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Objective: To study the differentially expressed proteins in brain stem of seasickness adaptive rats and discuss its possible pathogenesis. Methods: Seasickness adaptive rat model was constructed according to the kaolin intakes after seasickness stimulation. The total brain stem proteins were isolated and identified by two-dimensional electrophoresis. Glutamine synthetase was confirmed by Western Blot. The glutamine synthetase activity, Glu, Asp, GABA and Gly contents were measured. Results: Sixteen seasickness adaptive proteins were identified by PMF: peroxiredoxin Ⅰ , peroxiredoxin Ⅱ, light molecular-weight neurofilament, ubiquitin carboxyl-terminal hydrolase PGP9.5, malate dehydrogenase, heme oxygenase 1, liver regeneration-related protein LRRG03, similar to sirtuin, hypothetical protein XP346711 and glutamine synthetase were highly expressed;carbonic anhydrase Ⅱ, triosephosphate isomerase Ⅰ , phosphoglycerate mutase isozyme B, M1 pyruvate kinase, Tpi1 and mitochondrial voltage dependent anion channel were lowly expressed. Moreover, the glutamine synthetase activity and glutamate content increased in adaptive rats. Conclusions: Adaptation to seasickness can induce changes of the protein expression pattern of brain stem in the seasickness adaptive rats, which may be associated with energy metabolism, neurotransmitter adjustmentand oxidative stress. Glutamate might play an important role in the seasickness adaptability formation.
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