Dissertation > Excellent graduate degree dissertation topics show

Effects and Mechanism of Vaporized Perfluorocarbon Therapy on Seawater Respiratory Distress Syndrome in Animal Models

Author: ZhaoXiaoWei
Tutor: LiuYouNing;ZhangJianPeng;JieLiXin
School: PLA Postgraduate Medical School
Course: Internal Medicine
Keywords: Sea water drowning -type respiratory distress syndrome Perfluorocarbon vaporization inhalation Surfactant protein End-expiratory pressure Surfactant Phospholipid Surface tension
CLC: R563.8
Type: PhD thesis
Year: 2008
Downloads: 50
Quote: 0
Read: Download Dissertation

Abstract


[OBJECTIVE]To investigate the effects of vaporized perfluorocarbon therapy on seawater respiratory distress syndrome in animal models and to approach the mechanism of the therapy.[METHODS]40 Famale Newsealand rabbits were randomly divided into five groups:(1)CMV group: received conventional ventilation, Vt=8ml/kg, PEEP=0cmH2O, PFC=0ml/kg.h; (2)PEEP group: received CMV and 8cmH2O positive end expiratory pressure (PEEP ) ventilation, Vt=8ml/kg , PEEP=8cmH2O, PFC=0mI/kg.h; (3)PFC group: received CMV and 7ml/kg.h PFC vaporized inhalation, Vt=8ml/kg, PEEP=0cmH2O, PFC=7ml/kg.h; (4)PFC+ PEEP group: received CMV, PEEP and PFC vaporized inhalation, Vt=8ml/kg, PEEP=8cmH2O , PFC=8ml/kg.h; (5)control group: without ventilation after making models, each group having 8 rabbits. The animals in former four groups received different treatment for 2 hours followed by conventional ventilation 4 hours. Then each animal was killed and underwent left lung lavage to analyse the change of interface tension , and right lung in same area were cut into small slides which partly were fixed in 10% buffered formalin and 2% glutaraldehyde to make morphometry evaluation, partly were storaged in liquid nitrogen to calculate molecular biology indexes. Other 3 rabbits were directly killed to make light microacopy studies.Part 1 To investigate the effects of vaporized perfluorocarbon on respiratory mechanics, hemodynamics mechanics and arterial blood gas indexes during and after different treatments. in addition, to observe the structural changes of lung after treatments.Part 2 In order to approach the mechanism of vaporized perfluorocarbon therapy, we evaluated surfactant biology function by interface tension of bronchial alveolar lavage fluids(BALF) measurement with captive bubble surfactometer, analysed the changes of surfactant composition , including the changes of phosphatides, that of SP-B in protein level and those of SP-B, SP-C mRNA expression in lung among different groups.[RESUILS]1 After lung injury, the data of respiratory mechanics and oxygenation index were significantly getting worsen. After receiving vaporized PFC, PEEP and PFC+PEEP ventilation, these indexes statistically improved compared with CMV in which there was no influence to the indexes. In addition, PFC and PEEP had synergistic effect on oxygenation improvement during treatment. After treatment, the improvement of oxygenation in PFC group could even persist long time, but the oxygenation indexes in PFC+PEEP group decreased to equal with those in PFC group in one hour. All animals in C group died in fifteen minites without ventilation.2 During treatment, PFC vapor had no influence in hemodynamics indexes. Compared C groups, lung injury and laminated body vacuolization decreased in PFC, PEEP, PFC+PEEP groups; in contrast , those got worsen in CMV group. 3 Among the five groups, the levels of interface tension in BALF in CMV and C groups were more increased than those in other three groups. After ten cycles, the minimum interface tension in PFC and PFC+PEEP groups decreased and were statistically lower than CMV and C two groups. There was no statistical difference in interface tension between PEEP group with CMV group, although the minimum interface tension in PEEP group decreased.4 PFC inhalation could influence the levels of differtent phosphatide composition , increasing phosphatidylcholine(PC) content and proportion, but not changing phosphatidylethanolamine (PE) level.5 In this study , we found that the mRNA expressions of SP-B and SP-C in PFC, PEEP and PFC+PEEP groups were significantly higher than those in other two groups, especially in PFC+PEEP group. which indicated vaporized PFC enhanced the mRNA expressions of SP-B and SP-C in lung. Western blot and IPHC results showed that vaporized PFC and PFC+PEEP treatment increased SP-B level in alveolar type II cell.6 Though analyzing the corrrlation coefficient of intersurface tension levels with the data of PE, PC, SP-B, SP-B mRNA, SP-CmRNA, we found that: the concentrations of SP-B mRNA, and SP-CmRNA had positive correlation with levels of intersurface tension.[CONCLUSION]1 Vaporized PFC , PEEP and vaporized PFC + PEEP ventilation could improve oxygenation indexes and lung compliane in SW-RDS animals, especially PFC + PEEP treatment during treatment. But after treatment, oxygenation indexes in group rapidly decreased and had no difference with those in PFC group in one hour.2 Vaporized PFC and PFC+PEEP ventilation enhanced the symehesis of lung surfactant , changing phosphatide composition, increasing SP-B, SP-C mRNA expression and SP-B level in lung, decrease inface tension and attenuate lung injury so as to regulate prognosis of seawater acute respiratory distress syndrome in animal models .

Related Dissertations

  1. Application of Additives’ Drag Reducing Technology in District Heating System,TU995
  2. Effectiveness Comparison of Different Solvents in Organochlorine Pesticide (OCP)-Contaminated Soil Flushing and Optimization of Flushing Parameters,X592
  3. Effects of Three Aquatic Plants on Soil Properties and Depletion of Rhizosphorus Nitrogen and Phosphorus,S154.4
  4. Adsortions of Dye by Modified Activated Carbon and Heavy Metals by Rice Husk-Based Activated Carbon,X703
  5. Synthesis and Properties of Functional Waterborne Polyurethane Coatings,TQ633
  6. Study on the Subsititute of Inorganic Salt Used in Active Dyeing on Cotton,TS193.5
  7. Sulfonic acid type gemini surfactants in the application of silicone emulsion,TQ316.3
  8. The Effects of Three Mechanical Ventilation Strategies on Inflammatory Factors and Type Ⅱ Alveolar Epithelium Cell Morphology in Newborn Piglets with Acute Lung Injury,R563.8
  9. The Mechanism Study on the Effect of Additives on the Structure of Reverse Osmosis Support Membrane,TB383.2
  10. Study on Breeding of Avilamycin Producing Strains and Conditions of Fermentation,TQ465
  11. Study on Insulin Phytosome and Self-microemulsion,R94
  12. Study on the Interaction of Lipid-Coated Quantum Dots with Cells,O471.1
  13. The Research of Coupled Arc AA-TIG Welding,TG444
  14. Studies on the Physical and Chemical Properties of Partially Hydrolyzed CPP-g-MAH,TQ320.1
  15. Research on the Permeability of Cuticle and Screening for Efficient Surfactant,Q942
  16. Research of the Fluorinated Temperature-sensitive Microgel Based on N-isopropylacrylamide,TQ316.334
  17. Synthesis and Characterization of Ordered Porous Silica Templated by Gemini Quaternary Ammonium Surfactants,O613.72
  18. Study on Synthesis and Properties of Carboxylate Gemini Surfactant,TQ423.11
  19. Kudzu its compound eye with the new formulation of,R286
  20. Study on the Surface Tension of Amine Aqueous Solution for CO2 Capture,X701
  21. Expression and Significance of S100A4、AnnexinA2 and Cathepsin B in the Breast Carcinoma,R737.9

CLC: > Medicine, health > Internal Medicine > Respiratory system and chest diseases > Pulmonary disease > Respiratory failure
© 2012 www.DissertationTopic.Net  Mobile