

Study on Differentially Expressed Genes in the Salivary Gland of Hyalomma Asiaticum Tick

Author: ChengTianYin
Tutor: ShiQiShun;LinJiaoJiao
School: Hunan Agricultural University
Course: Animal Genetic Breeding and Reproduction
Keywords: Asian Hyalomma salivary glands Differentially expressed gene library GP29 gene GP15 gene Clone Expression
CLC: Q969.91
Type: PhD thesis
Year: 2005
Downloads: 105
Quote: 2
Read: Download Dissertation


Selected from a single female ticks breeding herd is not blood-sucking female adult ticks 60, were randomly divided into two groups, a group of half-blood meal, and the other group do not suck blood. Separation is not vampire and half full of blood state tick salivary glands. After total RNA extraction, first-strand synthesis, long-distance amplification, Ras I digestion, add joint, net of hybridization and selective amplification step, the isolated cDNA fragment of the differentially expressed genes. The pure product of the second amplification product into pGEM T-Easy vector and transformed into DH5a competent. 120 fragment identified by cloning and sequencing, and 84 of them identified as expressed sequence tags (EST). RT-PCR analysis confirmed that the library randomly selected 10 fragments in the blood-sucking female adult ticks salivary gland expression, not blood-sucking expression. Sequence alignment with the fragment of the NCBI database, the results were as follows: 120 fragments in the library, 90 to find a sequence having a certain similarity, wherein the similarity with other known genes ticks fragment 21, and press mosquito, Culex pipiens, hookworm, Ostertagia and other blood-sucking parasite gene fragments have a certain similarity 19. The ticks known genes Similar 21 fragment, the fragment is identified as EST 5 wherein the poly (A), 2 having ACGCGGGG; 2 is both Poly (A) with ACGCGGGG (that is, full-length gene, one of which has been receiving Genbank accession number DQ021902). The HAB1 and Ha7.7 similarity EST screened from the ticks known gene. According to HAB1 sequence design amplification primers HAB1 unknown end HAB1-GSP 1 and HAB1 GSP 2 . With HAB1-GSP 1 and UAP amplified by half full blood female adult tick salivary gland total RNA was reverse transcribed into the first strand, HaB1-GSP 2 and AUAP nested amplification, that was HAB1 unknown 3'-end. According to HAB1 its 3'-terminal sequence splicing results, design to GP 29 of the full-length amplification primers HAB1-FLP , HAB1-FLP - , in order to amplify the cDNA first strand the new gene GP 29 (accession number: AY803896) full-length. As design amplified Ha7.7 the 5'-end of the primer Ha7.7-GSP 1 , Ha7.7-GSP 2 , Ha7.7-GSP 3 and amplified GP 15 of the full-length primer Ha7.7 the for-FLP , Ha7.7-FLP - . The clone Ha7.7 obtaining the unknown part and full-length GP 15 (accession number: DQ020265). RT-PCR analysis showed that: Asian Hyalomma tick salivary glands only two genes expressed in the blood-sucking.

Related Dissertations

  1. Research on Combinatorial Regulation of Multiple Transcription Factors,Q78
  2. The Research of the High-Rise Building’s Form and Expression in the New Century,TU971
  3. Research on Design of Teaching Buildings for Arts Departments of Higher Learning Institutions,TU244.3
  4. Research on Temporal Information Recognition and Normalization,TP391.1
  5. Expression of D-AtCGS in E. Coli and Preparation of Polyclonal Antibody Against D-AtCGS,Q943.2
  6. The Study on Arabidopsis Thaliana Heat Shock Factor HSFA 1d Response to Formaldehyde Stress,Q945.78
  7. Expression of hBMP4 and hBMP7 in Chinese Hamster Ovary Cells,Q78
  8. Cloning and Expression Analysis of GPx, GST and SAHH Genes in Chlamydomonas Sp. ICE-L from Antarctica,Q943.2
  9. cDNA Cloning, Expression of vp5 and vp7 Genes and Subcecullar Localization of VP5 and VP7 Proteins in Grass Carp Reovirus,S941.41
  10. Cloning, Expression of vp6 and ns38 Genes and Immunogenicity of VP6 and NS38 in Grass Carp Reovirus,S941.41
  11. Expression of Prolyl Isomerase Pin1 in Osteosarcoma and the Effect of Regulation on Cell Cycle,R738.1
  12. The Expression Survey of BMP Signalling Pathway in the Human Embryonic Tooth Germ,R78
  13. Preliminary Study of Phosphorylation Effect on UGT1A3 Metabolism Activity Towards Drugs,R346
  14. Cloning and Expression of Growth Hormone Gene in Cobia (Rachycentron Canadus),Q786
  15. Cloning and Characterization of BAFF Gene from Whitespotted Catshark and Crucian Crap,S917.4
  16. Optimization of Fermentation Conditions, Purification, Cloning and Expression of a Cold-active Lipase from Pseudomonas Sp.RT-1,TQ925
  17. Functional Analysis of Proteins Encoded by RNA2 of Wheat Yellow Mosaic Virus,S435.121
  18. Comparative Study on Reproductive Biological Characteristics of Helicoverpa Armigera and Helicoverpa Assulta (Lepidoptera:Nuctuidae),S433
  19. Cloning, Expression and Polymorphism Analysis of BMP4 Gene in Hu Sheep,S826
  20. GIH cDNA Cloning and Mrna Expression in Litopenaeus Vannamei,S917.4
  21. Cloning and Expression Analysis of Scavenger Receptor Class B Type Ⅰ and Antifreeze Proteinstype Ⅱ Genes in Lutjanus Sanguineus,S917.4

CLC: > Biological Sciences > Entomology > Applied Entomology (Economic Entomology ) > Ticks and mites SCIENCE
© 2012 www.DissertationTopic.Net  Mobile