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Folic acid is an important B vitamins , closely associated with many important biochemical processes directly affect the synthesis of nucleic acids and amino acid metabolism of cell division , proliferation and tissue growth has an extremely important role . The folate deficiency not only cause human megaloblastic anemia with neural tube defects , cancer, cardiovascular disease and other related . Therefore, the importance of folic acid in the diet and more people realize , infant food, food for the old , maternal food fortification . Microbiological method , colorimetry , chromatography method for determination of folic acid in foods . In this paper, by indirect fluorescence spectrometry and indirect atomic absorption spectrometry determination of the folic acid content . The establishment of a new method for the determination of drugs and food in the folic acid content . Determine the appropriate concentration range of the method and technology indicators . Film indirect fluorescence spectrometry determination of folic acid folic acid content : The results showed that the linear range of 0 ~ 1.6mg · L -1 , a detection limit of 51 μg · L -1 < / sup>, the average standard addition recovery was 97.1% (n = 6), and the relative standard deviation RSD = 1.99% ( n = 6 ) . This method is applied to pregnant women to strengthen the determination of folic acid in the milk powder to obtain a satisfactory result . Through extensive research show that folic acid in the appropriate conditions can with metallic ions Ag < / sup > and Mg 2 precipitate generated by the reaction , by measuring the absorbance of the remaining metal ions in the solution , indirect calculate the content of folic acid in the solution . This paper systematically studies indirect atoms of silver ions absorption law and magnesium ions indirect atomic absorption method for the determination of the content of folic acid . Two methods of standard addition recoveries were 96.8% to 102.8% and 97.5% to 101.2% , the relative standard deviation of 1.86% and 1.23 % , respectively ( n = 5 ) , two methods of detection limits were 3.3 μg · mL of -1 and 10.3μg · mL -1 , folic acid concentration of 0 ~ 0.1mg · L -1 , metal ions absorbance folate concentration is linear . Can be applied to actual samples . The equipment required for this study , simple and easy to get drugs , has a simple, rapid, selective advantages .
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