Dissertation > Excellent graduate degree dissertation topics show

New good attached side effect and mechanism of inhibition of transplanted liver cancer in mice

Author: ZuoLin
Tutor: ChenXinYi
School: Beijing University of Traditional Chinese Medicine
Course: Traditional Chinese Medicine
Keywords: Xinjia Liangfu recipe H22 hepatocarcinoma cell apoptosis gene vascular endothelial growth factor(VEGF) microvesseldensity(MVD)
CLC: R735.7
Type: PhD thesis
Year: 2008
Downloads: 165
Quote: 1
Read: Download Dissertation

Abstract


1. ObjectiveThe mice model of transplanted hepatocarcinorma H22 was chosen to observe the effect of tumor growth of transplanted hepatocarcinaoma on mice and expressions of Bcl-2,Bax proteins and vascular endothelial growth factor(VEGF), microvesseld- ensity(MVD) in tumor tissues.2. MethodKunming mice, transplanted by H22 hepatocarcinoma cells, were divided into a model group, a cyclophosphamide group and three groups of Xinjia Liangfu recipe in high dose (10g/kg), medium dose(5g/kg) and low dose(2.5g/kg). The dose of cyclophosphamide group was 17mg/kg, the model group received the same volume germfree saline. The mice were killed after the drugs and water had been given for twelve days and tumor tissues were separated. The size and weight of tumor tissues was measured and the tumor inhibition rate was calculated. And expressions of Bcl-2 , Bax and VEGF, MVD were detected using immuno- histochemical method.3. ResultThe study demonstrated the results as follows: I. The tumor inhibition rate of Xinjia Liangfu recipe group in high dose was 48.5% and was higher obviously than it of model group(P<0.01). II. Compared to the model group, the groups of Xinjia Liangfu recipe in high dose and medium dose could increase the expression of Bcl-2 protein and decrease the expression of Bax protein(P<0.01,P<0.05). III. The expression of VEGF influenced by Xinjia Liangfu recipe in high dose and medium dose were lower than that in model group(P<0.01). The Xinjia Liangfu recipe in medium dose could suppress the expression of MVD compared to it in model group (P<0.05).4. ConclusionXinjia Liangfu recipe could repress the growth of hepatocarcinorma. It could induce apoptosis of hepatocarcinorma cells through regulating down the expression of Bcl-2 and regulating up the expression of Bax. It also could inhibit the growth of tumor vein. Xinjia Liangfu recipe could induce apoptosis and inhibit growth of tumor vein to inhibit hepatocarcinorma.

Related Dissertations

  1. The Effects of Don on Proliferation, Differentiation, and Apoptosis of Chondrocytes in Chicken,S858.31
  2. Expressions and Relationships of PI3K/AKT and Apoptosis Related Regulation Factors in Epithelial Ovarian Tumors,R737.31
  3. The Analysis of the Growth Factor Related with the Secondary Glaucoma in the Anterior Champer of Silicone Oil-filled Eyes,R779.6
  4. Propranolol for Haemangiomas-insight into VEGF/ERK Signaling Transduction in HUVEC-12 Cells,R965
  5. Correlation between CT Perfusion Imaging in Nonsmall-cell Lung Cancer and VEGF、MVD、VEGF-C、LVD,R734.2
  6. The Expression of the Angiopoietin and Vascular Endothelial Growth Factor (VEGF) in Cervical Cancer and the Relationship with the Neogenetic Blood Vessel Density and Lymphatic Density,R737.33
  7. TP on the AIA rat VEGF, FLT1 and CCR5 expression,R684
  8. Effects of VEGF Toward to Formation of Vulnerable Atherosclerotic Plaque Interfered by Statins,R541.4
  9. The Effect of Ultrashort about the Rabbit with Steroid-Induced Avasular Necrosis of Femoral Head on VEGF mRNA and BMP-2 mRNA,R681.8
  10. The Observation of Macular Edema with OCT and Influence on Serum Level of VEGF in Retinal Vein Occlusion Patients with TCM Therapy Based on Stages,R276.7
  11. The Effect and Mechanism of Recombinant Human Endostatin in Proliferation and the Capacity of Invasion of Cervical Cancer Hela Cells,R96
  12. Study on the Suppressive Effect of miR-451 on Human Glioma Cell Growth in Vitro and Vivo,R739.4
  13. Expression of MiRNA-21 in Traumatic Brain Injury and Study of Its Intervention,R651.1
  14. Down-regulation of Telomerase Activity and Apoptosis of Nasopharyngeal Carcinoma Cells Induced by PinX1 Gene,R739.63
  15. Construction of Stathmin SiRNA Plasmid Expression Vector and the Effcts of Apoptosis and Cell Cycle in Npc 5-8F Cell by Silencing Expression of Stathmin Gene,R739.63
  16. Yiqihuoxue side of peptic ulcer mucosa HIF-1α, VEGF intervention effect,R285
  17. Research Biocompatibility of Osteoblasts with Nano Hydroxyapatite/Artificial Bone Collagen (NHAC) Adding Vascular Endothelial Growth Factor (VEGF),R318.08
  18. Experimental Studies of Injury to the Liver Tissue by Cyclopium Penicillic Toxin-PA,S816
  19. Effects of Gossypol Induced Luteal Cells Apoptosis in Mice,R114
  20. Expression and Prognostic Significance of Placental Growth Factor in Hepatocellular Carcinoma and Peritumoral Liver Tissue,R735.7
  21. Expression of SOCS3 in Thyroid Carcinoma and Its Clinical Significance,R736.1

CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Liver tumors
© 2012 www.DissertationTopic.Net  Mobile